| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
|
|---|
| Outline of Final Research Achievements |
G-quadruplex (G4), a higher order structure of DNA, is involved in transcriptional regulation of genes, and detection of G4s in genome leads to the discoveries of new drug targets. Furthermore, selective stabilization of each G4 would become the selective control of the corresponding G4 function. Based on our macrocyclic polyoxazole type G4 ligand (6OTD, 7OTD), we have developed a new 7OTD analog attached with biotin-function. With the new ligand, we prepared a G4 affinity column, which enable us to detect the G4-forming DNA sequences in genome. In addition, we have developed new 6OTD derivatives for stabilizing two out of three representative topologies in G4 selectively.
|
