Imaging biomolecular dynamics verified using fluorogenic probes
| Project/Area Number |
26282215
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemical biology
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| Research Institution | Osaka University |
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Principal Investigator |
Hori Yuichiro 大阪大学, 工学研究科, 准教授 (00444563)
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| Research Collaborator |
NISHIURA Miyako
BABA Reisuke
HIRAYAMA Shinya
OTOMURA Norimichi
TAO Tomomi
GAO Jingchi
NISHIDA Ayuko
UMENO Maho
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2016: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2014: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Keywords | 発蛍光プローブ / GLUT4 / DNAメチル化 / PYPタグ / ハイブリッドプローブ / メチル化DNA / 細胞周期 / 機能性超分子プローブ / Regnase-1 |
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| Outline of Final Research Achievements |
Imaging of biomolecules is a useful method to elucidate biological phenomena in living cells. So far, fluorescent proteins have been used for live-cell imaging of proteins. In this research, to overcome limitations of existing methods, we developed fluorogenic probes, which emit fluorescence upon binding to biomolecules. By using the fluorogenic probes, we succeeded in analyzing protein trafficking, glycan function, and chemical modification of nucleic acids.
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Report
(5 results)
Research Products
(14 results)
