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Development of Optochemical Biology for photo-mediated regulation of gene function

Research Project

Project/Area Number 26282219
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Basic / Social brain science
Research InstitutionToho University

Principal Investigator

FURUTA Toshiaki  東邦大学, 理学部, 教授 (90231571)

Research Collaborator SUZUKI Akinobu  
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Keywordsケージド化合物 / 光化学 / 遺伝子 / 有機合成化学 / DNA / RNA / 遺伝子発現 / 有機化学 / ゲノム編集 / クリック反応 / PCR
Outline of Final Research Achievements

Photochemical activation of caged oligonucleotides enable the regulation of genes of interest within a single-cell level of spatial resolution. In the course of our study of the development of caged oligonucleotides, we designed and synthesized new precursor molecules of photo-releasable nucleotides having an affinity tag for purification and detection. A precursor molecule, Bio-Bhc-diazo comprises three components: biotin for an affinity tag, photoactive Bhc group, and phosphate reactive diazomethyl moiety. We developed a method to modify arbitrary sequence regions of long-chain double-stranded DNA and plasmid DNA with photolabile protecting groups by chemo-enzymatic reactions. We demonstrated that a caged compound of RNA can be synthesized using a novel nucleotide caging reagent having a biotin tag. We demonstrated that this technique can be applied to the gRNA of CRISPR / Cas9 system to synthesize caged gRNA protecting target DNA recognition site or Cas9 binding site.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (19 results)

All 2017 2016 2015 2014 Other

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (14 results) (of which Int'l Joint Research: 6 results,  Invited: 4 results) Remarks (1 results)

  • [Journal Article] A double bond-conjugated dimethylnitrobenzene-type photolabile nitric oxide donor with improved two-photon cross section2015

    • Author(s)
      1.N. Ieda, K. Hishikawa, K. Eto, K. Kitamura, M. Kawaguchi, T. Suzuki, K. Fukuhara, N. Miyata, T. Furuta, J. Nabekura, H. Nakagawa
    • Journal Title

      Bioorg. Med. Chem. Lett.

      Volume: 25 Issue: 16 Pages: 3172-3175

    • DOI

      10.1016/j.bmcl.2015.05.095

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Utilization of the Heavy Atom Effect for the Development of a Photosensitive 8-Azacoumarin-Type Photolabile Protecting Group2015

    • Author(s)
      Hikaru Takano, Tetsuo Narumi, Wataru Nomura, Toshiaki Furuta, and Hirokazu Tamamura
    • Journal Title

      Organic Letters

      Volume: 17 Issue: 21 Pages: 5372-5375

    • DOI

      10.1021/acs.orglett.5b02720

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Synthesis of nucleobase-caged peptide nucleic acids having improved photochemical properties2014

    • Author(s)
      T. Watanabe, T. Hoshida, J. Sakyo, M. Kishi, S. Tanabe, J. Matsuura, S. Akiyama, M. Nakata, Y. Tanabe, A. Suzuki, S. Watanabe, T. Furuta
    • Journal Title

      Org. Biomol. Chem.

      Volume: 12 Issue: 28 Pages: 5089-5093

    • DOI

      10.1039/c4ob00418c

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of the 8-aza-3-bromo-7-hydroxycoumarin-4-ylmethyl group as a new entry of photolabile protecting groups2014

    • Author(s)
      Takano, H., Narumi, T., Ohashi, N., Suzuki, A., Furuta, T., Nomura, W., Tamamura, H.
    • Journal Title

      Tetrahedron

      Volume: XX Issue: 29 Pages: 4400-4404

    • DOI

      10.1016/j.tet.2014.04.063

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] Design and synthesis of caged compounds with cell type specificity2017

    • Author(s)
      T. Furuta, A. Z. Suzuki, T. Sakano
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      仙台国際会議場(宮城県仙台市)
    • Year and Date
      2017-03-27
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Caged compounds as optochemical genetic tools -Design, synthesis and their use-2016

    • Author(s)
      T. Furuta
    • Organizer
      The 17th RIES-HOKUDAI International Symposium on Ju
    • Place of Presentation
      シャトレーゼガトーキングダムサッポロ(北海道札幌市)
    • Year and Date
      2016-12-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ynthesis of caged gRNAs for photoactivatable CRISPR-Cas92016

    • Author(s)
      K. Kodama, S. Tada, A. Z. Suzuki, T. Furuta
    • Organizer
      The Fourth Asian Chemical Biology Conference (ACBC2016)
    • Place of Presentation
      85 Sky Tower Hotel (Kaohsiung, TAIWAN)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Design and synthesis of site-selectively modified caged plasmid DNAs using modular nucleotide caging agents2016

    • Author(s)
      S. Tada, A. Z. Suzuki, T. Furuta
    • Organizer
      The Fourth Asian Chemical Biology Conference (ACBC2016)
    • Place of Presentation
      85 Sky Tower Hotel (Kaohsiung, TAIWAN)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] CRISPR/Cas9の光制御を目指したケージドgRNAの合成2016

    • Author(s)
      児玉一徳・多田慎之介・鈴木商信・古田寿昭
    • Organizer
      第6回CSJ化学フェスタ2016
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Year and Date
      2016-11-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Synthesis of caged gRNA for photoactivatable CRISPR/Cas92016

    • Author(s)
      K. Kodama, S. Tada, A. Suzuki, T. Furuta
    • Organizer
      日本化学会 第96春季年会 (2016)
    • Place of Presentation
      同志社大学京田辺キャンパス(京都府・京田辺市)
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] Design and synthesis of caged oligonucleotides with modular nucleotide caging agents2016

    • Author(s)
      S. Tada, A. Suzuki, T. Furuta
    • Organizer
      日本化学会 第96春季年会 (2016)
    • Place of Presentation
      同志社大学京田辺キャンパス(京都府・京田辺市)
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] Modular caging groups – new platforms for multifunctional caged compounds2015

    • Author(s)
      T. Furuta
    • Organizer
      The International Chemical Congress of Pacific Basin Societies 2015 (Pacifichem 2015)
    • Place of Presentation
      Hawaii (USA)
    • Year and Date
      2015-12-20
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Chemo-enzymatic preparation of site-selectively modified caged DNAs2015

    • Author(s)
      S. Tada, W. Hashiba, E. Ikeda, A. Suzuki, T. Furuta
    • Organizer
      The International Chemical Congress of Pacific Basin Societies 2015 (Pacifichem 2015)
    • Place of Presentation
      Hawaii (USA)
    • Year and Date
      2015-12-19
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Design and synthesis of clickable caged compounds2015

    • Author(s)
      A. Suzuki, R. Aikawa, K. Kakehi, T. Furuta
    • Organizer
      The International Chemical Congress of Pacific Basin Societies 2015 (Pacifichem 2015)
    • Place of Presentation
      Hawaii (USA)
    • Year and Date
      2015-12-19
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] プラスミドDNAに領域選択的に光分解性保護基を導入する手法の開発2015

    • Author(s)
      多田慎之介,古田寿昭
    • Organizer
      第5回CSJ化学フェスタ(2015)
    • Place of Presentation
      タワーホール船堀(東京都・江戸川区)
    • Year and Date
      2015-10-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] Design and synthesis of new caged diacylglycerol2015

    • Author(s)
      池谷柚季,森有里紗,鈴木商信,古田 寿昭
    • Organizer
      日本化学会第95春季年会(2015)
    • Place of Presentation
      日本大学理工学部船橋キャンパス/薬学部(千葉県船橋市)
    • Year and Date
      2015-03-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Chemical tools to control cellular chemistry2015

    • Author(s)
      Toshiaki Furuta
    • Organizer
      第92回 日本生理学会大会
    • Place of Presentation
      神戸国際会議場・展示場(兵庫県神戸市)
    • Year and Date
      2015-03-22
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ケージドDNAを化学合成するためのホスホロアミダイト試薬の合成2014

    • Author(s)
      池田英一郎,鈴木商信,古田寿昭
    • Organizer
      第4回CSJ化学フェスタ
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Year and Date
      2014-10-16
    • Related Report
      2014 Annual Research Report
  • [Remarks] 光で細胞機能を操作するOptochemical Biologyの創生

    • URL

      http://www.lab.toho-u.ac.jp/sci/biomol/tfuruta-lab/

    • Related Report
      2016 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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