| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Outline of Final Research Achievements |
Graphene is an excellent acceptor in fluorescence resonance energy transfer (FRET) process in visible light region. Using this characteristics, we developed a biosensor (graphene FRET aptasensor) that emit fluorescence when the target protein exists in a sample. We showed that the graphene FRET aptasensor yields more than 3 times greater fluorescence than GO sensor. We designed the sensor array with internal reference for quantitative detections by combining microfluidics technology to the graphene FRET aptasensor. We showed the limit of detection of the graphene FRET aptasensor is several hundred ng/mL. For the electron transfer reaction of graphene, we built a new surface modification for a substrate to strengthen adhesion between graphene and substrate though the electrochemical properties of graphene is remained.
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