Molecular structure mechanism of caveolae membrane coats

• Project/Area Number: 26286027
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Nanobioscience
• Research Institution: Kyoto University
• Principal Investigator: MORONE NOBUHIRO 京都大学, 物質－細胞統合システム拠点, 教授 (50399680)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000); Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
• Keywords: 1分子科学 / 細胞膜 / カベオラ / エンドサイトーシス / １分子科学

Outline of Final Research Achievements

Cavoelae are striated membrane invagination (around 70nm in diameter) at the plasma membrane except for neurons, which involved in endocytosis, transcytosis, and fatty acid uptake. Freeze-replica electron microscopy would be useful for caveolar analysis due to their fine structures on the cytoplasmic surface of plasma membrane. This research focused caveolar base structures by electron microscopy.

Research Products

• [Int'l Joint Research] The University of Queensland(Australia)
• [Int'l Joint Research] Dartmouth Medical School(米国)
• [Int'l Joint Research] Institut Curie(France)
• [Int'l Joint Research] Max Plank Institute(Germany)
• [Journal Article] Hybrid Cellular Metabolism Coordinated by Zic3 and Esrrb Synergistically Enhances Induction of Naive Pluripotency (2017)
  • Author(s): Sone M, Morone N, Nakamura T, Tanaka A, Okita K, Woltjen K, Nakagawa M, Heuser J, Yamada Y, Yamanaka S, Yamamoto T.
  • Journal Title: Cell Metabolism Volume: 印刷中 Issue: 5 Pages: 1103-1117.e6
  • DOI: 10.1016/j.cmet.2017.04.017
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The modeling of Alzheimer's disease by the overexpression of mutant Presenilin 1 in human embryonic stem cells. (2016)
  • Author(s): Honda M, Minami I, Tooi N, Morone N, Nishioka H, Uemura K, Kinoshita A, Heuser JE, Nakatsuji N, Aiba K.
  • Journal Title: Biochem Biophys Res Commun. Volume: 469 Issue: 3 Pages: 587-92
  • DOI: 10.1016/j.bbrc.2015.12.025
  • NAID: 120005980892
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Confined diffusion of transmembrane proteins and lipids induced by the same actin meshwork lining the plasma membrane (2016)
  • Author(s): T. K. Fujiwara, Iwasawa, K., Kalay, Z., Tsunoyama, T. A. Y. Watanabe, Y. M. Umemura, H. Murakoshi, K. G. N. Suzuki, Y. L., Nemoto, N. Morone, A. Kusumi
  • Journal Title: Mol. Cell Biol. Volume: 27 Issue: 7 Pages: 1101-1119
  • DOI: 10.1091/mbc.e15-04-0186
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Polymer-Coated pH-Responsive High-Density Lipoproteins (2016)
  • Author(s): Hyungjin Kim, Haruki Okamoto,Arnaud E Felber, Anna Polomsak, Nobuhiro Morone, John E Heuser, Jean-Christophe Leroux, Tatsuya Murakami
  • Journal Title: J. Control. Release Volume: 228 Pages: 132-140
  • DOI: 10.1016/j.jconrel.2016.03.005
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Metabolic reprogramming during neuronal differentiation. (2016)
  • Author(s): Agostini M, Romeo F, Inoue S, Niklison-Chirou MV, Elia AJ, Dinsdale D, Morone N, Knight RA, Mak TW, Melino G.
  • Journal Title: Cell Death Differ. Volume: 23 Issue: 9 Pages: 1502-14
  • DOI: 10.1038/cdd.2016.36
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Confined diffusion of transmembrane proteins and lipids induced by the same actin meshwork lining the plasma membrane. (2016)
  • Author(s): Fujiwara TK, Iwasawa K, Kalay Z, Tsunoyama TA, Watanabe Y, Umemura YM, Murakoshi H, Suzuki KG, Nemoto YL, Morone N, Kusumi A.
  • Journal Title: Mol Biol Cell Volume: 27 Pages: 1101-19
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Thermosensitive Ion Channel Activation in Single Neuronal Cells by Using Surface-Engineered Plasmonic Nanoparticles. (2015)
  • Author(s): Nakatsuji H, Numata T, Morone N, Kaneko S, Mori Y, Imahori H, Murakami T.
  • Journal Title: Angewandte Chemie International Edition Volume: 54 Issue: 40 Pages: 11725-1729
  • DOI: 10.1002/anie.201505534
  • NAID: 120005646471
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Diffusion-coupled molecular assembly: structuring of coordination polymers across multiple length scales. (2014)
  • Author(s): Hirai K, Reboul J, Morone N, Heuser JE, Furukawa S, Kitagawa S.
  • Journal Title: J Am Chem Soc. Volume: 136 Issue: 42 Pages: 14966-14973
  • DOI: 10.1021/ja507971r
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Regulation of cargo-selective endocytosis by dynamin 2 GTPase-activating protein girdin. (2014)
  • Author(s): Liang Weng, Atsushi Enomoto, Hiroshi Miyoshi, Kiyofumi Takahashi, Naoya Asai, Nobuhiro Morone, Ping Jiang, Jian An, Takuya Kato, Keisuke Kuroda, Takashi Watanabe, Masato Asai, Maki Ishida-Takagishi, Yoshiki Murakumo, Hideki Nakashima, Kozo Kaibuchi and Masahide Takahashi.
  • Journal Title: EMBO Journal. Volume: 33 Issue: 18 Pages: 2098-2112
  • DOI: 10.15252/embj.201488289
  • Peer Reviewed / Open Access
• [Journal Article] Mesoscopic Metal Nanoparticles Doubly Functionalized with natural and engineered lipidic dispersants for therapeutics (2014)
  • Author(s): Murakami, T., Nakatsuji, H., Morone, N., Heuser, J. E., Ishidate, F., Hashida, M., Imahori, H.
  • Journal Title: ACS Nano Volume: 8 Issue: 7 Pages: 7370-7376
  • DOI: 10.1021/nn5024818
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Helical DNA origami tubular structures with various sizes and arrangements (2014)
  • Author(s): M. Endo, S. Yamamoto, T. Emura, K. Hidaka, N. Morone, J. E. Heuser, H. Sugiyama
  • Journal Title: Angewandte Chemie, International Edition Volume: 53 Issue: 29 Pages: 7484-7490
  • DOI: 10.1002/anie.201402973
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 細胞やタンパク質分子複合体の無氷晶凍結に必要な基礎原理と方法論 (2016)
  • Author(s): 諸根信弘
  • Organizer: 日本顕微鏡学会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2016-06-14
  • Invited
• [Presentation] プラズモンナノ材料による1神経細胞の熱感受性イオンチャネルの光活性化 (2015)
  • Author(s): 村上 達也, 中辻 博貴, 沼田 朋大, 諸根 信弘, 金子 周司, 森 泰生, 今堀 博
  • Organizer: Biochemistry and Molecular Biology 2015
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2015-12-01
• [Presentation] Three dimensional structure of cytoskeleton in axon. (2015)
  • Author(s): Kuwahara R, Yokoyama K, Aoyama K, Mitsuoka K and Morone N.
  • Organizer: The 2nd East-Asia Microscopy Conference
  • Place of Presentation: 姫路、日本
  • Year and Date: 2015-11-24
  • Int'l Joint Research
• [Presentation] 神経軸索内膜骨格の3次元構造 (2015)
  • Author(s): 桒原 隆亮, 青山 一弘, 光岡 薫, 諸根 信弘
  • Organizer: 日本顕微鏡学会第７１回学術講演会
  • Place of Presentation: 京都、日本
  • Year and Date: 2015-05-13
• [Presentation] Cryo-STEM Tomography による細胞構造の3次元解析 (2015)
  • Author(s): 青山 一弘, 諸根 信弘, 桒原 隆亮, 横山 佳奈, 光岡 薫
  • Organizer: 日本顕微鏡学会第７１回学術講演会
  • Place of Presentation: 京都、日本
  • Year and Date: 2015-05-13
• [Presentation] カベオラと細胞膜の相互作用点に関する構造解析的検証 (2015)
  • Author(s): 諸根 信弘
  • Organizer: 日本顕微鏡学会第７１回学術講演会
  • Place of Presentation: 京都、日本
  • Year and Date: 2015-05-13
• [Presentation] 感染性タンパク質結晶化の細胞内メゾ構造解析 (2015)
  • Author(s): 諸根 信弘, 森 肇, Heuser John
  • Organizer: 日本顕微鏡学会第７１回学術講演会
  • Place of Presentation: 京都、日本
  • Year and Date: 2015-05-13
• [Presentation] Three dimensional structure of ring-shaped membrane skeleton in axon (2015)
  • Author(s): Kuwahara R, Aoyama K, Mitsuoka K and Morone N.
  • Organizer: 日本顕微鏡学会第７１回学術講演会
  • Place of Presentation: 京都、日本
  • Year and Date: 2015-05-13
• [Presentation] Intracellular crystallization of insect virus and polyhedra revealed by quick-freeze, freeze-fracture electron microscopy. (2014)
  • Author(s): Morone N, Mori Hajime, Heuser J.
  • Organizer: The 2014 ASCB/ ifcb meeting
  • Place of Presentation: Fohiladelphia, USA
  • Year and Date: 2014-12-06 – 2014-12-10
• [Presentation] Mesoscale organization of insect virus-polyhedra crystals revealed by rapid-freeze, freeze-fracture EM (2014)
  • Author(s): Morone N, Mori Hajime, Heuser J.
  • Organizer: 18th International Microscopy Congress
  • Place of Presentation: Prague, Czech
  • Year and Date: 2014-09-07 – 2014-09-12
• [Presentation] Insect virus-polydhedra crystals revealed by tepid-freeze, freeze-fracture EM. (2014)
  • Author(s): Morone N, Mori Hajime, Heuser J.
  • Organizer: 日本顕微鏡学会第７０回記念学術講演会
  • Place of Presentation: 幕張、日本
  • Year and Date: 2014-05-11 – 2014-05-13