Cell Origami-3D cell-laden structures using origami folding technique
| Project/Area Number |
26286030
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Nano/Microsystems
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
岩瀬 英治 早稲田大学, 理工学術院, 准教授 (70436559)
尾上 弘晃 慶應義塾大学, 理工学部, 講師 (30548681)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2016: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2014: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
|
|---|
| Keywords | 折紙工学 / MEMS / 再生医療 / マイクロ・ナノデバイス / 折り紙工学 |
|---|
| Outline of Final Research Achievements |
1)We successfully produced origami-inspired method to generate 3D cell laden microstructures using MEMS technique. 2) Magnetic material is embedded in the microplate, and a magnetic field is applied from the outside to control the shape and timing of folding, which makes it possible to change the shape of active cells in the cell culture process. 3)The stem cells dedifferentiated by adipocytes were cultured on the microplate and are folded. We found that the cells differentiated more likely into bones than when cultured on a flat surface. 4) We successfully produced 3D microstructures of co-culture fibroblasts (NIH/3T3 cells) and hepatocytes (HepG2 cells). Measurement of secreted albumin confirmed HepG2 function in the 3D microstructures at 5 and 7 days after formation. 5) We found that repeating folding and deployment processes for NIH/3T3 that were cultured on the active micro device affected the orientation of actin in the cells.
|
Report
(4 results)
Research Products
(31 results)
