Real-time in situ measurements of free radicals during Plasma sterilization

• Project/Area Number: 26286071
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Plasma electronics
• Research Institution: Nagoya University
• Principal Investigator: Ishikawa Kenji 名古屋大学, 工学(系)研究科(研究院), 教授 (60417384)
• Co-Investigator(Renkei-kenkyūsha): OHTA Takayuki 名城大学, 理工学部, 准教授 (10379612) ; TANAKA Hiromasa 名古屋大学, 大学院工学研究科, 特任講師 (00508129)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000); Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2014: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: プラズマ滅菌 / 電子スピン共鳴 / プラズマ化学 / 代謝解析 / プラズマ加工 / 代謝

Outline of Final Research Achievements

The low-temperature plasma sterilization have advantages for applications. However, complexity of plasma processes arises from the involvement of a myriad of species including: electrons, ions, radicals, and photons. Such plasma components are needed to be measured and analyzed for fine-tuning the process. To determine the best practice for the sterilization, plasma diagnostics were conducted on clarifying the relationship between the control parameters and the plasma parameters.
Penicillium digitatum spores, which causes green mold on citrus, are inactivated by contribution of extracellular reactive oxygen species (ROS). The stable free radical as semiquinone in the spores decreases during oxygen atom irradiation observed using real time in situ electron spin resonance (ESR). The plasma electrical discharge induce metabolic modification. Also, intracellular metabolites induce the inactivation of spores. These understanding provides the development of plasma sources.

Research Products

• [Journal Article] Cold plasma interactions with enzymes in foods and model systems (2016)
  • Author(s): N. N. Misra, S. K. Pankaj, Annalisa Segat, Kenji Ishikawa
  • Journal Title: Trends in Food Science & Technology Volume: 55 Pages: 39-47
  • DOI: 10.1016/j.tifs.2016.07.001
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Quantitative clarification of inactivation mechanism of Penicillium digitatum spores treated with neutral oxygen radicals (2014)
  • Author(s): Hiroshi Hashizume, Takayuki Ohta, Keigo Takeda, Kenji Ishikawa, Masaru Hori, Masafumi Ito
  • Journal Title: Jpn. J. Appl. Phys. Volume: 54 Issue: 1S Pages: 01AG05-01AG05
  • DOI: 10.7567/jjap.54.01ag05
  • Peer Reviewed / Open Access
• [Presentation] 医療・バイオ応用プラズマ技術 (2017)
  • Author(s): 石川健治
  • Organizer: 電子情報技術部会 エレクトロニクス交流会エレクトロニクス応用技術講演会「プラズマエレクトロニクスの新展開」
  • Place of Presentation: 東京都千代田区
  • Year and Date: 2017-03-29
  • Invited
• [Presentation] Plasma technology for medicine and bio application (2017)
  • Author(s): Kenji Ishikawa
  • Organizer: International Workshop on advanced plasma applications on bio and nanomaterials
  • Place of Presentation: Nagoya, Japan
  • Int'l Joint Research / Invited
• [Presentation] Plasma-activated Medium (PAM) and Metabolic Analysis of Glioblastoma (U251SP) (2016)
  • Author(s): Kenji Ishikawa
  • Organizer: International Symposium on Material Research Society Japan (MRS-J)
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2016-12-19
  • Int'l Joint Research / Invited
• [Presentation] なぜプラズマは生体に作用するか (2016)
  • Author(s): 石川健治
  • Organizer: 第１７回プラズマ医療 サイエンスの扉＆サイエンスカフェ
  • Place of Presentation: 名古屋
  • Year and Date: 2016-12-16
  • Invited
• [Presentation] CE-TOF-MS analysis of aerobic glycolysis metabolism on glioblastoma (U251SP) modified in electrically discharged plasma activated medium (PAM) cultivation (2016)
  • Author(s): Kenji Ishikawa
  • Organizer: 6th NU-SKKU Joint Symposium on Neuroscience was held at Center of Neuroscience Imaging Research
  • Place of Presentation: Suwon, Korea
  • Year and Date: 2016-11-24
  • Int'l Joint Research / Invited
• [Presentation] プラズマが照射された液体中の活性種の分析 (2016)
  • Author(s): 石川健治
  • Organizer: プラズマ・核融合学会第33回年会
  • Place of Presentation: 仙台
  • Year and Date: 2016-11-24
  • Invited
• [Presentation] Metabolic profiles on glioblastoma (U251SP) modified in plasma-activated medium (PAM) cultivation (2016)
  • Author(s): Kenji Ishikawa, N. Kurake, H. Tanaka, H. Hashizume, K. Takeda, K. Nakamura, H. Kajiyama, H. Kondo, M. Sekine, M. Kato, M. Mizuno, F. Kikkawa, M. Hori
  • Organizer: International Conference on Plasma Medicine (ICPM)
  • Place of Presentation: Blatislava, Slovakia
  • Year and Date: 2016-09-04
• [Presentation] プラズマ活性化溶液(PAM)培養した 脳腫瘍細胞(U251SP)の 代謝プロファイル (2016)
  • Author(s): 石川健治, 倉家 尚之, 田中 宏昌, 橋爪 博司, 竹田 圭吾, 中村 香江, 梶山 広明, 近藤 博基, 関根 誠, 加藤 昌志, 水野 正明, 吉川 史隆, 堀 勝
  • Organizer: 日本酸化ストレス学会学術集会
  • Place of Presentation: 仙台
  • Year and Date: 2016-08-30
• [Presentation] Latent Chemistry in Plasma activated medium (PAM) (2016)
  • Author(s): Kenji Ishikawa
  • Organizer: 20th International Vacuum congress (IVC-20)
  • Place of Presentation: Busan, Korea
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] Diagnostics of activated species generated by non-equilibrium atmospheric pressure plasmas for plasma biomedical applications (2016)
  • Author(s): Kenji Ishikawa
  • Organizer: International Workshop on Plasma Spectroscopy (IPS)
  • Place of Presentation: Inuyama, Japan
  • Year and Date: 2016-06-26
  • Int'l Joint Research / Invited
• [Presentation] Sterilization efficiency enhancement by using magnet-assisted-plasma jet (2016)
  • Author(s): Chih-Tung Liu, Takumi Kumakura, Kenji Ishikawa, Hiroshi Hashizume, Keigo Takeda, Masafumi Ito, Masaru Hori, and Jong-Shinn Wu
  • Organizer: ISPlasma 2016/9th IC-PLANTS
  • Place of Presentation: Nagoya University
  • Year and Date: 2016-03-04
  • Int'l Joint Research
• [Presentation] 医療バイオ応用プラズマ (2015)
  • Author(s): 石川健治
  • Organizer: 第２６回プラズマエレクトロニクス講習会
  • Place of Presentation: 東京大学武田先端知
  • Year and Date: 2015-12-20
  • Invited
• [Presentation] Nonlinear optical spectroscopic measurements of plasma-treated bio-surfaces (2015)
  • Author(s): Kenji Ishikawa, Ryo Furuta, K. Takeda, T. Ohta, H. Hashizume, H. Kondo, M. Ito, M. Sekine, and M. Hori
  • Organizer: 第25回日本MRS年次大会 国際シンポジウムC-5
  • Place of Presentation: 横浜市開港記念会館、横浜情報文化センター
  • Year and Date: 2015-12-08
  • Int'l Joint Research
• [Presentation] Nonlinear Optical Spectroscopic Observation of Plasma-Treated Bio- Specimen (2015)
  • Author(s): Kenji Ishikawa, Ryo Furuta, K. Takeda, T. Ohta, H. Hashizume, H. Kondo, M. Ito, M. Sekine, and M. Hori
  • Organizer: International Symposimum on American Vacuum Scoiety (AVS)
  • Place of Presentation: San Jose, CA, USA
  • Year and Date: 2015-10-18
  • Int'l Joint Research
• [Presentation] Sterilization efficiency of the Atmospheric Pressure Jet Entrained Ambient Air (2015)
  • Author(s): Chih-Tung Liu, Meng-Hua Hu, Kenji Ishikawa, Masaru Hori, J. S. Wu
  • Organizer: 第７６回応用物理学会秋季学術講演会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2015-09-13
• [Presentation] 非平衡大気圧プラズマ照射培養液中の酸窒化活性種の計測と抗腫瘍効果 (2015)
  • Author(s): 石川 健治 倉家 尚之, 田中 宏昌, 近藤 隆, 水野 寛子, 大沼 章子, 加藤 昌志, 中村 香江, 梶山 広明, 竹田 圭吾, 近藤 博基, 関根 誠, 吉川 史隆, 水野 正明, 堀 勝
  • Organizer: 第６８回日本酸化ストレス学会学術集会
  • Place of Presentation: 鹿児島県民交流センター
  • Year and Date: 2015-06-11
• [Presentation] Plasma-Biological Surface Interaction for Food Hygiene (2014)
  • Author(s): Kenji Ishikawa, Hiroshi Hashizume, Takayuki Ohta, Masafumi Ito, Keigo Takeda, Hiromasa Tanaka, Hiroki Kondo, Makoto Sekine, and Masaru Hori
  • Organizer: E-MRS and MRS-J Joint Symposium
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2014-12-10 – 2014-12-12
  • Invited
• [Presentation] Electron spin resonance（ESR） Study of edible meat irradiated atmospherics-pressure-plasmas (2014)
  • Author(s): Kenji Ishikawa, Hiroshi Hashizume, Takayuki Ohta, Masafumi Ito, Hiromasa Tanaka, Keigo Takeda, Hiroki Kondo, Makoto Sekine, and Masaru Hori
  • Organizer: IUMRS-ICA Symposium D-2 Frontier of Nano-Materials Based on Advanced Plasma Technologies
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 2014-08-26 – 2014-08-31
• [Presentation] Plasma-Biological Material Interactions Studied by Employing Electron Spin Resonance (ESR) Technique (2014)
  • Author(s): Kenji Ishikawa, Hiroshi Hashizume, Takayuki Ohta, Masafumi Ito, Hiromasa Tanaka, Keigo Takeda, Satomi Tajima, Hiroki Kondo, Makoto Sekine, and Masaru Hori
  • Organizer: The XXII Europhysics Conference on Atomic and Molecular Physics of Ionized Gases (ESCAMPIG)
  • Place of Presentation: Greifswald, Germany
  • Year and Date: 2014-07-15 – 2014-07-19
• [Presentation] Microscopic observation of inactivation process of fungal spores treated with oxygen radicals produced from an atmospheric-pressure plasma (2014)
  • Author(s): Hiroshi Hashizume, Takuya Towatari, Takayuki Ohta, Keigo Takeda, Kenji Ishikawa, Masaru Hori, and Masafumi Ito
  • Organizer: The 17th Biennial Meeting for the Society for Free Radical Research International (SFRRI 2014)
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2014-05-24 – 2014-05-25
• [Presentation] Quantitative evaluation of the inactivation process of P. digitatumspores on the basis of dose of ground-state atomic oxygen (2014)
  • Author(s): Hiroshi Hashizume, Takuya Towatari, Takayuki Ohta, Keigo Takeda, Kenji Ishikawa, Masaru Hori, and Masafumi Ito
  • Organizer: International Workshop on Diagnostics and Modelling for Plasma Medicine (DMPM2014)
  • Place of Presentation: Nara, Japan
  • Year and Date: 2014-05-23 – 2014-05-24
  • Invited
• [Presentation] Inactivation process of P. digitatum spores evaluated by dose of ground-state atomic oxygen (2014)
  • Author(s): Hiroshi Hashizume, Takuya Towatari, Takayuki Ohta, Keigo Takeda, Kenji Ishikawa, Masaru Hori, and Masafumi Ito
  • Organizer: The 5th International Conference on Plasma Medicine (ICPM5)
  • Place of Presentation: Nara, Japan
  • Year and Date: 2014-05-17 – 2014-05-23
• [Presentation] Inactivation of microorganism in liquid treated with neutral reactive oxygen species (2014)
  • Author(s): Takuya Towatari, Hiroshi Hashizume, Takayuki Ohta, Keigo Takeda, Kenji Ishikawa, Masaru Hori, and Masafumi Ito
  • Organizer: The 5th International Conference on Plasma Medicine (ICPM5)
  • Place of Presentation: Nara, Japan
  • Year and Date: 2014-05-17 – 2014-05-23
• [Presentation] Electron Spin Resonance Study of Plasma-Biological Surface Interactions under Atmospheric Pressure Plasmas (2014)
  • Author(s): Kenji Ishikawa, Hiroshi Hashizume, Takayuki Ohta, Masafumi Ito, Hiromasa Tanaka, Keigo Takeda, Satomi Tajima, Hiroki Kondo, Makoto Sekine, and Masaru Hori
  • Organizer: The 5th International Conference on Plasma Medicine (ICPM5)
  • Place of Presentation: Nara, Japan
  • Year and Date: 2014-05-17 – 2014-05-23