Anisotropic function of antifreeze protein for ice crystal growth - To elucidate growth promotion and inhibition mechanism

• Project/Area Number: 26287095
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biological physics/Chemical physics/Soft matter physics
• Research Institution: Hokkaido University
• Principal Investigator: FURUKAWA Yoshinori 北海道大学, 低温科学研究所, 名誉教授 (20113623)
• Co-Investigator(Kenkyū-buntansha): 佐崎 元 北海道大学, 低温科学研究所, 教授 (60261509) ; 長嶋 剣 北海道大学, 低温科学研究所, 助教 (60436079) ; 村田 憲一郎 北海道大学, 低温科学研究所, 助教 (60646272)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000); Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2014: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
• Keywords: 氷結晶 / 不凍タンパク質 / 界面吸着 / 結晶成長 / 異方的不純物効果 / 振動成長 / 成長速度促進 / 凍結抑制タンパク質 / 不純物効果 / 成長促進 / 成長速度の促進 / 成長速度の振動 / 界面カイネティクス / 生体高分子 / 反射型干渉顕微鏡 / 固液界面

Outline of Final Research Achievements

Growth experiments of an ice crystal in a supercooled solution of antifreeze protein were carried out and separately measured the growth rates for the basal and prism faces. We clarified that the growth of basal faces was enhanced by the effect of antifreeze protein, while the growth of prism faces was suppressed. It means that the effects of antifreeze proteins for ice crystal growth were different for every crystallographic faces.
The growth promotion of basal face was explained by a new model that the adsorbed antifreeze protein molecules on the edges of growth steps may work as the new sources to generate new growth steps on the basal faces. We clarified that the mechanism for the freezing inhibition of living organisms under the sub-zero temperature environments was compatibly explained on the basis of the anisotropic functions of antifreeze proteins for ice crystal growth.

Research Products

• [Int'l Joint Research] Lobachevsky St. Univ. Nizhny Novgorod(ロシア連邦)
• [Int'l Joint Research] Alfred Wegener Institute(ドイツ)
• [Journal Article] Growth of Ice Crystals in the Presence of Type III Antifreeze Protein (2018)
  • Author(s): Dmitry A. Vorontsov, Gen Sazaki, Evgeniia K. Titaeva, Ekaterina L. Kim, Maddalena Bayer-Giraldi, and Yoshinori Furukawa
  • Journal Title: Cryst. Growth. Des. Volume: 18 Issue: 4 Pages: 2563-2571
  • DOI: 10.1021/acs.cgd.8b00172
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Temperature Dependence of the Growth Kinetics of Elementary Spiral Steps on Ice Basal Faces Grown from Water Vapor (2018)
  • Author(s): Inomata Masahiro、Murata Ken-ichiro、Asakawa Harutoshi、Nagashima Ken、Nakatsubo Shunichi、Furukawa Yoshinori、Sazaki Gen
  • Journal Title: Crystal Growth & Design Volume: 18 Issue: 2 Pages: 786-793
  • DOI: 10.1021/acs.cgd.7b01251
  • Peer Reviewed
• [Journal Article] Oscillations and accelerations of ice crystal growth rates in microgravity in presence of antifreeze glycoprotein impurity in supercooled water (2017)
  • Author(s): Yoshinori Furukawa, Ken Nagashima, Shun-ichi Nakatsubo, Izumi Yoshizaki, Haruka Tamaru, Taro Shimaoka, Takehiko Sone, Etsuro Yokoyama, Salvador Zepeda, Takanori Terasawa, Harutoshi Asakawa, Ken-ichiro Murata and Gen Sazaki
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 43157-43157
  • DOI: 10.1038/srep43157
  • NAID: 120006305557
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A new thermodynamic origin of surface melting on ice crystals (2016)
  • Author(s): K. Murata, H. Asakawa, K. Nagashima, Y. Furukawa
  • Journal Title: Proc. Nat. Acad. Sci. USA. Volume: 113 Issue: 44
  • DOI: 10.1073/pnas.1608888113
  • NAID: 130005974889
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] In-situ observation of crystal surfaces by optical microscopy (2016)
  • Author(s): Sazaki, Gen; Nagashima, Ken; Murata, Ken-ichiro; Furukawa, Yoshinori
  • Journal Title: Progress in Crystal Growth and Characterization of Materials Volume: 62 Pages: 408-412
  • NAID: 120006470957
  • Peer Reviewed / Open Access
• [Journal Article] Two types of quasi-liquid layers on ice crystals are formed kineticcally (2016)
  • Author(s): Harutoshi. Asakawa, Gen. Sazaki, Ken. Nagashima, S. Nakatsubo, Y. Furukawa
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 113 Issue: 7 Pages: 1749-1753
  • DOI: 10.1073/pnas.1521607113
  • Peer Reviewed / Open Access
• [Journal Article] Direct observation of bunching of elementary steps on protein crystals under forced flow conditions: (2015)
  • Author(s): G. Sazaki, G. Dai,
  • Journal Title: Theoretical & Applied Mechanics Letters Volume: 5 Issue: 4 Pages: 173-176
  • DOI: 10.1016/j.taml.2015.05.002
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Antifreeze effect of carboxylated e-poly-L-lysine on the growth kinetics of ice crystals (2014)
  • Author(s): D. A. Vorontsov, G. Sazaki, S. H. Hyon, K. Matsumura, Y. Furukawa
  • Journal Title: J. Phys. Chem. B Volume: 118 Issue: 34 Pages: 10240-10249
  • DOI: 10.1021/jp507697q
  • Peer Reviewed
• [Journal Article] Crystal Growth Experiments of Ice in Kibo of ISS (2014)
  • Author(s): Y. Furukawa, E. Yokoyama, I. Yoshizaki, H. Tamaru, T. Shimaoka, T. Sone,
  • Journal Title: Int. J. Microgravity Sci. Appl. Volume: 31 Pages: 93-99
  • NAID: 130007842472
  • Peer Reviewed / Open Access
• [Journal Article] 氷ベーサル面上での形態が異なる2種類の擬似液体層の生成過程 (2014)
  • Author(s): 佐崎元, 麻川明俊, 長嶋剣, 中坪俊一, 古川義純
  • Journal Title: Int. J. Microgravity Sci. Appl. Volume: 31 Pages: 100-106
  • NAID: 130007842470
  • Peer Reviewed / Open Access
• [Presentation] Anisotropic impurity effect for ice crystal growth in supercooled water (2018)
  • Author(s): FURUKAWA, Yoshinori, NAGASHIMA, Ken, NAKATSUBO, Shun-ichi, MURATA, Ken-ichiro, SAZAKI, Gen, ZEPEDA, Salvador, YOKOYAMA, Etsuro
  • Organizer: 14th International Conference on the Physics and Chemistry of Ice (PCI-2018)
  • Int'l Joint Research / Invited
• [Presentation] Crystallization of ice in AFP-III solutions (2018)
  • Author(s): VORONTSOV, Dmitry; SAZAKI, Gen; FURUKAWA Yoshinori; NAGASHIMA, Ken; MURATA, Ken-ichiro
  • Organizer: 14th International Conference on the Physics and Chemistry of Ice (PCI-2018)
  • Int'l Joint Research
• [Presentation] Temperature dependence of the growth kinetics of elementary spiral steps on ice basal faces grown from water vapor (2018)
  • Author(s): INOMATA, Masahiro; MURATA, Ken-ichiro; ASAKAWA, Harutoshi; NAGASHIMA, Ken; NAKATSUBO, Shunichi; FURUKAWA, Yoshinori; SAZAKI, Gen
  • Organizer: 14th International Conference on the Physics and Chemistry of Ice (PCI-2018)
  • Int'l Joint Research
• [Presentation] Hydrochloric acid droplets on ice crystal surfaces embedded in ice by vapor growth (2018)
  • Author(s): NAGASHIMA, Ken; SAZAKI, Gen; HAMA, Tetsuya; MURATA, Ken-ichiro; FURUKAWA, Yoshinori
  • Organizer: 14th International Conference on the Physics and Chemistry of Ice (PCI-2018)
  • Int'l Joint Research
• [Presentation] Time dependent change of tip velocities of a dendritic ice crystal in growing from supercooled water (2018)
  • Author(s): YOKOYAMA, Etsuro; FURUKAWA, Yoshinori 2
  • Organizer: 14th International Conference on the Physics and Chemistry of Ice (PCI-2018)
  • Int'l Joint Research
• [Presentation] Ice-binding proteins from a sea-ice diatom, their effect on ice growth and physical properties (2018)
  • Author(s): BAYER-GIRALDI, Maddalena; SAZAKI, Gen; AZUMA, Nobuhiko; TAKATA, Morimasa; KIPFSTUHL, Sepp; KONDO, Hidemasa; FURUKAWA, Yoshinori
  • Organizer: 14th International Conference on the Physics and Chemistry of Ice (PCI-2018)
  • Int'l Joint Research
• [Presentation] Anisotropic functions of antifreeze glycoproteins (2017)
  • Author(s): Yoshinori Furukawa
  • Organizer: 3rd Ice Binding Proteins Conference (IBP 3)
  • Int'l Joint Research / Invited
• [Presentation] 生体の凍結抑制機構と氷結晶の形 (2017)
  • Author(s): 古川義純; 長嶋剣; 中坪俊一; 田丸春香; 吉崎泉; 島岡太郎; 曽根 武彦
  • Organizer: 第８４回形の科学会シンポジウム
• [Presentation] Effect of type III antifreeze protein on the morphology and growth kinetics of ice crystals (2016)
  • Author(s): D. A. Vorontsov, G. Sazaki, K. Nagashima, K. Murata, Y. Furukawa
  • Organizer: The 18th International Conference on Crystal Growth and Epitaxy
  • Place of Presentation: Nagoya Congress Center, Nagoya
  • Year and Date: 2016-08-07
  • Int'l Joint Research
• [Presentation] Oscillatory Growth of Ice Crystal Observed in Supercooled Water Containing Antifreeze Glycoprotein (2016)
  • Author(s): Y. Furukawa, K. Nagashima, S. Nakatsubo, H. Tamaru, I. Yoshizaki, T. Shimaoka, T. Sone, E. Yokoyama, T. Maki, A. Yamamoto, H. Asakawa, K. Murata, G. Sazaki
  • Organizer: The 18th International Conference on Crystal Growth and Epitaxy
  • Place of Presentation: Nagoya Congress Center, Nagoya
  • Year and Date: 2016-08-07
  • Int'l Joint Research
• [Presentation] 宇宙実験での氷の結晶成長ー国際宇宙ステーションでの実験成果の概要ー (2015)
  • Author(s): 古川義純
  • Organizer: 第80回形の科学会シンポジウム
  • Place of Presentation: 東京電機大学、埼玉県比企郡鳩山町石坂
  • Year and Date: 2015-11-21
• [Presentation] 不凍糖タンパク質が制御する氷の振動成長 (2015)
  • Author(s): 古川義純，長嶋剣，横山悦郎，田丸晴香，吉崎泉，島岡太郎
  • Organizer: 第45会結晶成長国内会議
  • Place of Presentation: 北海道大学、北海道札幌市
  • Year and Date: 2015-10-19
  • Invited
• [Presentation] Oscillatory Growth of Ice Crystal Observed under Microgravity Condition in ISS-Kibo (2015)
  • Author(s): Y. Furukawa, K. Nagashima, S. Nakatsubo, I. Yoshizaki, T. Shimaoka, H. Tamaru, T. Sone, E. Yokoyama, T. Maki, A. Yamamoto
  • Organizer: The 22nd ELGRA Symposium and General Assembly
  • Place of Presentation: Corfu Chandres Villa & Hotel, Corfu, Greece
  • Year and Date: 2015-09-29
  • Int'l Joint Research
• [Presentation] 氷の自励振動成長―Ice Crystal 2 実験の成果― (2014)
  • Author(s): 古川義純，長嶋剣，麻川明俊，中坪俊一，村田憲一郎，佐崎元，吉崎泉，田丸晴香，島岡太郎，曽根武彦，真木孝雄，山本明日佳
  • Organizer: 第28回日本マイクログラビティ応用学会
  • Place of Presentation: イーグレ姫路、兵庫県姫路市
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 氷結晶の自励振動成長－国際宇宙ステーション実験の概要 (2014)
  • Author(s): 古川義純、長嶋剣、麻川明俊、中坪俊一、村田憲一郎、佐崎元、吉崎泉、田丸晴香、島岡太郎、曽根武彦、真木孝雄、山本明日佳
  • Organizer: 第44回結晶成長国内会議
  • Place of Presentation: 学習院大学百周年記念会館、東京都豊島区
  • Year and Date: 2014-11-06 – 2014-11-08
• [Presentation] Self-Oscillatry growth of ice-ice crystal 2 experiments carried out in "Kibo" of ISS- (2014)
  • Author(s): Y. Furukawa, K. Nagashima, E. Yokoyama, I. Yoshizaki, H. Tamaru, T. Shimaoka, T. Sone,
  • Organizer: 10th Asian Microgravity Symposium-2014
  • Place of Presentation: President Hotel, Seol, Korea
  • Year and Date: 2014-10-28 – 2014-10-31
• [Presentation] Oscillatory growth of ice crystal induced by effect of antifreeze glycoprotein (2014)
  • Author(s): Y. Furukawa
  • Organizer: FMSP Tutorial Symposium, Symposium on Mathematics for Various Discipline 13, “Mathematical Aspects of Surface and Interface Dynamics VIII” University of Tokyo
  • Place of Presentation: 東京大学、駒場、東京都目黒区
  • Year and Date: 2014-10-22 – 2014-10-24
  • Invited
• [Presentation] Oscillatory growth of ice crystal in the solution of antifreeze glycoprotein (2014)
  • Author(s): Y. Furukawa
  • Organizer: 2nd Ice Binding Protein Conference (IBP-2)
  • Place of Presentation: 北大学術交流会館、札幌市北区
  • Year and Date: 2014-08-04 – 2014-08-07
  • Invited
• [Presentation] Self-Oscillatory Growth of Ice Crystal-Microgravity Experiments in Kibo of ISS- (2014)
  • Author(s): Y. Furukawa
  • Organizer: 24th AACGE Western Section Conference on Crystal growth & Epitaxy
  • Place of Presentation: Stanford Siera Lodge, Fallen-Leaf Lake, USA
  • Year and Date: 2014-06-08 – 2014-06-11
  • Invited
• [Remarks] 流氷の海に住む魚はなぜ凍死しない？ 宇宙実験で解き明かす
  • URL: https://academist-cf.com/journal/?p=4410
• [Remarks] 氷点下でも氷が凍らないのはなぜ？宇宙ステーションで結晶成長を実験
  • URL: http://www.lowtem.hokudai.ac.jp/frontiers/case06/