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Development for an ultrasensitive and non-labeled detection method of non-fluorescent molecules in liquid solutions

Research Project

Project/Area Number 26288068
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Analytical chemistry
Research InstitutionKyushu University

Principal Investigator

Harata Akira  九州大学, 総合理工学研究院, 教授 (90222231)

Co-Investigator(Renkei-kenkyūsha) ISODA Miki  九州大学, 大学院総合理工学府, 技術専門職員 (70467885)
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
Keywords超高感度分離検出 / 非蛍光性化学種 / 紫外レーザー分光 / 光熱変換 / ヘテロダイン検出 / 超高速液体クロマトグラフィー / マイクロチップ / 溶液中化学種 / 2色2光子励起 / 顕微イメージング / ミクロ液体クロマトグラフィー
Outline of Final Research Achievements

To enhance versatility of photothermal heterodyne interferometer, a highly sensitive and direct detection method for biologically and environmentally important molecules in liquid solutions with no labeling pretreatment, deep ultraviolet laser at the wavelength of 213 nm is equipped for excitation and the performance of the detection system is evaluated in comparison with a thermal lens detection system. The performance for separation/detection analysis is evaluated in combination with a micro-HPLC and a micro-chip flow device. Non- and less-fluorescent chemicals having no visible light absorption are target species of detection for which fluorometry of the ultrahigh sensitive detection method is quite powerless unless troublesome labelling pretreatment.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (25 results)

All 2018 2017 2016 2015 2014 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (20 results) (of which Int'l Joint Research: 4 results,  Invited: 2 results) Remarks (3 results)

  • [Journal Article] Photoionization of environmentally polluting aromatic chlorides and nitrides on the water surface by laser and synchrotron radiations2017

    • Author(s)
      Miki Sato, Yuki Maeda, Tohsio Ishioka, Akira Harata
    • Journal Title

      Analyst

      Volume: 142 Issue: 23 Pages: 4560-4569

    • DOI

      10.1039/c7an01311f

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 環境・生体関連分子計測法の開発2014

    • Author(s)
      原田 明
    • Journal Title

      グリーンアジア国際戦略プログラム NewsLetter

      Volume: 3 Pages: 9-9

    • Related Report
      2014 Annual Research Report
    • Open Access
  • [Presentation] 高感度分離検出のためのミクロ高速液体クロマトグラフィー/光熱変換ヘテロダイン干渉法の開発2018

    • Author(s)
      礒田 美紀、福間 誠、原田 明
    • Organizer
      九州大学技術発表会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ミクロ高速液体クロマトグラフィー/光熱変換ヘテロダイン干渉法によるニトロ多環芳香族炭化水素の高感度分離検出2017

    • Author(s)
      礒田 美紀、原田 明
    • Organizer
      総合技術研究会2017東京大学
    • Place of Presentation
      東京大学、東京
    • Year and Date
      2017-03-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] HPLC/紫外励起顕微光熱変換ヘテロダイン干渉検出法を用いたニトロ多環芳香族炭化水素の高感度分析法開発2017

    • Author(s)
      安倍 聡彦、 礒田 美紀、 原田 明
    • Organizer
      第54回化学関連支部合同九州大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] セミミクロHPLC・深紫外レーザー励起熱レンズ分光法による無標識アミノ酸の分離・検出2017

    • Author(s)
      原田 明、當房 実咲、礒田 美紀
    • Organizer
      日本分析化学会66年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Development of an analytical method of nitropolycyclic aromatic hydrocarbons using ultraviolet-excitation micro-photothermal heterodyne-interferometer2017

    • Author(s)
      Toshihiko Abe, Miki Isoda, Akira Harata
    • Organizer
      第38回超音波エレクトロニクスの基礎と応用に関するシンポジウム
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] HPLC用紫外光励起光熱変換ヘテロダイン干渉検出器の開発2016

    • Author(s)
      原田 明、福間 誠、青木 開、礒田 美紀
    • Organizer
      日本分析化学会第65年会
    • Place of Presentation
      北海道大学、札幌
    • Year and Date
      2016-09-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 深紫外励起熱レンズ分光法による無標識アミノ酸の分離・高感度検出2016

    • Author(s)
      當房 実咲、礒田 美紀、原田 明
    • Organizer
      第53回化学関連支部合同九州大会
    • Place of Presentation
      北九州国際会議場、小倉
    • Related Report
      2016 Annual Research Report
  • [Presentation] Development of deep ultraviolet-excitation photothermal heterodyne-intereferometer combined with capillary HPLC for label-free analysis of biologically-relevant molecules2015

    • Author(s)
      Kai Aoki, Miki Isoda, and Akira Harata
    • Organizer
      2015 International Chemical Congress of Pacific Basin Societies (Pacifichem 2015)
    • Place of Presentation
      Honolulu, U.S.A
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of photothermal heterodyne-interferometric detection method as micro-HPLC detector2015

    • Author(s)
      Miki Isoda, Makoto Fukuma, Akira Harata
    • Organizer
      2015 International Chemical Congress of Pacific Basin Societies (Pacifichem 2015)
    • Place of Presentation
      Honolulu, U.S.A
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 光ビーム照射で生じる諸現象の高感度・高精度計測に基づいた分子情報の未開拓領域探索法の探索2015

    • Author(s)
      原田 明
    • Organizer
      2015年度九州支部講演会 (2015年度 九州分析化学会賞受講演)
    • Place of Presentation
      京セラ株式会社鹿児島国分工場
    • Year and Date
      2015-11-20
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Deep Ultraviolet-Excitation Photothermal Heterodyne-Interferometer Combined with micro-HPLC2015

    • Author(s)
      Kai Aoki, Miki Isoda, and Akira Harata
    • Organizer
      第36回超音波エレクトロニクスの基礎と応用に関するシンポジウム
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2015-11-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 深紫外励起光熱変換ヘテロダイン干渉検出を用いた無標識アミノ酸の微量・高感度分離分析法の開発2015

    • Author(s)
      青木 開、礒田 美紀、原田 明
    • Organizer
      第52回化学関連支部合同九州大会 (ポスター賞)
    • Place of Presentation
      北九州国際会議場
    • Year and Date
      2015-06-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] evelopment of a Microscopic Photothermal Heterodyne-interferometric Detection Method Combined with a Micro-HPLC for Highly Sensitive Analysis2015

    • Author(s)
      Makoto Fukuma, Miki Isoda, Akira Harata
    • Organizer
      The First Joint Symposium of Kyushu University and Yonsei University on Materials Science and Chemical Engineering
    • Place of Presentation
      福岡(九州大学筑紫キャンパス)
    • Year and Date
      2015-02-05 – 2015-02-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] Development of microscopic photothermal heterodyne-interferometric detection method combined with a micro-HPLC for highly sensitive analysis2014

    • Author(s)
      福間 誠、礒田 美紀、原田 明
    • Organizer
      第36回超音波エレクトロニクスの基礎と応用に関するシンポジウム
    • Place of Presentation
      お茶の水(明治大学アカデミーコモン)
    • Year and Date
      2014-12-03 – 2014-12-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] Performance Estimation of Photothermal Contrast-Applied Microscope2014

    • Author(s)
      眞子 翠、原田 明
    • Organizer
      第36回超音波エレクトロニクスの基礎と応用に関するシンポジウム
    • Place of Presentation
      お茶の水(明治大学アカデミーコモン)
    • Year and Date
      2014-12-03 – 2014-12-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] Development of microscopic photothermal heterodyne-interferometric detection method combined with a micro-HPLC for highly sensitive analysis of bio-related materials2014

    • Author(s)
      Makoto Fukuma, Miki Isoda, Akira Harata
    • Organizer
      The 16th Cross Straits Symposium on Energy, Environmental Science and Technology
    • Place of Presentation
      Korea(Pusan National Univ.)
    • Year and Date
      2014-11-13 – 2014-11-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] Development of a Photothermal Contrast Applied Microscope2014

    • Author(s)
      Midori Manago, Akira Harata
    • Organizer
      The 16th Cross Straits Symposium on Energy, Environmental Science and Technology
    • Place of Presentation
      Korea(Pusan National Univ.)
    • Year and Date
      2014-11-13 – 2014-11-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] 溶液中非蛍光性化学種の無標識超高感度分離検出最終形2014

    • Author(s)
      原田 明、福間 誠、礒田 美紀
    • Organizer
      日本分析化学会第63年会
    • Place of Presentation
      東広島市(広島大学)
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] micro-HPLC/ヘテロダイン干渉検出法を用いたアミノ酸の高感度分離および検出系の開発2014

    • Author(s)
      青木 開、原田 明
    • Organizer
      第32回九州分析化学若手の会夏季セミナー
    • Place of Presentation
      北九州(北九州かんぽの宿)
    • Year and Date
      2014-07-25 – 2014-07-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 光熱変換コントラストを用いた顕微鏡の開発2014

    • Author(s)
      眞子 翠、野崎 恭平、原田 明
    • Organizer
      第51回化学関連支部合同九州大会
    • Place of Presentation
      小倉(北九州国際会議場)
    • Year and Date
      2014-06-28
    • Related Report
      2014 Annual Research Report
  • [Remarks] 九州大学大学院総合理工学府原田・薮下研究室

    • URL

      http://www.mm.kyushu-u.ac.jp/lab_07/

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Remarks] 「原田 明」教育研究関連のデータベース

    • URL

      http://www.mm.kyushu-u.ac.jp/lab_07/members/harata/AHDB_01.html

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report 2015 Annual Research Report 2014 Annual Research Report
  • [Remarks] 九州大学大学院総合理工学府物質理工学専攻 原田・薮下研究室

    • URL

      http://www.mm.kyushu-u.ac.jp/lab_07/

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2019-03-29  

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