Chemically modified DNAs cotrolled by triplex forming oligodeoxynucleotides
| Project/Area Number |
26288075
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
Seio Kohji 東京工業大学, 生命理工学院, 准教授 (20313356)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
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| Keywords | 三重鎖DNA / 人工遺伝子 / 蛍光核酸 / DNA三重鎖 / 合成生物学 / 核酸 / 有機化学 / 三重鎖核酸 / 三重鎖形成核酸 / 人工塩基 / 人工塩基対 |
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| Outline of Final Research Achievements |
We developed artificial DNAs whose function could be controlled by triplex formation. First we developed artificial genes incorporating pseudouracil and pseudoisocytosine which had hydrogen bond donor in their minor groove sides. The modified DNAs can be transcribed by T7-RNA polymerase and the transcrption was inhibited by the addition of triplex forming oligonucleotides incorporating artificial bases which can form hydrogen bond with pseudouracil or pseudoisocytosine. We also developed fluorescent DNAs having aromatic groups in their minor groove. The spectroscopic analyses revealed that the fluorescence of the DNAs can be enhanced by addition of triplex forming oligonucleotides.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Enzymatic synthesis and reverse transcription of RNAs incorporating 2 -O-carbamoyl uridine triphosphate2016
Author(s)
Masaki Y, Ito H, Oda Y, Yamazaki K, Tago N, Ohno K, Ishii N, Tsunoda H, Kanamori T, Ohkubo A, Sekine M, Seio K.
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Journal Title
Chemical Communications
Volume: 52
Issue: 87
Pages: 12889-12892
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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