| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Outline of Final Research Achievements |
Understanding tumor microenvironment is crucial for analyzing response of solid tumor to therapy and in the augmentation of that response. Oxygen status, which fluctuates quickly, is one of the most important components that should be analyzed, because oxygen makes a profound impact on treatment resistance, especially against radiotherapy. Therefore, tractable technology for the oxygen status has been imperative.
We successfully monitored oxygen fluctuation in cell nucleus and tumor tissue directly. Ruthenium complex with Hoechst unit (Ru-Hoechst) and ruthenium complex-labeled oligonucleotides (DRC) with pyrene and nitroimidazole units were synthesized and we evaluated their properties. These probe showed oxygen-dependent phosphorescence at cellular nucleus and tumor tissue and therefore, we could monitor the oxygen levels. These probes will be applicable to monitoring of oxygen levels and assessment of response of tumor tissue against radiation on the basis of oxygen quantification.
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