| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2014: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Outline of Final Research Achievements |
Amyloidosis has been a serious problem in the aging society. To remedy amyloidosis, the most effective way is to hydrolyze amyloid fibrils. We thus tried to design short peptides having hydrolysis activity against amyloid fibrils. As a strategy for the design, we made the peptide to have the catalytic triad composed of histidine, aspartic acid and serine. Based on the strategy, we synthesized 9 peptides named me1-9. It has been shown that me5 takes the alpha-helical conformation and can hydrolyze amyloid fibrils. We tried to hydrolyze variety of amyloids: beta lactoglobulin, insulin, amyloid beta40, amyloid beta42 and beta 2 microglobulin. It is concluded that the peptide me5 can be regarded as a hydrolase which is capable of hydrolyzing the amyloid fibrils.
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