Development of supramolecular construction techniques of proteins and their functionalization using domain swapping

• Project/Area Number: 26288080
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: HIROTA Shun 奈良先端科学技術大学院大学, 物質創成科学研究科, 教授 (90283457)
• Co-Investigator(Renkei-kenkyūsha): HIGUCHI Yoshiki 兵庫県立大学, 大学院生命理学研究科, 教授 (90183574) ; NAGAO Satoshi 奈良先端科学技術大学院大学, 物質創成科学研究科, 助教 (30452535) ; YAMANAKA Masaru 奈良先端科学技術大学院大学, 物質創成科学研究科, 助教 (60632825)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000); Fiscal Year 2016: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: タンパク質 / 生体分子 / ナノバイオ / 超分子化学 / 多量体 / 立体構造 / ドメインスワッピング / 蛋白質 / 超分子

Outline of Final Research Achievements

A certain amount of molten globule state oligomers of horse cyt c converted to domain-swapped oligomers by folding from the acid molten globule state to neutral pH state under high protein concentrations. Domain-swapped dimer stability of horse cyt c was less affected by the Met80 coordination to the heme. Two domain-swapped structures of thermostable cyt c552 were detected for its hinge loop mutant. Domain swapping was shown to also occur for thermostable cyt c552 when expressed in E. coli cells. A tetramer of cyt c′ was constructed with one domain-swapped dimer and two monomers. An artificial myoglobin heterodimer with two different heme active sites was constructed based on domain swapping.

Research Products

• [Journal Article] Formation and Carbon Monoxide-Dependent Dissociation of Allochromatium vinosum Cytochrome c' Oligomers Using Domain-Swapped Dimers (2017)
  • Author(s): M. Yamanaka, M. Hoshizumi, S. Nagao, R. Nakayama, N. Shibata, Y. Higuchi, and S. Hirota
  • Journal Title: Protein Science Volume: 26 Issue: 3 Pages: 464-474
  • DOI: 10.1002/pro.3090
  • NAID: 120006334944
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Improved Stopped-Flow Time-Resolved Resonance Raman Spectroscopy Device for Studying Enzymatic Reactions (2017)
  • Author(s): S. Yanagisawa, M. S. Deshpande, S. Hirota, T. Nakagawa, and T. Ogura
  • Journal Title: Journal of Raman Spectroscopy Volume: 48 Issue: 5 Pages: 680-685
  • DOI: 10.1002/jrs.5100
  • NAID: 120006334945
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effect of Methionine80 Heme Coordination on Domain Swapping of Cytochrome c (2017)
  • Author(s): S. Hirota, N. Yamashiro, Z. Wang, and S. Nagao
  • Journal Title: Journal of Biological Inorganic Chemistry Volume: 22 Issue: 5 Pages: 705-712
  • DOI: 10.1007/s00775-017-1446-3
  • NAID: 120006334946
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A Single Spherical Assembly of Protein Amyloid Fibrils Formed by Laser Trapping (2017)
  • Author(s): K. Yuyama, M. Ueda, S. Nagao, S. Hirota, T. Sugiyama, and H. Masuhara
  • Journal Title: Angewandte Chemie Interanational Edition Volume: 56 Issue: 24 Pages: 6739-6743
  • DOI: 10.1002/anie.201702352
  • NAID: 120006473600
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Characterization of Cytochrome c Membrane-Binding Site Using Cardiolipin-Containing Bicelles with NMR (2016)
  • Author(s): H. Kobayashi, S. Nagao, and S. Hirota
  • Journal Title: Angewandte Chemie International Edition Volume: 55 Issue: 45 Pages: 14019-14022
  • DOI: 10.1002/anie.201607419
  • NAID: 120005850423
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Domain swapping oligomerization of thermostable c-type cytochrome in E. coli cells (2016)
  • Author(s): Yugo Hayashi, Masaru Yamanaka, Satoshi Nagao, Hirofumi Komori, Yoshiki Higuchi, Shun Hirota
  • Journal Title: Scientific reports Volume: 6 Issue: 1 Pages: 19334-19334
  • DOI: 10.1038/srep19334
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Domain-swapped Cytochrome cb562 Dimer and Its Nanocage Encapsulating a Zn-SO4 Cluster in the Internal Cavity (2015)
  • Author(s): Takaaki Miyamoto, Mai Kuribayashi, Satoshi Nagao, Yasuhito Shomura, Yoshiki Higuchi, Shun Hirota
  • Journal Title: Chemical Science Volume: 6 Issue: 12 Pages: 7336-7342
  • DOI: 10.1039/c5sc02428e
  • NAID: 120006334940
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Oligomerization Enhancement and Two Domain Swapping Mode Detection for Thermostable Cytochrome c-552 via the Elongation of the Major Hinge Loop (2015)
  • Author(s): C. Ren, S. Nagao, M. Yamanaka, H. Komori, Y. Shomura, Y. Higuchi and S. Hirota
  • Journal Title: Mol. Biosyst. Volume: 11(12) Issue: 12 Pages: 218-221
  • DOI: 10.1039/c5mb00545k
  • NAID: 120006334941
  • Peer Reviewed
• [Journal Article] Rational design of heterodimeric protein using domain swapping for myoglobin (2015)
  • Author(s): Ying-Wu Lin, Satoshi Nagao, Mohan Zhang, Yasuhito Shomura, Yoshiki Higuchi, Shun Hirota
  • Journal Title: Angewandte Chemie International Edition Volume: 54 Issue: 2 Pages: 511-515
  • DOI: 10.1002/anie.201409267
  • NAID: 120005716859
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Carbon monoxide binding properties of domain-swapped dimeric myoglobin (2015)
  • Author(s): Satoshi Nagao, Haruto Ishikawa, Takuya Yamada, Yasuhisa Mizutani, Shun Hirota
  • Journal Title: Journal of biological inorganic chemistry Volume: 20 Issue: 3 Pages: 523-530
  • DOI: 10.1007/s00775-014-1236-0
  • NAID: 120005716870
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Domain-swapped dimer of Pseudomonas aeruginosa cytochrome c551: Structural insights into domain swapping of cytochrome c family proteins (2015)
  • Author(s): Satoshi Nagao, Mariko Ueda, Hisao Osuka, Hirofumi Komori, Hironari Kamikubo, Mikio Kataoka, Yoshiki Higuchi, Shun Hirota
  • Journal Title: PLoS One Volume: 10 Issue: 4 Pages: e0123653-e0123653
  • DOI: 10.1371/journal.pone.0123653
  • NAID: 120005716860
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Formation of Domain-Swapped Oligomer of Cytochrome c from Its Molten Globule State Oligomer (2014)
  • Author(s): Megha Deshpande, Partha Parui, Hironari Kamikubo, Masaru Yamanaka, Satoshi Nagao, Hirofumi Komori, Mikio Kataoka, Yoshiki Higuchi, Shun Hirota
  • Journal Title: Biochemistry Volume: 53(28) Issue: 28 Pages: 4696-4703
  • DOI: 10.1021/bi500497s
  • Peer Reviewed
• [Journal Article] Self-Oxidation of Cytochrome c at Methionine80 with Molecular Oxygen Induced by Cleavage of the Met－Heme Iron Bond (2014)
  • Author(s): Z. Wang, Y. Ando, A. D. Nugraheni, C. Ren, S. Nagao, S. Hirota
  • Journal Title: Molecular BioSystems Volume: 10 Issue: 12 Pages: 3130-3137
  • DOI: 10.1039/c4mb00285g
  • NAID: 120005716873
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] DNA Cleavage by Oxymyoglobin and Cysteine-Introduced Metmyoglobin (2014)
  • Author(s): M. S. Deshpande, S. Junedi, H. Prakash, S. Nagao, M. Yamanaka, S. Hirota
  • Journal Title: Chemical Communications Volume: 50 Issue: 95 Pages: 15034-15036
  • DOI: 10.1039/c4cc06617k
  • NAID: 120005716862
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Control of Transition between Ni-C and Ni-SIa States in Catalytic Cycle of [NiFe] Hydrogenase by Redox State of its Proximal Fe-S Cluster (2014)
  • Author(s): H. Tai, K. Nishikawa, M. Suzuki, Y. Higuchi, and S. Hirota
  • Journal Title: Angew. Chem. Int. Ed. Volume: 53 Issue: 50 Pages: 13817-13820
  • DOI: 10.1002/anie.201408552
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Change in Structure and Ligand Binding Properties of Hyperstable Cytochrome c555 from Aquifex aeolicus by Domain Swapping (2014)
  • Author(s): M. Yamanaka, S. Nagao, H. Komori, Y. Higuchi and S. Hirota
  • Journal Title: Protein Science Volume: 24 Issue: 3 Pages: 366-375
  • DOI: 10.1002/pro.2627
  • NAID: 120005716876
  • Peer Reviewed
• [Presentation] Design of Domain-swapped Myoglobin Dimer (2017)
  • Author(s): Satoshi Nagao, Ayaka Suda, Hisashi Kobayashi, Naoki Shibata, Yoshiki Higuchi, and Shun Hirota
  • Organizer: 97th Chemical Society of Japan Annual Meeting
  • Place of Presentation: 慶応義塾大学(神奈川県横浜市)
  • Year and Date: 2017-03-16
• [Presentation] Rhodopseudomonas palustris由来シトクロムc′の多量化 (2017)
  • Author(s): 久保田真史, 宮本昂明、廣田俊
  • Organizer: 日本化学会第97春季年会
  • Place of Presentation: 慶応義塾大学(神奈川県横浜市)
  • Year and Date: 2017-03-16
• [Presentation] Laser Trapping Dynamics of Amyloid Fibril Formation of Cytochrome c Monomer and Dimers (2017)
  • Author(s): Chieh-Ju Chang, Yugo Hayashi, Shun Hirota, Ken-ichi Yuyama, Teruki Sugiyama, and Hirotashi Masuhara
  • Organizer: 97th Chemical Society of Japan Annual Meeting
  • Place of Presentation: 慶応義塾大学(神奈川県横浜市)
  • Year and Date: 2017-03-16
• [Presentation] Effect of Rare Codons on Hydrogenobacter thermophiles cyt c552 Oligomerization in E. coli Cells (2017)
  • Author(s): Hongxu Yang, Masaru Yamanaka, Satoshi Nagao, and Shun Hirota
  • Organizer: 97th Chemical Society of Japan Annual Meeting
  • Place of Presentation: 慶応義塾大学(神奈川県横浜市)
  • Year and Date: 2017-03-16
• [Presentation] Formation of Cytochrome c Oligomers Using Domain-Swapped Dimers and Carbon Monoxide-Dependent Control of Oligomer Association/Dissociation (2017)
  • Author(s): Masaru Yamanaka, Ryoko Nakayama, Satoshi Nagao, Makoto Hoshizumi, Naoki Shibata, Yoshiki Higuchi, and Shun Hirota
  • Organizer: 97th Chemical Society of Japan Annual Meeting
  • Place of Presentation: 慶応義塾大学(神奈川県横浜市)
  • Year and Date: 2017-03-16
• [Presentation] Characterization of the Cytochrome c Membrane-binding Site Using Solution NMR (2017)
  • Author(s): Hisashi Kobayashi, Satoshi Nagao, and Shun Hirota
  • Organizer: 97th Chemical Society of Japan Annual Meeting
  • Place of Presentation: 慶応義塾大学(神奈川県横浜市)
  • Year and Date: 2017-03-16
• [Presentation] Protein Nanostructures Constructed by Fusing Two Oligomerization Domains with a 3-Helix Bundle Linker (2017)
  • Author(s): Takaaki Miyamoto and Shun Hirota
  • Organizer: 97th Chemical Society of Japan Annual Meeting
  • Place of Presentation: 慶応義塾大学(神奈川県横浜市)
  • Year and Date: 2017-03-16
• [Presentation] Construction of a Triangle-shaped Structure with a Unit Protein Based on Cytochrome c555 (2017)
  • Author(s): Akiya Oda, Ikki Ueda, Masaru Yamanaka, Satoshi Nagao, Naoki Shibata, Yoshiki Higuchi, and Shun Hirota
  • Organizer: 97th Chemical Society of Japan Annual Meeting
  • Place of Presentation: 慶応義塾大学(神奈川県横浜市)
  • Year and Date: 2017-03-16
• [Presentation] Cytochrome c: Domain Swapping, Self-Modification, and Molecular Recognition (2017)
  • Author(s): Shun Hirota, Satoshi Nagao, Masaru Yamanaka, and Yoshiki Higuchi
  • Organizer: 5th Symposium on Advanced Biological Inorganic Chemistry
  • Place of Presentation: Kolkata, India
  • Year and Date: 2017-01-07
  • Int'l Joint Research / Invited
• [Presentation] Construction of Heme Protein Supramolecules by Domain Swapping (2016)
  • Author(s): Shun Hirota, Satoshi Nagao, Masaru Yamanaka, and Yoshiki Higuchi
  • Organizer: 5th Symposium on Advanced Biological Inorganic Chemistry
  • Place of Presentation: Auckland, New Zealand
  • Year and Date: 2016-12-04
  • Int'l Joint Research / Invited
• [Presentation] Solution NMR Characterization of the Interaction between Cyt c and Cardiolipin-incorporated Bicelles (2016)
  • Author(s): Hisashi Kobayashi, Satoshi Nagao, and Shun Hirota
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: つくば国際会議場（茨城県つくば市）
  • Year and Date: 2016-11-25
• [Presentation] Analysis of Environment Dependent Dynamics of Cytochrome c on a Lipid Membrane by 2D Fluorescence Lifetime Correlation Spectroscopy (2016)
  • Author(s): Miyuki Sakaguchi，Masaru Yamanaka，Shun Hirota，Kunihiko Ishii，and Tahei Tahara
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: つくば国際会議場（茨城県つくば市）
  • Year and Date: 2016-11-25
• [Presentation] Formation of Myoglobin Dimers by Domain Swapping (2016)
  • Author(s): Satoshi Nagao, Ayaka Suda, Hisashi Kobayashi, Naoki Shibata, Yoshiki Higuchi, and Shun Hirota
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: つくば国際会議場（茨城県つくば市）
  • Year and Date: 2016-11-25
• [Presentation] Structure and Function of Heme Protein Oligomers Constructed by Domain Swapping (2016)
  • Author(s): Shun Hirota
  • Organizer: Japan-Korea-Taiwan Bioinorganic Chemistry Symposium
  • Place of Presentation: つくば国際会議場（茨城県つくば市）
  • Year and Date: 2016-09-29
  • Int'l Joint Research / Invited
• [Presentation] Application of Supramolecular Science for Drug Design (2016)
  • Author(s): Shun Hirota
  • Organizer: The Symposium of Annual Scientific Meeting 2016 of Indonesian Pharmacist Association
  • Place of Presentation: Jogjakarta, Indonesia
  • Year and Date: 2016-09-27
  • Int'l Joint Research / Invited
• [Presentation] 二次元蛍光寿命相関分光法によるウマシトクロムcのフォールディング機構の研究 (2016)
  • Author(s): 坂口美幸、山中優、廣田俊、石井邦彦、田原太平
  • Organizer: 第10回分子科学討論会
  • Place of Presentation: 神戸ファッションマート（兵庫県神戸市）
  • Year and Date: 2016-09-13
• [Presentation] 過酸との反応によるシトクロムcのMet80の酸化修飾 (2016)
  • Author(s): 松本順文、REN Chunguang、山中優、長尾聡、廣田俊
  • Organizer: 第10回バイオ関連化学シンポジウム
  • Place of Presentation: 石川県立音楽堂・もてなしドーム地下イベント広場（石川県金沢市）
  • Year and Date: 2016-09-07
• [Presentation] ミオグロビンのループ領域アミノ酸変異によるドメインスワップ二量体のデザイン (2016)
  • Author(s): 須田綾香、小林紀、長尾聡、柴田直樹、樋口芳樹、廣田俊
  • Organizer: 第10回バイオ関連化学シンポジウム
  • Place of Presentation: 石川県立音楽堂・もてなしドーム地下イベント広場（石川県金沢市）
  • Year and Date: 2016-09-07
• [Presentation] Domain Swapping in c-Type Cytochromes and Myoglobin (2016)
  • Author(s): Shun Hirota, Satoshi Nagao, Partha Pratim Parui, Megha Subhash Deshpande, Chunguang Ren, Yugo Hayashi, Takaaki Miyamoto, Yin-Wu Lin, and Yoshiki Higuchi
  • Organizer: 30th Anniversary Symposium of the Protein Society
  • Place of Presentation: Baltimore, USA
  • Year and Date: 2016-07-16
  • Int'l Joint Research
• [Presentation] Construction of Zn-SO4 Cluster-encapsulating Protein Nanocage by Domain Swapping (2016)
  • Author(s): Takaaki Miyamoto, Mai Kuribayashi, Satoshi Nagao, Yasuhito Shomura, Yoshiki Higuchi, and Shun Hirota
  • Organizer: 30th Anniversary Symposium of the Protein Society
  • Place of Presentation: Baltimore, USA
  • Year and Date: 2016-07-16
  • Int'l Joint Research
• [Presentation] Characterization of the Interaction between Cyt c and Cardiolipin-incorporated by Solution NMR (2016)
  • Author(s): Hisashi Kobayashi, Satoshi Nagao, and Shun Hirota
  • Organizer: 30th Anniversary Symposium of the Protein Society
  • Place of Presentation: Baltimore, USA
  • Year and Date: 2016-07-16
  • Int'l Joint Research
• [Presentation] Construction of Zn-Domain Swapping of c-Type Cytochromes and Myoglobin (2016)
  • Author(s): Shun Hirota
  • Organizer: Ninth International Conference on Porphyrins and Phthalocyanines
  • Place of Presentation: Nanjing, China
  • Year and Date: 2016-07-03
  • Int'l Joint Research / Invited
• [Presentation] バイセルを利用したシトクロムcの脂質膜結合部位のNMR研究 (2016)
  • Author(s): 小林紀、長尾聡、廣田俊
  • Organizer: 第43回生体分子科学討論会
  • Place of Presentation: 名古屋大学（愛知県名古屋市）
  • Year and Date: 2016-06-24
• [Presentation] 溶液NMRによるシトクロムcとカルジオリピン含有バイセルの相互作用解析 (2016)
  • Author(s): 小林紀、長尾聡、廣田俊
  • Organizer: 第26回金属の関与する生体関連反応シンポジウム
  • Place of Presentation: 北海道大学（北海道札幌市）
  • Year and Date: 2016-06-17
• [Presentation] 四量体シトクロムc’の作製とCO分子結合・解離によるサイズ制御 (2016)
  • Author(s): 山中優、星住誠人、長尾聡、廣田俊
  • Organizer: 第1６回日本蛋白質科学会年会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2016-06-07
• [Presentation] ドメインスワップしたシトクロムcb562二量体が形成するZn-SO4クラスター内包タンパク質ナノケージの結晶構造 (2016)
  • Author(s): 宮本昂明、長尾聡、庄村康人、樋口芳樹、廣田俊
  • Organizer: 第16回日本蛋白質科学会年会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2016-06-07
• [Presentation] Characterization of the Interaction of Cytochrome c with Cardiolipin-incorporated Lipid Bicelle (2016)
  • Author(s): Hisashi Kobayashi, Satoshi Nagao, Shun Hirota
  • Organizer: 96th Spring Conference of the Chemical Society of Japan
  • Place of Presentation: Doshisha University (Kyotanabe, Kyoto)
  • Year and Date: 2016-03-24
• [Presentation] Domain-swapped Cytochrome cb562 Dimer and Its Nanocage Encapsulating a Zn-SO4 Cluster in the Internal Cavity (2016)
  • Author(s): Takaaki Miyamoto, Mai Kuribayashi, Satoshi Nagao, Yasuhito Shomura, Yoshiki Higuchi, Shun Hirota
  • Organizer: 96th Spring Conference of the Chemical Society of Japan
  • Place of Presentation: Doshisha University (Kyotanabe, Kyoto)
  • Year and Date: 2016-03-24
• [Presentation] ドメインスワップ構造に基づいた安定なミオグロビン二量体のデザイン (2016)
  • Author(s): 須田綾香、小林紀、長尾聡、廣田俊
  • Organizer: 日本化学会第96春季年会
  • Place of Presentation: 同志社大学（京都府京田辺市）
  • Year and Date: 2016-03-24
• [Presentation] Construction of Cytochrome c' Tetramer and Its Size Control by CO Binding (2016)
  • Author(s): 山中優、星住誠人、廣田俊
  • Organizer: 日本化学会第96春季年会
  • Place of Presentation: 同志社大学（京都府京田辺市）
  • Year and Date: 2016-03-24
• [Presentation] Construction of Heme Protein Oligomers by Domain Swapping (2015)
  • Author(s): Shun Hirota
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2015 (Pacifichem 2015)
  • Place of Presentation: Hilton Hawaiian Village (Honolulu, USA)
  • Year and Date: 2015-12-15
  • Int'l Joint Research / Invited
• [Presentation] 好熱菌シトクロムc552のドメインスワップ多量体形成 (2015)
  • Author(s): 林有吾、山中優、長尾聡、小森博文、樋口芳樹、廣田俊
  • Organizer: 日本生物高分子学会2015年度大会
  • Place of Presentation: 香川大学（香川県高松市）
  • Year and Date: 2015-09-19
• [Presentation] ドメインスワップ構造に基づくミオグロビン二量体のデザイン (2015)
  • Author(s): 長尾聡、Ying-Wu Lin、Mohan Zhang、庄村康人、樋口芳樹、廣田俊
  • Organizer: 第9回バイオ関連化学シンポジウム
  • Place of Presentation: 熊本大学（熊本県熊本市）
  • Year and Date: 2015-09-10
• [Presentation] シトクロムcとカルジオリピン含有脂質膜の相互作用解析 (2015)
  • Author(s): 小林紀、長尾聡、廣田俊
  • Organizer: 第9回バイオ関連化学シンポジウム
  • Place of Presentation: 熊本大学（熊本県熊本市）
  • Year and Date: 2015-09-10
• [Presentation] ドメインスワッピングとは？：タンパク質の超分子化 (2015)
  • Author(s): 廣田俊
  • Organizer: 第28回生物無機化学夏季セミナー
  • Place of Presentation: 山喜旅館（静岡県伊東市）
  • Year and Date: 2015-08-28
  • Invited
• [Presentation] Spatio-Temporal Formation of Amyloid Aggregates by Laser Trapping of Domain-swapped Dimeric Cytochrome c (2015)
  • Author(s): Ken-ichi Yuyama, Mariko Ueda, Satoshi Nagao, Shun Hirota, Teruki Suigiyama, Hiroshi Masuhara
  • Organizer: Optical Trapping and Optical Micromanipulation XII, SPIE NanoScience + Engineering Conference
  • Place of Presentation: San Diego Convention Center （San Diego, USA)
  • Year and Date: 2015-08-09
  • Int'l Joint Research
• [Presentation] Formation of Cytochrome cb562 Oligomers by Domain Swapping (2015)
  • Author(s): Takaaki Miyamoto, Mai Kuribayashi, Satoshi Nagao, Yasuhito Shomura, Yoshiki Higuchi, Shun Hirota
  • Organizer: 29th Annual Symposium of The Protein Society
  • Place of Presentation: Palau de Congressos de Barcelona (Barcelona, Spain)
  • Year and Date: 2015-07-24
  • Int'l Joint Research
• [Presentation] Construction of Dimeric Heme Proteins by Domain Swapping (2015)
  • Author(s): Shun Hirota
  • Organizer: 17th International Conference on Biological Inorganic Chemistry (ICBIC17)
  • Place of Presentation: China National Convention Center (Beijing, China)
  • Year and Date: 2015-07-20
  • Int'l Joint Research / Invited
• [Presentation] Cytochrome c and Myoglobin Oligomers Formed by Domain Swapping (2015)
  • Author(s): Shun Hirota
  • Organizer: International Symposium on Metal Complexes (ISMEC2015)
  • Place of Presentation: Hotel Plaza (Wroclaw, Poland)
  • Year and Date: 2015-06-24
  • Int'l Joint Research / Invited
• [Presentation] Change in Structure and Ligand Binding Properties of Cytochrome c555 from Hyperthermophile by Domain Swapping (2015)
  • Author(s): 山中優、長尾聡、小森博文、樋口芳樹、廣田俊
  • Organizer: 第15回日本蛋白質科学会年会
  • Place of Presentation: あわぎんホール（徳島県徳島市）
  • Year and Date: 2015-06-24
• [Presentation] NMRによるシトクロムcとカルジオリピンの相互作用解析 (2015)
  • Author(s): 小林紀、長尾聡、廣田俊
  • Organizer: 第15回日本蛋白質科学会年会
  • Place of Presentation: あわぎんホール（徳島県徳島市）
  • Year and Date: 2015-06-24
• [Presentation] Domain Swapping of c-Type Cytochromes and Myoglobin (2015)
  • Author(s): Shun Hirota, Satoshi Nagao, Masaru Yamanaka, Hirofumi Komori, Yasuhito Shomura, Yoshiki Higuchi
  • Organizer: "Metals in Biology" in Wako
  • Place of Presentation: Riken Wako Campus (Wako, Saitama)
  • Year and Date: 2015-06-16
  • Int'l Joint Research
• [Presentation] ドメインスワップしたシトクロムcb562の構造と性質 (2015)
  • Author(s): 宮本昂明、栗林麻衣、長尾聡、庄村康人、樋口芳樹、廣田俊
  • Organizer: 第25回金属の関与する生体関連反応シンポジウム
  • Place of Presentation: 長崎大学（長崎県長崎市）
  • Year and Date: 2015-05-30
• [Presentation] ドメインスワッピングを利用したシトクロムcおよびミオグロビンの多量体形成 (2015)
  • Author(s): 長尾聡、山中優、Ying-Wu Lin、Megha Subhash Deshpande、小森博文、庄村康人、樋口芳樹、廣田俊
  • Organizer: 第25回金属の関与する生体関連反応シンポジウム
  • Place of Presentation: 長崎大学（長崎県長崎市）
  • Year and Date: 2015-05-30
• [Presentation] Crystal structure and function of a domain-swapped cytochrome cb562 dimer (2015)
  • Author(s): Takaaki Miyamoto, Mai Kuribayashi, Satoshi Nagao, Yasuhito Shomura, Yoshiki Higuchi, Shun Hirota
  • Organizer: 95th Spring Conference of the Chemical Society of Japan
  • Place of Presentation: Nihon University (Funabashi)
  • Year and Date: 2015-03-29
• [Presentation] Construction of high-order domain-swapped oligomers of Hydrogenobacter thermophilus cytochrome c552 by hinge loop mutation (2015)
  • Author(s): Chunguang Ren, Masaru Yamanaka, Satoshi Nagao, Shun Hirota
  • Organizer: 95th Spring Conference of the Chemical Society of Japan
  • Place of Presentation: Nihon University (Funabashi)
  • Year and Date: 2015-03-29
• [Presentation] Design and properties of domain-swapped dimeric myoglobin (2015)
  • Author(s): Satoshi Nagao, Ying-Wu Lin, Haruto Ishikawa, Takuya Yamada, Mohan Zhang, Yasuhito Shomura, Yasuhisa Mizutani, Yoshiki Higuchi, Shun Hirota
  • Organizer: 95th Spring Conference of the Chemical Society of Japan
  • Place of Presentation: Nihon University (Funabashi)
  • Year and Date: 2015-03-27
• [Presentation] Formation of domain-swapped oligomer of Hydrogenobacter thermophiles cytochrome c552 in an expression system (2015)
  • Author(s): Yugo Hayashi, Satoshi Nagao, Masaru Yamanaka, Hirofumi Komori, Yoshiki Higuchi, Shun Hirota
  • Organizer: 95th Spring Conference of the Chemical Society of Japan
  • Place of Presentation: Nihon University (Funabashi)
  • Year and Date: 2015-03-26
• [Presentation] 緑膿菌由来シトクロムc551と好熱性水素細菌由来シトクロムc552のキメラタンパク質を用いたヘテロダイマーの構築 (2015)
  • Author(s): 中西司、山中優、廣田俊
  • Organizer: 日本化学会第95春季年会
  • Place of Presentation: 日本大学（船橋）
  • Year and Date: 2015-03-26
• [Presentation] ヘムタンパク質の多量化：タンパク質変性と機能化（2） (2015)
  • Author(s): 廣田俊
  • Organizer: 分子研研究会「生物無機化学の最先端と今後の展望：金属と生体分子の相互作用機序解明とモデル化および応用への展開」
  • Place of Presentation: 分子科学研究所（岡崎市）
  • Year and Date: 2015-01-06
  • Invited
• [Presentation] Design and construction of protein supramolecules for anticancer agent and DDS (2014)
  • Author(s): Shun Hirota
  • Organizer: Medan International Conference on Advanced Pharmaceutical Sciences (MICAPS 2014)
  • Place of Presentation: Medan (Indonesia)
  • Year and Date: 2014-11-03
  • Invited
• [Presentation] シトクロム cb562の多量化と二量体構造 (2014)
  • Author(s): 宮本昂明、栗林麻衣、長尾聡、庄村康人、樋口芳樹、廣田俊
  • Organizer: 第8回バイオ関連化学シンポジウム
  • Place of Presentation: 岡山大学（岡山市）
  • Year and Date: 2014-09-12
• [Presentation] 生理的条件下でのシトクロムc多量体形成 (2014)
  • Author(s): 林有吾、中山璃子、長尾聡、山中優、小森博文、樋口芳樹、廣田俊
  • Organizer: 第8回バイオ関連化学シンポジウム
  • Place of Presentation: 岡山大学（岡山市）
  • Year and Date: 2014-09-12
• [Presentation] ドメインスワッピングによるヘムタンパク質の会合 (2014)
  • Author(s): 廣田俊
  • Organizer: 第24回金属の関与する生体関連反応シンポジウム
  • Place of Presentation: 京都薬科大学（京都市）
  • Year and Date: 2014-06-14
  • Invited
• [Presentation] ドメインスワッピングを利用したヘムタンパク質の超分子化 (2014)
  • Author(s): 廣田俊
  • Organizer: 第41回生体分子科学討論会
  • Place of Presentation: 九州大学（福岡市）
  • Year and Date: 2014-06-07
  • Invited