Development of novel platform for enzyme utilization in the body based on polymeric nanocapsules
| Project/Area Number |
26288082
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
Kishimura Akihiro 九州大学, 工学(系)研究科(研究院), 准教授 (70422326)
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| Research Collaborator |
TANG Hengmin 九州大学, 大学院システム生命科学府
NAOYAMA Kenshiro 九州大学, 大学院システム生命科学府
YAMASAKI Hokuto 九州大学, 大学院システム生命科学府
TERAUCHI Mikio 九州大学, 大学院システム生命科学府
OGAWA Atsushi
LIU Yiwei 九州大学, 大学院システム生命科学府
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Keywords | 酵素 / ナノリアクター / ベシクル / タンパク質デリバリー / ナノ医薬品 / Biodetoxification / ナノメディシン |
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| Outline of Final Research Achievements |
In this research, a novel platform for enzyme application, which allows for expansion of its scope and durability of enzyme activity in in vivo conditions, was pioneered, particularly using polymeric hollow capsule PICsomes with a size of ~100 nm. In this project, the following three topics were mainly investigated: (1) Enzyme-based biodetoxification nanosystem, (2) Nano-delivery system for enzyme replacement therapy, (3) Nano-delivery system of light source in in vivo conditions. In the topic of (1), PICsome containing histamine degradation enzyme was developed, and successful performance was confirmed in in vivo experiments using model mice. In the topic of (2), milder methods for enzyme encapsulation was developed, and effective factor for cellular internalization of the PICsome system was revealed. In the topic (3), bioluminescent enzyme, Nano-Luc, was successfully encapsulated into PICsomes, and its activity was confirmed for future development of light-driven nanosystem.
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Report
(4 results)
Research Products
(78 results)
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[Journal Article] Calcium phosphate-based organic-inorganic hybrid nanocarriers with pH-responsive on/off switch for photodynamic therapy2016
Author(s)
T. Nomoto, S. Fukushima, M. Kumagai, K. Miyazaki, A. Inoue, P. Mi, Y. Maeda, K. Toh, Y. Matsumoto, Y. Morimoto, A. Kishimura, N. Nishiyama, K. Kataoka
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Journal Title
Biomater. Sci.
Volume: 印刷中
Issue: 5
Pages: 826-838
DOI
Related Report
Peer Reviewed
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[Journal Article] Systemically Injectable Enzyme-Loaded Polyion Complex Vesicles as In Vivo Nanoreactors Functioning in Tumors.2016
Author(s)
Anraku Y, Kishimura A, Kamiya M, Tanaka S, Nomoto T, Toh K, Matsumoto Y, Fukushima S, Sueyoshi D, Kano MR, Urano Y, Nishiyama N, Kataoka K.
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Journal Title
Angew Chem Int Ed Engl.
Volume: 55
Issue: 2
Pages: 560-565
DOI
Related Report
Peer Reviewed
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[Journal Article] Density-Tunable Conjugation of Cyclic Rgd Ligands with Polyion Complex Vesicles for the Neovascular Imaging of Orthotopic Glioblastomas2015
Author(s)
Kawamura W, Miura Y, Kokuryo D, Toh K, Yamada N, Nomoto T, Matsumoto Y, Sueyoshi D, Liu X, Aoki I, Kano MR, Nishiyama N, Saga T, Kishimura A, Kataoka K
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Journal Title
Science and Technology of Advanced Materials
Volume: 16
Issue: 3
Pages: 035004-035004
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] 酵素機能を活かす材料開発2016
Author(s)
岸村 顕広
Organizer
ミニワークショップ「生体関連機能化学の最先端」
Place of Presentation
横浜市（東京工業大学すずかけ台キャンパス）
Year and Date
2016-06-25
Related Report
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[Presentation] Development of enzyme-loaded polyion complex vesicles for in vivo nanoreactor.2015
Author(s)
Yasutaka Anraku, Mako Kamiya, Sayaka Tanaka, Takahiro Nomoto, Kazuko Toh, Yu Matsumoto, Shigeto Fukushima, Daiki Sueyoshi, Akihiro Kishimura, Mitsunobu Kano, Yasuteru Urano, Nobuhiro Nishiyama, Kazunori Kataoka
Organizer
THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES 2015 (Pacifichem2015)
Place of Presentation
Hawaii Convention Center, Honolulu, Hawaii, USA
Year and Date
2015-12-16
Related Report
Int'l Joint Research
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