| Budget Amount *help |
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Outline of Final Research Achievements |
In this study, genetic structure and substrate specificity of biodegradable plastic-degrading enzymes derived from microorganisms and their industrial application were investigated. First, the whole genome analysis of Roseateles depolymerans TB-87 was performed to identify its degradation enzyme genes and analyzed the homology with the enzyme derived from TB-71. Furthermore, functional analysis of chaperone-like protein and related enzymes of other plastic-degrading bacteria were carried out to clarify the relationship between amino acid sequence and substrate specificity. Furthermore, culture conditions and medium cost were examined for high expression of these microbial enzymes.
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