Silk nanofibers of aquoue insects and new biomaterials
| Project/Area Number |
26288101
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Polymer/Textile materials
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
平林 公男 信州大学, 学術研究院繊維学系, 教授 (20222250)
大川 浩作 信州大学, 学術研究院繊維学系, 教授 (60291390)
野村 隆臣 信州大学, 学術研究院繊維学系, 准教授 (90362110)
新井 亮一 信州大学, 学術研究院繊維学系, 准教授 (50344023)
阿部 康次 信州大学, 学術研究院繊維学系, 教授 (00126658)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | E2Pタンパク質 / 蚕シルク / 細胞接着 / ナノファイバー / 細胞足場材 / 水生昆虫 / シルクナノ繊維 / シルク / ナノファイバ / 細胞培養 / 足場材 / 水生昆虫シルク / 足場材料 / 骨芽細胞 / シルクタンパク質 / フィブロインコンポジット / 3次元織布 / 幹細胞 / 再生医療 / シルク繊維 / バイオメディカルマテリアル / ナノファバー |
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| Outline of Final Research Achievements |
The goal of our research program is to produce silk protein materials from S. marmorata, aqueous insect, which can be used as a scaffold for tissue engineering. We prepared then the silk protein film, made of E2P, which contains Smsp-1 core component from aqueous silk fiber. E2P transparent film prepared thus was insolubilized by heat and/or ethanol treatment. E2P film enhanced MC3T3-E1 osteoblast-like cells. These findings suggest E2P film confirmed to be an efficient bioactive protein to mediate cell-biomaterial interaction.
Additionally, we are succeeded in preparing the SF composite nanofibers made of silk fibroin and compatible high polymers. Silk composite nanofibers showed that the incorporation of SF into compatibles high polymers enhanced Murine fibroblasts [NH3T3] cell or human keratinocytes cell adhesion and proliferation. These findings suggest silk composites materials confirmed to be an efficient bioactive protein to mediate cell-biomaterial interaction.
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Report
(5 results)
Research Products
(128 results)
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[Journal Article] Self-assembling supramolecular nanostructures constructed from de novo extender protein nanobuilding blocks,2018
Author(s)
Kobayashi, N., Inano, K., Sasahara, K., Sato, T., Miyazawa, K., Fukuma, T., Hecht, M.H., Song, C., Murata, K., Ara.R.
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Journal Title
ACS Synth. Biol.
Volume: 7
Pages: 1381-1394
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Peer Reviewed
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[Journal Article] Flight density of the aquatic insect fauna over the water surface in the middle reaches of the Shinano River, Japan, mainly among caddisflies (Trichoptera)2016
Author(s)
Hirabayashi, K.*, Ikutama, E., Ohkawa, K., Arai, R., Nomura, T., Tsukada, M., and Abe, K
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Journal Title
Zoosymposia
Volume: 10
Pages: 203-213
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Characterization of silk gland ribosomes from a bivoltine caddisfly, Stenopsyche marmorata: translational suppression of a silk protein in cold conditions2016
Author(s)
Nomura, Takaomi; Ito, Miho; Kanamori, Mai; Shigeno, Yuta; Uchiumi, Toshio; Arai, Ryoichi; Tsukada, Masuhiro; Hirabayashi, Kimio; Ohkawa, Kousaku
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Journal Title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume: 469
Pages: 210-215
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Molecular cloning, gene expression analysis, and recombinant protein expression of novel silk proteins from larvae of a retreat-maker caddisfly, Stenopsyche marmorata2015
Author(s)
Bai, Xue; Sakaguchi, Mayo; Yamaguchi, Yuko; Ishihara, Shiori; Tsukada, Masuhiro; Hirabayashi, Kimio; Ohkawa, Kousaku; Nomura, Takaomi; Arai, Ryoichi
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Journal Title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume: 464
Pages: 814-819
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] Biochemical Characterization of Stenopyche marmorata Silk Gland Proteins, Smsp-1 and Smsp-42014
Author(s)
Ohkawa, K., Nomura, T., Arai, R., Hirabayashi, K., Tsukada, M., and Abe, K.
Organizer
International Symposium on Fiber Science and Technology 2014 (ISF2014)
Place of Presentation
Tokyo Fushion Town（Tokyo）
Year and Date
2014-09-28 – 2014-10-01
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[Presentation] Direct solubilization of phosphorylated proteins from larval adhesive net of caddisfly, Stenopsyche marmorata2014
Author(s)
Ohkawa, K., Nomura, T., Arai, R., Hirabayashi, K., Tsukada, M., and Abe, K.
Organizer
2nd International Conference on Biological and Biomimetic Adhesives (COST Action TD0906)
Place of Presentation
Sultanahmet-Istanbul, Turkey
Year and Date
2014-05-06 – 2014-05-09
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