Development of biomimetic super-low friction articulating tissue and evaluation of its mechanical and tribological function

• Project/Area Number: 26289029
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Design engineering/Machine functional elements/Tribology
• Research Institution: Kyushu University
• Principal Investigator: Sawae Yoshinori 九州大学, 工学(系)研究科(研究院), 教授 (10284530)
• Co-Investigator(Kenkyū-buntansha): 山口 哲生 九州大学, 工学(系)研究科(研究院), 准教授 (20466783) ; 森田 健敬 九州大学, 工学(系)研究科(研究院), 助教 (70175636)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000); Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2015: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000); Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: 低摩擦 / 細胞・組織 / 関節軟骨 / トライボロジー / 再生医療

Outline of Final Research Achievements

The novel culture method for cartilage regeneration was explored to obtain highly functional articulating tissue with the cartilage-like super-low friction characteristic by reproducing the loading condition within the cartilage tissue under walking and exposing chondrocytes being cultured in the scaffold to it. A glass roller was indented into the flat scaffold surface and make a rolling-sliding motion to exert inhomogeneous and transient stress and strain field in the scaffold, which thought to be essential to simulate the mechanical condition inside the cartilage tissue under walking. The experimental system for evaluating the frictional characteristic of elaborated articular tissue was also developed. This system was enabled to measure the deformation of each chondrocyte in the cultured tissue during sliding test since the relationship between the cellular deformation caused by friction at the tissue surface and subsequent functional development of cultured tissue quantitatively.

Research Products

• [Journal Article] The Influence of Proteins and Speed on Friction and Adsorption of Metal/UHMWPE Contact Pair (2017)
  • Author(s): Necas,D, Sawae,Y, Fujisawa,T., Nakashima, K., Morita,T., Yamaguchi,T., Vrbka, M., Krupka, I., Hartl, M.
  • Journal Title: Biotribology Volume: 印刷中
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Importance of Adaptive Multimode Lubrication Mechanism in Natural Synovial Joints (2017)
  • Author(s): T. Murakami, S. Yarimitsu, N. Sakai, K. Nakashima, T. Yamaguchi, Y. Sawae
  • Journal Title: Tribology International Volume: 印刷中 Pages: 306-315
  • DOI: 10.1016/j.triboint.2016.12.052
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Improved wear resistance of functional diamond like carbon coated Ti–6Al–4V alloys in an edge loading conditions (2016)
  • Author(s): Choudhury, D., Lackner, J.M., Major, L., Morita, T., Sawae, Y., Bin Mamat, A., Stavness, I., Roy, C.K., Krupka, I.
  • Journal Title: J Mech Behav Biomed Mater Volume: 59 Pages: 586-595
  • DOI: 10.1016/j.jmbbm.2016.04.004
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Influence of Elastic Recovery of Machining Surface Asperities on the Contact Stress and Plastic Strain of Polyethylene Tibial Insert in Virgin Knee Prosthesis (2016)
  • Author(s): Cho, C., Murakami, T., Sawae, Y.
  • Journal Title: Tribology Online Volume: 11 Issue: 2 Pages: 320-325
  • DOI: 10.2474/trol.11.320
  • NAID: 130005149914
  • ISSN: 1881-218X, 1881-2198
  • Open Access
• [Journal Article] A novel functional layered diamond like carbon coating for orthopedics applications (2016)
  • Author(s): Choudhury, D., Morita, T., Sawae, Y., Lackner, J.M., Towler, M., Krupka, I.
  • Journal Title: Diamond and Related Materials Volume: 61 Pages: 56-59
  • DOI: 10.1016/j.diamond.2015.11.011
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A functional effect of the superficial mechanical properties of articular cartilage as a load bearing system in a sliding condition (2016)
  • Author(s): N. Sakai, C. Hashimoto, S. Yarimitsu, Y. Sawae, M. Komori, T. Murakami
  • Journal Title: Biosurface and Biotribology Volume: 2 Issue: 1 Pages: 26-39
  • DOI: 10.1016/j.bsbt.2016.02.004
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effect of radiationdose on depth-dependent oxidation and wear of shelf-aged gamma-irradiated ultra-high molecular weight polyethylene (UHMWPE) (2015)
  • Author(s): Zhang, L., Sawae, Y., Yamaguchi, T., Murakami, T., Yang, H.
  • Journal Title: Tribology International Volume: 89 Pages: 78-85
  • DOI: 10.1016/j.triboint.2014.12.011
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Investigation on Oxidation of Shelf-Aged Crosslinked Ultra-High Molecular Weight Polyethylene (UHMWPE) and Its Effects on Wear Characteristics (2015)
  • Author(s): L. Zhang, Y. Sawae, T. Yamaguchi, T. Murakami and H. Yang
  • Journal Title: Tribology Online Volume: 10 Issue: 1 Pages: 1-10
  • DOI: 10.2474/trol.10.1
  • NAID: 130004948471
  • ISSN: 1881-218X, 1881-2198
  • Peer Reviewed / Open Access
• [Journal Article] Effect ofradiationdoseondepth-dependentoxidationandwear of shelf-agedgamma-irradiatedultra-highmolecularweight polyethylene(UHMWPE) (2015)
  • Author(s): Lei Zhang, YoshinoriSawae, TetsuoYamaguchi, TeruoMurakami, HongYang
  • Journal Title: Tribology International Volume: *
  • Peer Reviewed
• [Journal Article] Superior Lubricity in Articular Cartilage and Artificial Hydrogel Cartilage (2014)
  • Author(s): T. Murakami, S. Yarimitsu, K. Nakashima, T. Yamaguchi, Y. Sawae, N. Sakai and A. Suzuki
  • Journal Title: Proc IMechE Part J : J Engineering Tribology Volume: 228 Issue: 10 Pages: 1099-1111
  • DOI: 10.1177/1350650114530273
  • Peer Reviewed
• [Journal Article] 人工関節における高分子材料のトライボロジー (2014)
  • Author(s): 澤江義則
  • Journal Title: 高分子 Volume: 63 Pages: 285-287
• [Presentation] 摺動負荷培養下での再生軟骨組織モデル内部応力分布変化による ECM 産生への影響 (2017)
  • Author(s): 福田圭祐，小俣誠二，澤江義則
  • Organizer: 第37回バイオトライボロジシンポジウム
  • Place of Presentation: 東京
• [Presentation] 培養軟骨組織のトライボロジー評価 (2017)
  • Author(s): 執行佳史，福田圭祐，澤江義則
  • Organizer: 第37回バイオトライボロジシンポジウム
  • Place of Presentation: 東京
• [Presentation] Frictional Characteristics of Hip Joint Prosthesis with Hydrogel Acetabular Liner (2016)
  • Author(s): Ryosuke Baba, Yoshinori Sawae, Takehiro Morita, Tetsuo Yamaguchi, Teruo Murakami
  • Organizer: International Conference on Engineering Tribology and Applied Technology 2016
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2016-11-04
  • Int'l Joint Research / Invited
• [Presentation] 歩行条件下におけるハイドロゲル人工軟骨を有する人工股関節の摩擦評価 (2016)
  • Author(s): 馬場亮佑，澤江義則，森田健敬，山口哲生
  • Organizer: サステイナブルトライボロジー会議2016奄美大島
  • Place of Presentation: 鹿児島県奄美市
  • Year and Date: 2016-10-31
• [Presentation] 歩行条件下におけるハイドロゲル人工軟骨を有する人工股関節の摩擦評価 (2016)
  • Author(s): 馬場亮佑，澤江義則，森田建敬，山口哲生，小俣誠二，村上 輝夫
  • Organizer: 日本機械学会2016年度年次大会
  • Place of Presentation: 福岡市
  • Year and Date: 2016-09-11
• [Presentation] Influence of protein adsorption on friction of metal/UHMWPE sliding pair (2016)
  • Author(s): D. Necas, Y. Sawae, T. Fujisawa, K. Nakashima, T. Morita, T. Yamaguchi, M. Vrbka, I. Krupka, M. Hartl
  • Organizer: 3rd International Conference on BioTribology 2016
  • Place of Presentation: London, UK
  • Year and Date: 2016-09-11
  • Int'l Joint Research
• [Presentation] FRICTIONAL CHARACTERISTICS OF HIGHLY HYDRATED HYDROGEL ARTIFICIAL CARTILAGE (2016)
  • Author(s): Y. Sawae, R. Baba, Y. Hong, T. Yamaguchi, T. Morita
  • Organizer: 4th Malaysia-Japan Tribology Symposium 2016
  • Place of Presentation: Kuala Lumpur, Malaysia
  • Year and Date: 2016-08-25
  • Int'l Joint Research
• [Presentation] Wear Mechanism of Polyethylene in Joint Prosthesis (2016)
  • Author(s): Yoshinori Sawae, Lei Zhang
  • Organizer: The 7th Advanced Forum on Tribology 2016, Nara
  • Place of Presentation: 奈良県橿原市
  • Year and Date: 2016-04-09
  • Int'l Joint Research / Invited
• [Presentation] 人工関節用超高分子量ポリエチレン の摩擦に及ぼす血清タンパク質の影 響 (2016)
  • Author(s): 藤澤寿洋，澤江義則， David Necas，森田健敬，中嶋和弘， 山口哲生
  • Organizer: 日本機械学会 九州支部第69期総会・講演会
  • Place of Presentation: 熊本
  • Year and Date: 2016-03-15
• [Presentation] Influence of the traction loads on ECM production and distribution in regenerated cartilage tissue (2016)
  • Author(s): K. Fukuda, S. Omata, Y. Sawae
  • Organizer: 3rd International Conference on BioTribology 2016
  • Place of Presentation: London, UK
  • Int'l Joint Research
• [Presentation] ローラーによるしゅう動負荷が再生軟骨組織モデルのECM産生に及ぼす影響 (2016)
  • Author(s): 福田圭祐，澤江義則
  • Organizer: 第38回バイオトライボロジ研究会
  • Place of Presentation: 静岡県裾野市
• [Presentation] 培養軟骨組織モデルの表面性状評価 (2016)
  • Author(s): 執行佳史，澤江義則
  • Organizer: 第38回バイオトライボロジ研究会
  • Place of Presentation: 静岡県裾野市
• [Presentation] 培養再生軟骨組織への定量的動的せん断ひずみ負荷と内部細胞の変形定量に関する研究 (2016)
  • Author(s): 有浦秀昭，澤江義則
  • Organizer: 第38回バイオトライボロジ研究会
  • Place of Presentation: 静岡県裾野市
• [Presentation] Effects of oxidative degradation on the wear and wear particles of cross-linked UHMWPE (2015)
  • Author(s): Lei Zhang, Yoshinori Sawae, Tetsuo Yamaguchi, Teruo Murakami
  • Organizer: Malaysian International Tribology Conference 2015
  • Place of Presentation: Penang, Malaysia
  • Year and Date: 2015-11-16
  • Int'l Joint Research
• [Presentation] 表面に対する摺動負荷が再生軟骨組織形成に及ぼす影響 (2015)
  • Author(s): 福田圭祐，小俣誠 二，澤江義則
  • Organizer: 第 26 回バイオフロンティア講演会
  • Place of Presentation: 福岡
  • Year and Date: 2015-10-02
• [Presentation] Behavior of Protein Film Adsorbed on Joint Prosthesis Material under Rubbing (2015)
  • Author(s): Kazuhiro Nakashima, Yoshinori Sawae, Susumu Kudo, Teruo Murakami
  • Organizer: The 8th International Biotribology Forum
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2015-09-21
  • Int'l Joint Research
• [Presentation] ECM Production in Chondrocyte-Agarose Construct Cultured under Traction Loading (2015)
  • Author(s): Keisuke Fukuda, Seiji Omata, Yoshinori Sawae
  • Organizer: The 8th Asian-Pacific Conference on Biomechanics
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2015-09-16
  • Int'l Joint Research
• [Presentation] Effect of High Gamma-Ray Irradiation Dose on Oxidation and Wear of Cross-Linked Polyethylene (2015)
  • Author(s): Lei Zhang, Yoshinori Sawae, Tetsuo Yamaguchi, Teruo Murakami
  • Organizer: International Tribology Conference, Tokyo 2015
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2015-09-16
  • Int'l Joint Research
• [Presentation] ECM produc6on and distribu6on in regenerated car6lage 6ssue cultured under trac6on loading (2015)
  • Author(s): Keisuke Fukuda, Yoshinori Sawae
  • Organizer: IUPESM World Congress 2015
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2015-06-07
  • Int'l Joint Research
• [Presentation] 人工関節材料の摩擦摩耗特性へ影響 する蛋白質吸着膜の観察 (2015)
  • Author(s): 中嶋和弘，澤江義則，工藤奨，村上 輝夫
  • Organizer: トライボロジー会議 2015春 姫路
  • Place of Presentation: 姫路市
  • Year and Date: 2015-05-27
• [Presentation] Biotribology Research in Japan (2014)
  • Author(s): Yoshinori Sawae
  • Organizer: Czech-Japan Tribology Workshop
  • Place of Presentation: Mikulov, Czech Republic
  • Year and Date: 2014-11-24 – 2014-11-27
  • Invited
• [Presentation] Development of anisotropic cartilage tissue in chondrocyte-agarose construct cultured under traction loading (2014)
  • Author(s): Keisuke Fukuda, Seiji Omata, Yoshinori Sawae
  • Organizer: 2014 International Conference on Engineering Tribology Technology
  • Place of Presentation: Taiwan
  • Year and Date: 2014-11-21 – 2014-11-23
• [Presentation] Investigation of wear and wear particles from shelf-aged crosslinked UHMWPE under different contact pressures (2014)
  • Author(s): Lei Zhang, Yoshinori Sawae, Teruo Murakami, Ken Yuki
  • Organizer: 3rd Malaysia-Japan Tribology Symposium 2014
  • Place of Presentation: Kuala lumpr, Malaysia
  • Year and Date: 2014-11-12 – 2014-11-14
• [Presentation] 架橋ポリエチレンの酸化劣化と摩耗への影響 (2014)
  • Author(s): 張磊，澤江義則，山口哲生，森田健敬，村上輝夫
  • Organizer: トライボロジー会議 2014秋 盛岡
  • Place of Presentation: 岩手県盛岡市
  • Year and Date: 2014-11-05 – 2014-11-07
• [Presentation] 再生軟骨組織モデル表面に対する摺動負荷によるECMの分布への影響 (2014)
  • Author(s): 福田圭祐，小俣誠二，澤江義則
  • Organizer: 第25回バイオフロンティア講演会
  • Place of Presentation: 鳥取県鳥取市
  • Year and Date: 2014-10-03 – 2014-10-04
• [Presentation] Characterisation of regenerated cartilage tissue model cultured with traction loading on its surface (2014)
  • Author(s): Keisuke Fukuda, Seiji Omata, Yoshinori Sawae
  • Organizer: The 4th Japan-Switzerland Workshop on Biomechanics
  • Place of Presentation: 三重県志摩市
  • Year and Date: 2014-09-01 – 2014-09-04
• [Presentation] 人工関節材料と関節液成分のバイオトライボロジ (2014)
  • Author(s): 澤江義則
  • Organizer: 第36回バイオトライボロジ研究会
  • Place of Presentation: 静岡県裾野市
  • Year and Date: 2014-08-09
  • Invited
• [Presentation] 人工関節材料のトライボロジー特性に対する生体内環境の影響 (2014)
  • Author(s): 澤江義則
  • Organizer: 第53 回日本生体医工学会大会
  • Place of Presentation: 宮城県仙台市
  • Year and Date: 2014-06-24 – 2014-06-26
  • Invited
• [Presentation] Friction characterization of ceramic-on-hydrogel hip joint in pendulum test (2014)
  • Author(s): Y. Sawae, M. Vrbka, F. Urban, D. Necas, S. Yarimitsu, K. Nakashima, T. Murakami, M. Hartl
  • Organizer: 2nd International Conference on BioTribology
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2014-05-12 – 2014-05-14
• [Presentation] Effect of radiation dose on depth-dependent oxidation and wear of gamma-irradiated ultra-high molecular weight polyethylene (UHMWPE) (2014)
  • Author(s): L. Zhang, Y. Sawae, T. Yamaguchi, T. Murakami, H. Yang
  • Organizer: 2nd International Conference on BioTribology
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2014-05-12 – 2014-05-14
• [Presentation] Protein adsorbed film formation and frictional characteristics of CoCrMo-on-UHMWPE sliding pair in reciprocating sliding test (2014)
  • Author(s): D. Necas, Y. Sawae, S. Yarimitsu, K. Nakashima, M. Vrbka, M. Hartl, T. Murakami
  • Organizer: 2nd International Conference on BioTribology
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2014-05-12 – 2014-05-14