| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2018: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2017: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2014: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
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| Outline of Final Research Achievements |
We developed a microfluidic system that offered a highly reproducible single-cell isolation and electroporation (97%, n>120). We showed that the hydrodynamic trap effectively captured a single cell and focused the electric field for electroporation to reduce the applied voltage by ten-fold in comparison to a uniform electric field. We also developed an equation that estimated the magnitude of the electric field exerted on the nuclear membrane as a function of the distance between the hydrodynamic trap and the nuclear membrane. Furthermore, we applied our microfluidic system for integrated nuclear and cytoplasmic RNA-sequencing of single cells and coined the method SINC-seq.
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