| Project/Area Number |
26289038
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Fluid engineering
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| Research Institution | Chuo University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
OKANO Taiji 中央大学, 理工学部, 助教 (60622082)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
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| Keywords | バイオリアクター / リポソーム / 膜融合 / 膜分裂 / ゲノム増幅 / PCR / マイクロリアクター / ジャイアントリポソーム / 人工細胞膜 / 溶液操作 / マイクロ流体デバイス / 融合 / 分裂 / フェムトリットル / 反応場 |
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| Outline of Final Research Achievements |
We aimed to establish an ultra-small biochemical assay platform using giant liposomes as a femtoliter-volume reactor, which enables addition, mixing, and aliquoting. As a means of GUV fusion, we studied PEG-mediated fusion method in addition to the well-established GUV electrofusion method, and found that PEG-fusion is more suitable for fusing GUV and the live cell membrane. For aliquoting, we thoroughly studied the effect of depletion-volume effect and addition of mono-acyl lipids. Finally we demonstrated the three distinct biochemical reactions in GUVs; (1) an enzyme reaction, (2) the whole-genome amplification, and (3) the reverse transcription and polymerase chain reaction (RT-PCR) to show that the sequential handling of these reagents is possible.
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