| Budget Amount *help |
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Outline of Final Research Achievements |
DEPIM (dielectrophoretic impedance measurement) revealed that the microelectrode conductance increased with elapsed time as a result of positive DEP trap of DNA-labeled microbeads. On the other hand, intact microbeads were not trapped on the microelectrode decreasing the conductance. This result implies that the presented DNA detection method can basically works as intended.
The electrical DNA detection method was further applied to bacterial detection. Bacterial DNA was extracted from E. coli strain K12 bacteria and amplified by polymerase chain reaction (PCR). The presented method realized quantitative detection of specific bacteria at concentrations above 100000 and 24000 CFU/ml for bacterial solutions with and without other bacterial presence, respectively. It was also showed the method detected 100000 copies of pig meat DNA in the mixture of 10<sup>8</sup> copies of cow meat DNA. As the results, 5 mg of pork in 50 mg of meat mixed with beef was detected.
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