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Development of methods for microbial source tracking in drinking water sources using host-specific viral markers

Research Project

Project/Area Number 26289182
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Civil and environmental engineering
Research InstitutionUniversity of Yamanashi

Principal Investigator

HARAMOTO Eiji  山梨大学, 総合研究部, 准教授 (00401141)

Co-Investigator(Kenkyū-buntansha) 岸田 直裕  国立保健医療科学院, 生活環境研究部, 主任研究官 (10533359)
端 昭彦  京都大学, 工学研究科, 日本学術振興会特別研究員 (70726306)
北島 正章  北海道大学, 工学研究院, 助教 (30777967)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Keywordsウイルス / 濃縮法 / 糞便汚染源解析 / 健康関連微生物 / 土木環境システム / 水循環
Outline of Final Research Achievements

This study aimed to develop methods for concentrating viruses in drinking water source and drinking water, mainly focusing a NanoCeram electropositive filter. Recovery test of F-specific RNA coliphage Qbeta and ColorSeed, a commercially available product containing Cryptosporidium and Giardia (oo)cysts, was conducted to develop a method for simultaneous concentration of viruses and protozoa in a single water sample. Furthermore, recovery of F-specific RNA coliphage MS2 and pepper mild mottle virus, a plant virus, using the NanoCeram electropositive filter was tested as well as methods using electronegative membrane filters. This study has successfully developed novel virus concentration methods that can be used for microbial source tracking using viruses.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (9 results)

All 2017 2016 2015 2014

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Acknowledgement Compliant: 2 results) Presentation (7 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] Human polyomavirus: Advantages and limitations as a human-specific viral marker in aquatic environments2016

    • Author(s)
      Andri T. Rachmadi, Jason R. Torrey, Masaaki Kitajima
    • Journal Title

      Water Research

      Volume: 105 Pages: 456-469

    • DOI

      10.1016/j.watres.2016.09.010

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] バクテロイデス遺伝子マーカーを用いた河川水中のふん便汚染源解析2016

    • Author(s)
      原本英司, 渡辺亮, 森田久男, 岸田直裕, 秋葉道宏, 坂本康
    • Journal Title

      用水と廃水

      Volume: 58 Pages: 589-595

    • NAID

      40020927287

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] F特異DNA大腸菌ファージの遺伝子型検出による水環境中の糞便汚染源解析2017

    • Author(s)
      森山一葉, 長田留衣, 山田貴大, 原本英司, 坂本康
    • Organizer
      第51回日本水環境学会年会
    • Place of Presentation
      熊本大学(熊本県熊本市)
    • Year and Date
      2017-03-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] Indicators of viral contamination of water2016

    • Author(s)
      Eiji Haramoto
    • Organizer
      5th Food and Environmental Virology Conference
    • Place of Presentation
      ホテル櫻井(群馬県吾妻郡草津町)
    • Year and Date
      2016-09-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ナノセラム陽電荷膜法によるトウガラシ微斑ウイルスの濃縮回収率の測定2016

    • Author(s)
      山田貴大,原本英司,坂本康
    • Organizer
      第50回日本水環境学会年会
    • Place of Presentation
      アスティとくしま(徳島市)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] Pepper mild mottle virus and tobacco mosaic virus as potential viral indicators of human fecal contamination in river water2015

    • Author(s)
      Eiji Haramoto, Naohiro Kishida, Hisao Morita, Mari Asami, Michihiro Akiba
    • Organizer
      18th International Symposium on Health-Related Water Microbiology
    • Place of Presentation
      リスボン大学(ポルトガル・リスボン市)
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 水中ウイルス濃縮法によるトウガラシ微斑ウイルスの回収率の測定2015

    • Author(s)
      山田貴大,古屋崇志,原本英司,坂本康
    • Organizer
      第49回日本水環境学会年会
    • Place of Presentation
      金沢大学(石川県・金沢市)
    • Year and Date
      2015-03-16 – 2015-03-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] ナノセラム陽電荷膜を用いた環境水中からのウイルス及び原虫同時濃縮法の開発2014

    • Author(s)
      古屋崇志,原本英司,坂本康
    • Organizer
      第51回環境工学研究フォーラム
    • Place of Presentation
      山梨大学(山梨県・甲府市)
    • Year and Date
      2014-12-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] 微生物遺伝子マーカーを用いた水道水源河川中の糞便汚染源解析2014

    • Author(s)
      渡辺亮,黒川恵未,原本英司,森田久男,岸田直裕,浅見真理,秋葉道宏,坂本康
    • Organizer
      第51回環境工学研究フォーラム
    • Place of Presentation
      山梨大学(山梨県・甲府市)
    • Year and Date
      2014-12-21
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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