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Establishment of the in vitro docking (IVD) method for the functional expression of human membrane proteins on magnetic particles

Research Project

Project/Area Number 26289308
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Yoshino Tomoko  東京農工大学, 工学(系)研究科(研究院), 准教授 (30409750)

Co-Investigator(Kenkyū-buntansha) 前田 義昌  東京農工大学, 工学(系)研究科(研究院), 助教 (30711155)
Co-Investigator(Renkei-kenkyūsha) TANAKA TSUYOSHI  東京農工大学, 大学院工学研究院, 教授 (20345333)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Keywords磁性粒子 / 膜タンパク質 / in vitro docking / 細胞内区画 / SS結合
Outline of Final Research Achievements

This study aimed to establish the in vitro docking (IVD) method in the magnetotactic bacterium towards the development of the functional composites of bacterial magnetic particles (BacMPs) and human-derived membrane proteins. In the IVD method, recombinant proteins (including membrane proteins) expressed in the periplasmic space of the magnetotactic bacterium were assembled onto BacMPs, which were separately synthesized in the identical bacterium, with the aid of docking protein pairs after cell disruption. Several types of docking protein pairs were examined for the IVD method. Subsequently, as a proof-of-concept, thyroid stimulating hormone receptor (TSHR) was displayed on the BacMPs through the IVD method. In addition, the minimum expression system was established in the magnetotactic bacterium for appropriate protein folding, which could be beneficial for functional expression of recombinant proteins.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (16 results)

All 2017 2016 2015 2014

All Journal Article (7 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 2 results) Presentation (9 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results)

  • [Journal Article] 磁性細菌が生合成する磁気微粒子のインターフェイス設計2016

    • Author(s)
      吉野知子
    • Journal Title

      生物工学会誌

      Volume: 94 Pages: 690-692

    • Related Report
      2016 Annual Research Report
  • [Journal Article] 効率的なセルロース分解に向けた多酵素-磁性粒子複合体の生合成2016

    • Author(s)
      吉野知子、本多亨
    • Journal Title

      月刊バイオインダストリー

      Volume: 33 Pages: 79-85

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Stoichiometrically controlled immobilization of multiple enzymes on magnetic nanoparticles by the magnetosome display system for efficient cellulose hydrolysis2015

    • Author(s)
      Toru Honda, Tsuyoshi Tanaka, Tomoko Yoshino
    • Journal Title

      Biomacromolecules

      Volume: 16 Issue: 12 Pages: 3863-3868

    • DOI

      10.1021/acs.biomac.5b01174

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Functional expression of full-length TrkA in the prokaryotic host Magnetospirillum magneticumAMB-1 by using a magnetosome-display system2015

    • Author(s)
      Toru Honda, Takayuki Yasuda, Tsuyoshi Tanaka, Koji Hagiwara, Tohru Arai, Tomoko Yoshino
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 81 Issue: 4 Pages: 1472-1476

    • DOI

      10.1128/aem.03112-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel Designs of Single-chain MHC I/peptide Complex for the Magnetosome Display System2015

    • Author(s)
      Toru Honda, Yoshiaki Maeda, Takayuki Yasuda, Tsuyoshi Tanaka, Tadashi Matsunaga, Tomoko Yoshino
    • Journal Title

      Pro. Eng. Des. Select.

      Volume: 28 Issue: 2 Pages: 53-58

    • DOI

      10.1093/protein/gzu056

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] タンパク質-磁性粒子複合体の in vivo 合成と創薬への展開2015

    • Author(s)
      吉野知子、本田亨
    • Journal Title

      BIO INDUSTRY

      Volume: 32 Pages: 65-70

    • Related Report
      2014 Annual Research Report
  • [Journal Article] 微生物を用いたタンパク質-ナノ磁性粒子複合体の生合成2014

    • Author(s)
      吉野知子、前田義昌
    • Journal Title

      オレオサイエンス

      Volume: 14 Pages: 433-438

    • NAID

      130005308184

    • Related Report
      2014 Annual Research Report
  • [Presentation] 磁性粒子上へのセルラーゼ複合体の共局在によるセルロース分解能の向上2017

    • Author(s)
      田山爽也華、本多亨、伊藤康仁、田中剛、吉野知子
    • Organizer
      日本化学会第97春季大会
    • Place of Presentation
      慶応義塾大学日吉キャンパス、神奈川
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] マグネットソームディスプレイ法による時期微粒子インターフェイスの分子デザイン2017

    • Author(s)
      吉野知子
    • Organizer
      最新バイオインターフェース研究会
    • Place of Presentation
      三重大学生物資源学部、三重県
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 磁性細菌を用いたナノ磁性粒子表面の分子設計とバイオプロセス創成2016

    • Author(s)
      吉野知子
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      東北大学川内北キャンパス、宮城県
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Production of functional biomaterials using genetically engineered microbes2016

    • Author(s)
      Tomoko Yoshino
    • Organizer
      Malaysia-Japan Joint international conference
    • Place of Presentation
      Seri pacific hotel, Kuala Lumpur
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 自己抗体計測に向けた甲状腺刺激ホルモン受容体-ナノ磁性粒子複合体の開発2016

    • Author(s)
      萩原優里、本多亨、田中剛、吉野知子
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      同志社大学 京田辺キャンパス、京都
    • Year and Date
      2016-03-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] マグネトソームディスプレイによる磁性粒子上へのセルラーゼ複合体の固定化制御2015

    • Author(s)
      本多亨、田中剛、吉野知子
    • Organizer
      第67回日本生物工学会大会
    • Place of Presentation
      城山観光ホテル、鹿児島
    • Year and Date
      2015-10-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] リガンドハンティングに向けた 神経成長因子-ナノ磁性粒子の開発2015

    • Author(s)
      鹿島大揮
    • Organizer
      日本化学会第95春季年会
    • Place of Presentation
      日本大学 理工学部船橋キャンパス/薬学部
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] in vitro docking法による単鎖抗体の磁性細菌粒子上への機能発現2014

    • Author(s)
      萩原 優里
    • Organizer
      第66回日本生物工学会大会
    • Place of Presentation
      札幌コンベンションセンター, 北海道
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] タンパク質- 磁性粒子複合体の生合成と創薬探索ツールとしての活用2014

    • Author(s)
      吉野知子
    • Organizer
      新技術説明会
    • Place of Presentation
      JST東京本部別館ホール
    • Year and Date
      2014-06-17
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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