Development of the innovative process for material production using functional diatoms based on the biosilica engineering
| Project/Area Number |
26289309
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
Tanaka Tsuyoshi 東京農工大学, 工学(系)研究科(研究院), 教授 (20345333)
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| Co-Investigator(Kenkyū-buntansha) |
吉野 知子 東京農工大学, 工学(系)研究科(研究院), 准教授 (30409750)
真山 茂樹 東京学芸大学, 教育学部, 教授 (40199914)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Keywords | 有用珪藻 / バイオシリカ工学 / 自己凝集 / 有用物質生産 / 珪藻 / 珪殻 |
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| Outline of Final Research Achievements |
This study aimed to develop the innovative process for material production using functional diatoms based on the biosilica engineering, in which biosilica cell wall of diatoms were engineered via genetic manipulation. First, genetic manipulation technique for stable cell-surface display of recombinant proteins on the frustules was established in the pennate diatom, Fistulifera solaris. Subsequently, cell aggregation-inducible functional peptides were displayed on the surface of the cell walls (frustules), and the peptide-mediated cell harvesting was demonstrated. Gene knockdown technique was also established in this study in order to efficiently disrupt the cells for improved material extraction. Furthermore, structures and chemical compositions analyses for organic matrix associated with biosilica cell walls were carried out. These outcomes could pave the way to develop the novel bioprocesses for efficient material production in diatoms
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Report
(4 results)
Research Products
(52 results)
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[Journal Article] Oil accumulation by the oleaginous diatom Fistulifera solaris as revealed by the genome and transcriptome2015
Author(s)
Tsuyoshi Tanaka, Yoshiaki Maedaa, Alaguraj Veluchamy, Michihiro Tanaka, Heni Abida, Eric Marechal, Chris Bowler, Masaki Muto, Yoshihiko Sunaga, Masayoshi Tanaka, Tomoko Yoshino, Takeaki Taniguchi, Yorikane Fukuda, Michiko Nemoto, Mitsufumi Matsumoto, Pui Shan Wong, Sachiyo Aburatani, Wataru Fujibuchi
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Journal Title
The Plant Cell
Volume: 27
Issue: 1
Pages: 162-176
DOI
Related Report
Peer Reviewed
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