| Budget Amount *help |
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Outline of Final Research Achievements |
This study aimed to develop the innovative process for material production using functional diatoms based on the biosilica engineering, in which biosilica cell wall of diatoms were engineered via genetic manipulation. First, genetic manipulation technique for stable cell-surface display of recombinant proteins on the frustules was established in the pennate diatom, Fistulifera solaris. Subsequently, cell aggregation-inducible functional peptides were displayed on the surface of the cell walls (frustules), and the peptide-mediated cell harvesting was demonstrated. Gene knockdown technique was also established in this study in order to efficiently disrupt the cells for improved material extraction. Furthermore, structures and chemical compositions analyses for organic matrix associated with biosilica cell walls were carried out. These outcomes could pave the way to develop the novel bioprocesses for efficient material production in diatoms
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