Improvement of infulenza vaccine production by transgenic chicken
| Project/Area Number |
26289312
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Nagoya University |
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Principal Investigator |
Shinji Iijima 名古屋大学, 工学(系)研究科(研究院), 教授 (00168056)
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| Co-Investigator(Kenkyū-buntansha) |
小野 悦郎 九州大学, 医学(系)研究科(研究院), 教授 (00160903)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
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| Keywords | トランスジェニックニワトリ / インフルエンザ / シアル酸 / インフルエンザウイルス / ワクチン / バイオテクノロジー / ウイルス / ニワトリ / 糖鎖 / リコンビナーゼ / インフル |
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| Outline of Final Research Achievements |
In order to produce influenza vaccine effectively, we tried to establish transgenic chicken that lays eggs suitable for virus propagation. Chimeric chickens were established those expressed either phiC31 recombinase or sialic acid transferase by using retrovirus and lentivirus vectors. By crossing these chimeric chicken, we tried to establish transgenic chickens. Although we have analyzed more than 250 offspring, we could not detect transgenic chicken. The expression of IFITMs those prevent influenza virus propagation, was analyzed in chicken, and certain level of expression could be detected in chorioallantoic membrane where influenza virus propagates. We also establish in vitro and viral particle free assay system of influenza virus propagation.
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Report
(4 results)
Research Products
(4 results)
