| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
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| Outline of Final Research Achievements |
In order to produce influenza vaccine effectively, we tried to establish transgenic chicken that lays eggs suitable for virus propagation. Chimeric chickens were established those expressed either phiC31 recombinase or sialic acid transferase by using retrovirus and lentivirus vectors. By crossing these chimeric chicken, we tried to establish transgenic chickens. Although we have analyzed more than 250 offspring, we could not detect transgenic chicken. The expression of IFITMs those prevent influenza virus propagation, was analyzed in chicken, and certain level of expression could be detected in chorioallantoic membrane where influenza virus propagates. We also establish in vitro and viral particle free assay system of influenza virus propagation.
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