Development of a nucleic acid-based biosensing molecule for the detection of a desired target in a homogeneous assay format
| Project/Area Number |
26289314
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Keywords | ホモジニアスアッセイ / バイオセンサー / DNAナノピンセット構造 / 分割核酸酵素 / 蛍光共鳴エネルギー移動（FRET） / バイオイメージング / バイオセンサ / ナノバイオ / 生体機能利用 / 単一細胞解析 |
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| Outline of Final Research Achievements |
In this research, we have attempted to develop a DNA nano-tweezers structured (DNA-NT) biosensing molecule for the detection of a desired target in a homogeneous assay format that does not require washing steps.
As a results, we have continuously detected a specific mRNA in living cells with a fluorescence-based DNA-NT biosensing molecule in a homogeneous assay format, we have proved a basic concept to create a new DNA-NT biosensing molecule that detects a desired target, and we have created a split-DNazyme-based DNA-NT biosensing molecule that exhibits an enzymatic activity in response to target recognition.
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Report
(3 results)
Research Products
(17 results)
