| Budget Amount *help |
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Outline of Final Research Achievements |
In this research, we have attempted to develop a DNA nano-tweezers structured (DNA-NT) biosensing molecule for the detection of a desired target in a homogeneous assay format that does not require washing steps.
As a results, we have continuously detected a specific mRNA in living cells with a fluorescence-based DNA-NT biosensing molecule in a homogeneous assay format, we have proved a basic concept to create a new DNA-NT biosensing molecule that detects a desired target, and we have created a split-DNazyme-based DNA-NT biosensing molecule that exhibits an enzymatic activity in response to target recognition.
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