| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
|
|---|
| Outline of Final Research Achievements |
Some dipeptides have specific physiological functions such as antihypertensive and taste-improving effects. We identified that Met-Gly and Pro-Gly are the new dipeptides as salt taste enhancer. The dipetides were synthesized by L-amino acid ligase (Lal), which is a microbial enzyme that catalyzes dipeptide synthesis from unprotected L-amino acids by hydrolysis of ATP to ADP. We have developed an efficient production process of Met-Gly and Pro-Gly using Lals with structure-based site-directed mutagenesis.
Aminoacyl prolines (Xaa-Pro), which are valuable compounds because of their biological activity, have been synthesized using the adenylation domain (A domain) of nonribosomal peptide synthetase (NRPS). Utilization of ATP-dependent enzymes for industrial processes is limited by the high cost of ATP. Therefore, the ATP regeneration system from AMP or ADP has been developed using a class III polyphosphate kinase 2, which was coupled with Xaa-Pro synthesis catalyzed by A domain of NRPS.
|
