Development of amide compounds production using functional biomolecules
| Project/Area Number |
26289318
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Waseda University |
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Principal Investigator |
KINO Kuniki 早稲田大学, 理工学術院, 教授 (60318764)
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| Research Collaborator |
Kurumizaka Hitoshi
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
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| Keywords | バイオテクノロジー / 遺伝子 / 酵素 / 生体分子 / 生物・生体工学 / ペプチド / アミド化合物 / 酵素工学 |
|---|
| Outline of Final Research Achievements |
Some dipeptides have specific physiological functions such as antihypertensive and taste-improving effects. We identified that Met-Gly and Pro-Gly are the new dipeptides as salt taste enhancer. The dipetides were synthesized by L-amino acid ligase (Lal), which is a microbial enzyme that catalyzes dipeptide synthesis from unprotected L-amino acids by hydrolysis of ATP to ADP. We have developed an efficient production process of Met-Gly and Pro-Gly using Lals with structure-based site-directed mutagenesis.
Aminoacyl prolines (Xaa-Pro), which are valuable compounds because of their biological activity, have been synthesized using the adenylation domain (A domain) of nonribosomal peptide synthetase (NRPS). Utilization of ATP-dependent enzymes for industrial processes is limited by the high cost of ATP. Therefore, the ATP regeneration system from AMP or ADP has been developed using a class III polyphosphate kinase 2, which was coupled with Xaa-Pro synthesis catalyzed by A domain of NRPS.
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Report
(4 results)
Research Products
(34 results)
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[Patent(Industrial Property Rights)] 塩味増強剤2015
Inventor(s)
木野はるか, 角谷政尚, 服部宏一, 木野邦器
Industrial Property Rights Holder
木野はるか, 角谷政尚, 服部宏一, 木野邦器
Industrial Property Rights Type
特許
Industrial Property Number
2015-128323
Filing Date
2015-06-26
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