Optogenetic countrol of visual perception

• Project/Area Number: 26290011
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Benucci Andrea 国立研究開発法人理化学研究所, 脳科学総合研究センター, チームリーダー (50722352)
• Research Collaborator: Tsubota Tadashi ; Aoki Ryo
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000); Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
• Keywords: neural circuits / behavioral training / decision-making / cortex / optogenetics / two-photon imaging / Vision / animal cognition / decision making / fluorescent imaging / visual cortex / cortical plasticity / Visual Processing / Decision making / Cortex / Optogenetics / Optical Imaging / mouse / imaging / electrophysiology / behavior

Outline of Final Research Achievements

We have developed a new platform for mouse behavioral training which is high-throughput, fully automated, and features voluntary head fixation. We have used several of these setups to provide evidence mice can use perceptual concepts in sensory-based decision making tasks, a complex cognitive skill so far undemonstrated in this mammalian species. We have also developed a technology for simultaneous imaging and optogenetic perturbations of cortical networks at near single cell resolution. With this technology we are studying the in-vivo rules of functional plasticity in the cortex of adult mice.

Research Products

• [Journal Article] Local and global contributions to hemodynamic activity in mouse cortex (2016)
  • Author(s): Pisauro, M.A., Benucci, A., Carandini, M.
  • Journal Title: J Neurophysiol Volume: 115(6) Issue: 6 Pages: 2931-6
  • DOI: 10.1152/jn.00125.2016
• [Presentation] Fully automated training fixed mice. (2015)
  • Author(s): Ryo Aoki
  • Organizer: Annual Meeting of Society for Neuroscience, 2015
  • Place of Presentation: Chicago, USA
  • Year and Date: 2015-10-21
  • Int'l Joint Research
• [Presentation] Studying perceptual learning in mice with a fully-automated training system for voluntary head fixation. (2015)
  • Author(s): Andrea Benucci
  • Organizer: Sanford University School of Medicine
  • Place of Presentation: Sanford University School of Medicine, USA
  • Year and Date: 2015-09-17
  • Invited
• [Patent(Industrial Property Rights)] Fully-automated, high-throuput system for voluntary head fixation in mice (2016)
  • Inventor(s): Andrea Benucci
  • Industrial Property Rights Holder: Andrea Benucci
  • Industrial Property Rights Type: 特許
  • Filing Date: 2016-06-29