Functional analysis of commissural neurons in coordinated movements in zebrafish
Project/Area Number |
26290013
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Neurophysiology / General neuroscience
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Research Institution | Okazaki Research Facilities, National Institutes of Natural Sciences |
Principal Investigator |
Higashijima Shin-ichi 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (80270479)
|
Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
|
Keywords | 運動 / 左右協調 / 脊髄 / ゼブラフィッシュ / 交差型介在ニューロン / 左右協調運動 / 抑制 / 脊髄神経回路 / 左右強調 / リズム運動 |
Outline of Final Research Achievements |
We performed functional analysis of dI6 commissural inhibitory neurons during swimming in zebrafish. Electrophysiological analyses have revealed that (1) these neurons fired in phase with motoneurons located nearby during swimming, and that (2) these neurons made monosynaptic inhibitory connections onto motoneurons on the contralateral side. We further investigated functional roles of these neurons by genetically ablating the neurons. Larval fish in which dI6 commissural inhibitory neurons were absent frequently exhibited bilateral (not alternating) activities during swimming. These results indicate that dI6 commissural inhibitory neurons play a critical role during swimming by providing crossed inhibition to neurons on the contralateral side.
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Report
(5 results)
Research Products
(5 results)
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[Journal Article] Social conflict resolution regulated by two dorsal habenular subregions in zebrafish.2016
Author(s)
Chou, M-Y., Amo, R., Kinoshita, M., Cherng, B-W., Shimazaki, H., Agetsuma, M., Shiraki, T., Aoki, T., Yamazaki, M., Higashijima, S., and Okamoto, H.
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Journal Title
Science
Volume: 352
Issue: 6281
Pages: 87-90
DOI
Related Report
Peer Reviewed
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[Journal Article] A 3d-searchable database of transgenic zebrafish Gal4 and Cre lines for functional neuroanatomy studies.2015
Author(s)
Marquart, G.D., Tabor, K.M., Brown, M.R., Strykowski, J.L., LaFave, M.C., Varshney, G.K., Mueller, T., Burgess, S.M., Higashijima, S., and Burgess, H.A.
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Journal Title
Frontiers in Neural Circuits
Volume: 9
Pages: 78-78
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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