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Molecular mechanisms of astrocyte heterogeneity

Research Project

Project/Area Number 26290021
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

Tanaka Kohichi  東京医科歯科大学, 難治疾患研究所, 教授 (80171750)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Keywordsアストロサイト / ゲノム編集 / 脳神経疾患 / 神経科学 / 脳・神経
Outline of Final Research Achievements

Astrocytes were thought to be a homogenous population of cells. However, several studies have demonstrated that astrocytes from different regions show heterogeneity in their expression of ion channels and coupling molecules. To dissect astrocyte heterogeneity, we decode the origin of astrocytes in different brain regions. To elucidate the embryonic origins of astrocyte in a specific brain region, we applied a recombinase-based intersectional genetic strategy in mice. Astrocytes in dorsal forebrain, thalamus, hypothalamus, cerebellum/midbrain, and spinal cord are derived from Emx1-, Olig3-, Nkx2.1-, En1, Hoxb8-positive domain of the ventricular zone, respectively. In addition, intersectional approach can control the activity of astrocytes in a region-specific manner. We can demonstrate that astrocytes in different brain regions contribute to different brain functions.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (36 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (3 results) Journal Article (13 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 13 results,  Open Access: 12 results,  Acknowledgement Compliant: 3 results) Presentation (19 results) (of which Int'l Joint Research: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] The University of Edinburgh/Heriot-Watt University(英国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Johns Hopkins University(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Centre for Brain Development and Repair(インド)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Regulation of visual Wulst cell responsiveness by imprinting causes stimulus-specific activation of rostral cells.2017

    • Author(s)
      Nakamori T, Kato T, Sakagami H, Tanaka K, Ohki-Hamazaki H.
    • Journal Title

      Sci Rep.

      Volume: 7 Issue: 1 Pages: 42927-42927

    • DOI

      10.1038/srep42927

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Association of impaired neuronal migration with cognitive deficits in extremely preterm infants.2017

    • Author(s)
      Kubo K, Deguchi K, Nagai T, Ito Y, Yoshida K, Endo T, Benner S, Shan W, Kitazawa A, Aramaki M, Ishii K, Shin M, Matsunaga Y, Hayashi K, Kakeyama M, Tohyama C, Tanaka KF, Tanaka K, Takashima S, Nakayama M, Itoh M, Hirata Y, Antalffy B, Armstrong DD, Yamada K, Inoue K, Nakajima K
    • Journal Title

      JCI Insight

      Volume: 印刷中

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Posterior cerebellar Purkinje cells in an SCA5/SPARCA1 mouse model are especially vulnerable to the synergistic effect of loss of β-III spectrin and GLAST.2016

    • Author(s)
      Perkins EM, Suminaite D, Clarkson YL, Lee SK, Lyndon AR, Rothstein JD, Wyllie DJ, Tanaka K, Jackson M.
    • Journal Title

      Hum Mol Genet.

      Volume: 25 Pages: 4448-4461

    • DOI

      10.1093/hmg/ddw274

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effect of geranylgeranylacetone on the protection of retinal ganglion cells in a mouse model of normal tension glaucoma.2016

    • Author(s)
      Dong Z, Shinmei Y, Dong Y, Inafuku S, Fukuhara J, Ando R, Kitaichi N, Kanda A, Tanaka K, Noda K, Harada T, Chin S, Ishida S.
    • Journal Title

      Heliyon

      Volume: 2 Issue: 10 Pages: e00191-e00191

    • DOI

      10.1016/j.heliyon.2016.e00191

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ.2016

    • Author(s)
      Aida T, Nakade S, Sakuma T, Izu Y, Oishi A, Mochida K, Ishikubo H, Usami T, Aizawa H, Yamamoto T, Tanaka K.
    • Journal Title

      BMC Genomics

      Volume: 17 Issue: 1 Pages: 979-979

    • DOI

      10.1186/s12864-016-3331-9

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A CDC42EP4/septin-based perisynaptic glial scaffold facilitates glutamate clearance.2015

    • Author(s)
      Ageta-Ishihara N, Yamazaki M, Konno K, Nakayama H, Abe M, Hashimoto K, Nishioka T, Kaibuchi K, Hattori S, Miyakawa T, Tanaka K, Huda F, Hirai H, Hashimoto K, Watanabe M, Sakimura K, Kinoshita M.
    • Journal Title

      Nat Commun.

      Volume: 6 Issue: 1 Pages: 10090-10090

    • DOI

      10.1038/ncomms10090

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Neuronal heterotopias affect the activities of distant brain areas and lead to behavioral deficits2015

    • Author(s)
      Kazuhiro Ishii, Ken-ichiro Kubo, Toshihiro Endo, Keitaro Yoshida, Seico Benner, Yukiko Ito, Hidenori Aizawa, Michihiko Aramaki, Akihiro Yamanaka, Kohichi Tanaka, Norio Takata, Kenji Tanaka, Masaru Mimura, Chiahru Tohyama, Masaki Kakeyama, and Kazunori Nakajima
    • Journal Title

      Journal of Neuroscience

      Volume: 35 Issue: 36 Pages: 12432-45

    • DOI

      10.1523/jneurosci.3648-14.2015

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cloning-free CRISPR/Cas system facilitates functional cassette knockin in mice2015

    • Author(s)
      Aida T, Chiyo K, Usami T, Ishikubo H, Imahashi R, Wada Y, Tanaka K, Sakuma T, Yamamoto T, Tanaka K
    • Journal Title

      Genome Biol

      Volume: 16 Issue: 1 Pages: 87-87

    • DOI

      10.1186/s13059-015-0653-x

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Astroglial Glutamate Transporter Deficiency Increases Synaptic Excitability and Leads to Pathological Repetitive Behaviors in Mice.2015

    • Author(s)
      Aida T, Yoshida J, Nomura M, Tanimura A, Iino Y, Soma M, Bai N, Ito Y, Cui W, Aizawa H, Yanagisawa M, Nagai T, Takata N, Tanaka KF, Takayanagi R, Kano M, Gotz M, Hirase H, Tanaka K.
    • Journal Title

      Neuropsychopharmacology

      Volume: in press Issue: 7 Pages: 1660-1660

    • DOI

      10.1038/npp.2015.26

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Arundic acid attenuates retinal ganglion cell death by increasing glutamate/aspartate transporter expression in a model of normal tension glaucoma.2015

    • Author(s)
      Yanagisawa, M., Aida, T., Takeda, T., Namekata, K., Harada, T., Shinagawa, R. and *Tanaka. K.
    • Journal Title

      Cell Death and Disease

      Volume: 6 Issue: 3 Pages: e1693-e1693

    • DOI

      10.1038/cddis.2015.45

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Positive feedback of NR2B-containing NMDA receptor activity is the initial step toward visual imprinting: a model for juvenile learning.2015

    • Author(s)
      Nakamori T, Sato K, Kinoshita M, Kanamatsu T, Sakagami H, Tanaka K, Ohki-Hamazaki H.
    • Journal Title

      Journal of Neurochemistory

      Volume: 132 Issue: 1 Pages: 110-123

    • DOI

      10.1111/jnc.12954

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Valproic acid prevents retinal degeneration in a murine model of normal tension glaucoma2015

    • Author(s)
      Kimura, A., Guo, X., Noro, T., Harada, C., Tanaka, K., Namekata, K. and Harada, T
    • Journal Title

      Neuroscience Letters

      Volume: 588 Pages: 108-113

    • DOI

      10.1016/j.neulet.2014.12.054

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Glial dysfunction in the mouse habenula causes depressive-like behaviors and sleep disturbance.2014

    • Author(s)
      Cui W, Mizukami H, Yanagisawa M, Aida T, Nomura M, Isomura Y, Takayanagi R, Ozawa K, Tanaka K, and Aizawa H.
    • Journal Title

      J. Neurosci.

      Volume: 34 Issue: 49 Pages: 16273-16285

    • DOI

      10.1523/jneurosci.1465-14.2014

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] Loss of glial glutamate transporters induces motor neuron death in mice.2015

    • Author(s)
      Kaori Sugiyama, Tomomi Aida, Masatoshi Nomura, Ryoichi Takayanagi, Kohichi Tanaka
    • Organizer
      第38回日本分子生物学会
    • Place of Presentation
      神戸ポートアイランド(神戸市・兵庫県)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Neuronal heterotopias affect the activities of distant brain areas and lead to behavioral deficits2015

    • Author(s)
      Kazuhiro Ishii, Ken-ichiro Kubo1, Toshihiro Endo, Keitaro Yoshida, Seico Benner, Yukiko Ito, Hidenori Aizawa, Michihiko Aramaki, Akihiro Yamanaka, Kohichi Tanaka, Norio Takata, Kenji F. Tanaka, Masaru Mimura, Chiharu Tohyama, Masaki Kakeyama, and Kazunori Nakajima
    • Organizer
      Neuroscience 2105
    • Place of Presentation
      シカゴ(イリノイ州・米国)
    • Year and Date
      2015-10-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analyses of the pathological roles of the altered brain cytoarchitectures with ectopic neurons2015

    • Author(s)
      Ken-ichiro Kubo, Kazuhiro Ishii, Toshihiro Endo, Keitaro Yoshida, Seico Benner, Yukiko Ito, Hidenori Aizawa, Michihiko Aramaki, Akihiro Yamanaka, Kohichi Tanaka, Norio Takata, Kenji F. Tanaka, Masaru Mimura, Chiharu Tohyama, Masaki Kakeyama, and Kazunori Nakajima
    • Organizer
      第58回日本神経化学会大会
    • Place of Presentation
      大宮ソニックシティ(さいたま市・埼玉県)
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] Loss of glial glutamate transporter GLT-1 during development causes behavioral abnormalities relevant to the symptoms of schizophrenia2015

    • Author(s)
      Kaori Sugiyama, Daiki Nagaoka, Tomomi Aida, Kenji F Tanaka, Shigeyoshi Itohara, Kohichi Tanaka
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      神戸ポートアイランド(神戸市・兵庫県)
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Cloning-free genome editing in mice2015

    • Author(s)
      Kohichi Tanaka, Tomomi Aida, Keiho Chiyo, Takako Usami, Harumi Ishikubo, Risa Imahashi, Yusaku Wada, Kenji F. Tanaka, Tetsushi Sakuma, Takashi Yamamoto
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      神戸ポートアイランド(神戸市・兵庫県)
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] グルタミン酸トランスポーターGLASTのヒトレアバリアントは網膜神経節細胞の脆弱性にin vivoで寄与する2014

    • Author(s)
      今橋里沙、相田知海、柳澤美智子、佐久間哲史、宇佐美貴子、石久保春美、山本卓、田中光一
    • Organizer
      日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-11-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 機能カセットノックインマウスのためのCRISPR/Casを用いたin vivoゲノム編集2014

    • Author(s)
      相田知海、張景閎、佐久間哲史、宇佐美貴子、石久保春美、今橋里沙、田中謙二、山本卓、田中光一
    • Organizer
      日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-11-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] Glial dysfunction in the mouse habenula causes depressive behaviors and sleep disturbance2014

    • Author(s)
      Wangpeng Cui, Hiroaki Mizukami, Michiko Yanagisawa, Tomomi Aida, Masatoshi Nomura, Yoshikazu Isomura, Ryoichi Takayanagi, Keiya Ozawa, Kohichi Tanaka, Hidenori Aizawa
    • Organizer
      Annual Meeting of the Society for Neuroscience
    • Place of Presentation
      Washington, DC(米国)
    • Year and Date
      2014-11-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Enhancement of glutamate clearance via cortical glial glutamate transport (GLT-1) induced resiliency to stress2014

    • Author(s)
      Mouna Banasr, Ashley E Lepack, Jaime Maldonado-Aviles, Carly L Kiselycznyk, Vanja Duric, Jie Zhang, Stacey Wilber, Kohichi Tanaka, PRalph J DiLeone, Ronald S Duman, Gerard Sanacora
    • Organizer
      Annual Meeting of the Society for Neuroscience
    • Place of Presentation
      Washington, DC(米国)
    • Year and Date
      2014-11-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Deficiency of glutamate transporter in astrocyte increases brain excitability and induces Tourette’s Syndrome-like excessive repetitive behaviours in mice2014

    • Author(s)
      Tomomi Aida, Junichi Yoshida, Masatoshi Nomura, Asami Tanimura, Yusuke Iino, Miho Soma, Ning Bai, Yukiko Ito, Wangpeng Cui, Hidenori Aizawa, Terumi Nagai, Norio Takata, Ryoichi Takayanagi, Masanobu Kano, Magdalena Götz, Hajime Hirase, Kohichi Tanaka
    • Organizer
      Annual Meeting of the Society for Neuroscience
    • Place of Presentation
      Washington, DC(米国)
    • Year and Date
      2014-11-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Glial glutamate transporter GLT-1 determines sensitivity to the cortical spreading depression2014

    • Author(s)
      Hidenori Aizawa, Weinan Sun, Yukiko Ito, Saori Toyoda, Wangpeng Cui, Tomomi Aida, Masatoshi Nomura, Michiko Yanagisawa, Ryoichi Takayanagi, Kohichi Tanaka
    • Organizer
      Annual Meeting of the Society for Neuroscience
    • Place of Presentation
      Washington, DC(米国)
    • Year and Date
      2014-11-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] グルタミン酸トランスポーターのうつ様行動における役割2014

    • Author(s)
      相澤 秀紀、崔 万鵬、水上 浩明、柳澤 美智子、相田 知海、野村 政壽、礒村 宜和、高柳 涼一 、小澤 敬也、田中 光一
    • Organizer
      第37回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] グルタミン酸トランスポーターの欠損は過剰な繰り返し行動を引き起こす2014

    • Author(s)
      相田 知海、吉田 純一、野村 政壽、谷村 あさみ、飯野 祐介、相馬 美歩、Bai Ning、伊藤 亨子、Cui Wangpeng、相澤 秀紀、永井 てるみ、高田 則雄、髙栁 涼一、狩野 方伸、Magdalena Götz、平瀬 肇、田中 光一
    • Organizer
      第37回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] グリア型グルタミン酸トランスポーターGLT-1を前頭前野特異的に欠損したマウスは統合失調症様の行動異常を引き起こす2014

    • Author(s)
      橋本 真理子、相澤 秀紀、相田 知海、崔 万鵬、水上 浩明、小澤 敬也、野村 政壽、高柳 涼一、田中 光一
    • Organizer
      第37回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] グリア型グルタミン酸トランスポータGLT-1は皮質拡延性抑制に対する感受性を制御する2014

    • Author(s)
      豊田 早織、孫 偉楠、伊藤 亨子、崔 万鵬、相田 知海、柳澤 美智子、野村 政壽、高柳 涼一、田中 光一、相澤 秀紀
    • Organizer
      第37回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] 高速・高効率in vivoゲノム編集によるノックインマウス作出2014

    • Author(s)
      今橋 里沙、相田 知海、張 景ほう、佐久間 哲史、宇佐美 貴子、石久保 春美、Pawel Obrocki、山本 卓、田中 光一
    • Organizer
      第37回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] 脊髄におけるグリア型グルタミン酸トランスポーターの欠損はALS様症状を引き起こす2014

    • Author(s)
      杉山 香織、相田 知海、野村 政壽、高柳 涼一、田中 光一
    • Organizer
      第37回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] NR2B/NR1依存的な神経可塑的変化が幼少期の学習効率を上げている2014

    • Author(s)
      中森 智啓、佐藤 勝重、木下 雅恵、田中 光一、浜崎 浩子
    • Organizer
      第37回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] グリア型グルタミン酸トランスポーターGLT1の脳部位特異的機能解析2014

    • Author(s)
      杉本 潤哉、伊藤 亨子、相馬 美歩、崔 万鵬、三谷 章、野村 政壽、高柳 涼一、相澤 秀紀、田中 光一
    • Organizer
      第37回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Patent(Industrial Property Rights)] 簡便で高効率の遺伝子改変非ヒト哺乳動物の作製方法2014

    • Inventor(s)
      田中光一、相田知海、和田悠作
    • Industrial Property Rights Holder
      国立大学法人東京医科歯科大学、株式会社ファスマック
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-232963
    • Filing Date
      2014-11-17
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2022-02-16  

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