Functional analysis of Exoc1 gene related to embryo implamtation
Project/Area Number |
26290028
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Laboratory animal science
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Research Institution | University of Tsukuba |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
水野 聖哉 筑波大学, 医学医療系, 助教 (10633141)
杉山 文博 筑波大学, 医学医療系, 准教授 (90226481)
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Co-Investigator(Renkei-kenkyūsha) |
TAKAHASHI Satoru 筑波大学, 医学医療系, 教授 (50271896)
HOKAO Ryoji 動物繁殖研究所, 理事長 (80156992)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
|
Keywords | Exoc1 / マウス / 着床障害 / 自然突然変異 / 大規模ゲノム欠損 / 着床傷害 |
Outline of Final Research Achievements |
The homozygous WS mice, novel spontaneous mouse mutant with white spotting in the ventral body, showed peri-implantation lethal phenotype. Exoc1 gene was suggested as a prime candidate for this phenotype. We produced Exoc1 knockout mice, and the same peri-implantation lethal phenotype was observed in Exoc1-/- embryos. In addition, the polygenic effect without Exoc1 was investigated in genome-edited mutant mice carrying the 1.02 Mb deletion allele (the region from Kit to just upstream of Exoc1) in mice using the CRISPR/Cas9 system. This introduced mutation did not affect early embryonic development. These data indicate that Exoc1, which is located in the vicinity of the Kit gene, is the monogenic causative gene for peri-implantation lethality in the homozygous WS mice.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Generation of CRISPR/Cas9-mediated bicistronic knock-in <i>ins1-cre</i> driver mice2016
Author(s)
Yoshikazu Hasegawa, Yoshikazu Hoshino, Abdelaziz E. Ibrahim, Kanako Kato, Yoko Daitoku1, Yoko Tanimoto, Yoshihisa Ikeda, Hisashi Oishi, Satoru Takahashi, Atsushi Yoshiki, Ken-ichi Yagami, Hiroyoshi Iseki, Seiya Mizuno, Fumihiro Sugiyama
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Journal Title
Experimental Animals
Volume: 65
Issue: 3
Pages: 319-327
DOI
NAID
ISSN
0007-5124, 1341-1357, 1881-7122
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Germline recombination in a novel Cre transgenic line, Prl3b1-cre mouse.2016
Author(s)
Al-Soudy AS, Hiromori Y, Mizuno S, Hasegawa Y, Shawki HH, Katoh MC, Basha WA, Ibrahim AE, El-Shemy HA, Iseki Hi, Yoshiki A, Nagase H, Nakanishi T, Takahashi S, Oishi H, Sugiyama F.
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Journal Title
Genesis.
Volume: 54(7)
Issue: 7
Pages: 389-97
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] Applied Genome Editing with CRISPR/Cas9 Plasmid in Mice2015
Author(s)
Seiya Mizuno, Kanako Kato, Saori Iijima, Yuko Hamada, Tra Dinh Thi Huong, Yoshikazu Hoshino, Yoko Tanimoto, Satoru Takahashi, Fumihiro Sugiyama, Ken-ichi Yagami
Organizer
トランスポゾン転移とゲノム編集技術に関する国際会議2015
Place of Presentation
奈良県新公会堂(奈良市)
Year and Date
2015-11-17
Related Report
Int'l Joint Research
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