Development of genome editing technologies in laboratory rats
Project/Area Number |
26290033
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Laboratory animal science
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Research Institution | Osaka University (2015-2017) Kyoto University (2014) |
Principal Investigator |
Mashimo Tomoji 大阪大学, 医学系研究科, 准教授 (80397554)
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Co-Investigator(Kenkyū-buntansha) |
金子 武人 岩手大学, 理工学部, 准教授 (30332878)
夘野 善弘 大阪大学, 医学系研究科, 助手 (80252683)
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
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Keywords | ゲノム編集 / ラット / 遺伝子 / 脳神経疾患 / 実験動物 |
Outline of Final Research Achievements |
In order to develop novel genome editing technologies in laboratory rats, we succeeded to generate genome-edited rats, such as knockouts, SNP exchange, a dozen bp insertion, and several Kbp deletion, using the CRISPR/Cas9 system. We also developed a GFP cassette knock-ins using long single-stranded DNA (lssDNA), a CLICK method to efficiently generate conditional knockout rats, a 2H2OP method to knock-in large scale genomic regions such as 200 Kb BAC. The 2H2OP method and the CLICK method were patented. We are developing in vivo genomic editing technology using Cas9-transgenic rats, as well as are generating new rat models of Parkinson's disease using the rat genome editing technology we established in this study.
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Report
(5 results)
Research Products
(100 results)
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[Journal Article] Rats deficient C-type natriuretic peptide suffer from impaired skeletal growth without early death.2018
Author(s)
Fujii T, Hirota K, Yasoda A, Takizawa A, Morozumi N, Nakamura R, Yotsumoto T, Kondo E, Yamashita Y, Sakane Y, Kanai Y, Ueda Y, Yamauchi I, Yamanaka S, Nakao K, Kuwahara K, Jindo T, Furuya M, Mashimo T, Inagaki N, Serikawa T, Nakao K.
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Journal Title
PROS ONE
Volume: 13
Issue: 3
Pages: e0194812-e0194812
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] A rat model of ataxia-telangiectasia: evidence for a neurodegenerative phenotype.2017
Author(s)
Quek H, Luff J, Cheung K, Kozlov S, Gatei M, Lee CS, Bellingham MC, Noakes PG, Lim YC, Barnett NL, Dingwall S, Wolvetang E, Mashimo T, Roberts TL, Lavin MF.
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Journal Title
Human Molecular Genetics
Volume: 26(1)
Pages: 109-123
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Rats with a missense mutation in Atm display neuroinflammation and neurodegeneration subsequent to accumulation of cytosolic DNA following unrepaired DNA damage.2016
Author(s)
Quek H, Luff J, Cheung K, Kozlov S, Gatei M, Lee CS, Bellingham MC, Noakes PG, Lim YC, Barnett NL, Dingwall S, Wolvetang E, Mashimo T, Roberts TL, Lavin MF.
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Journal Title
Journal of Leukocyte Biology
Volume: -
Issue: 4
Pages: 927-947
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Synaptic vesicle glycoprotein 2A (SV2A) regulates kindling epileptogenesis via GABAergic neurotransmission.2016
Author(s)
Tokudome K, Okumura T, Shimizu S, Mashimo T, Takizawa A, Serikawa T, Terada R, Ishihara S, Kunisawa N, Sasa M, Ohno Y.
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Journal Title
Scientific Reports
Volume: 6
Issue: 1
Pages: 2742-2742
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Depdc5 knockout rat: A novel model of mTORopathy.2016
Author(s)
Marsan E, Ishida S, Schramm A, Weckhuysen S, Muraca G, Lecas S, Liang N, Treins C, Pende M, Roussel D, Le Van Quyen M, Mashimo T, Kaneko T, Yamamoto T, Sakuma T, Mahon S, Miles R, Leguern E, Charpier S, Baulac S.
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Journal Title
Neurobiology of Disease
Volume: 9;89
Pages: 180-189
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] ・Seipin is necessary for normal brain development and spermatogenesis in addition to adipogenesis.2015
Author(s)
Ebihara C, Ebihara K, Aizawa-Abe M, Mashimo T, Tomita T, Zhao M, Gumbilai VM, Kusakabe T, Yamamoto Y, Aotani D, Yamamoto-Kataoka S, Sakai T, Hosoda K, Serikawa T, Nakao K
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Journal Title
Hum Mol Genet
Volume: 24
Issue: 15
Pages: 4238-4249
DOI
Related Report
Peer Reviewed
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[Presentation] ゲノム編集とは2015
Author(s)
真下知士
Organizer
ヒトゲノム解析研究倫理審査を考える会
Place of Presentation
千里ライフサイエンスセンター(大阪府豊中市)
Year and Date
2015-08-09
Related Report
Invited
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