| Project/Area Number |
26290033
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Osaka University (2015-2017)
Kyoto University (2014) |
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Principal Investigator |
Mashimo Tomoji 大阪大学, 医学系研究科, 准教授 (80397554)
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| Co-Investigator(Kenkyū-buntansha) |
金子 武人 岩手大学, 理工学部, 准教授 (30332878)
夘野 善弘 大阪大学, 医学系研究科, 助手 (80252683)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
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| Keywords | ゲノム編集 / ラット / 遺伝子 / 脳神経疾患 / 実験動物 |
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| Outline of Final Research Achievements |
In order to develop novel genome editing technologies in laboratory rats, we succeeded to generate genome-edited rats, such as knockouts, SNP exchange, a dozen bp insertion, and several Kbp deletion, using the CRISPR/Cas9 system. We also developed a GFP cassette knock-ins using long single-stranded DNA (lssDNA), a CLICK method to efficiently generate conditional knockout rats, a 2H2OP method to knock-in large scale genomic regions such as 200 Kb BAC. The 2H2OP method and the CLICK method were patented. We are developing in vivo genomic editing technology using Cas9-transgenic rats, as well as are generating new rat models of Parkinson's disease using the rat genome editing technology we established in this study.
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