Elucidation of functions of telomere-adjacent regions
Project/Area Number |
26290061
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Genome biology
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Research Institution | Osaka University |
Principal Investigator |
Kanoh Junko 大阪大学, たんぱく質研究所, 准教授 (10323809)
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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Keywords | 染色体 / テロメア / サブテロメア / クロマチン / DNA複製 / 遺伝子発現 / ヘテロクロマチン |
Outline of Final Research Achievements |
Functions of subtelomeres, particularly subtelomeric homologous (SH) sequences, remain elusive. Complete removal of SH sequences from the genome revealed that they are dispensable for mitosis, meiosis, and telomere length control. However, SH sequences prevent deleterious inter-chromosomal end fusion by facilitating intra-chromosomal circularization. Surprisingly, SH-deleted cells sometimes survive telomere loss through inter-chromosomal end fusions via homologous loci such as LTRs, accompanied by centromere inactivation of either chromosome. Moreover, SH sequences function as a buffer region against the spreading of subtelomeric heterochromatin into the neighboring gene-rich regions. Furthermore, we found a nucleosome-free region at the subtelomeric border, which may be a second barrier that blocks heterochromatin spreading into the subtelomere-adjacent euchromatin. Thus, our results demonstrate multiple defense functions of subtelomeres in chromosome homeostasis and gene expression.
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Report
(5 results)
Research Products
(41 results)
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[Journal Article] CK2 phospho-independent assembly of the Tel2-associated stress-signaling complexes in Schizosaccharomyces pombe.pendent2017
Author(s)
Inoue, H., Sugimoto, S., Takeshita, Y., Takeuchi, M., Hatanaka, M., Nagao, K., Hayashi, T., Kokubu, A., Yanagida, M., and Kanoh, J..,
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Journal Title
Genes Cells
Volume: 22
Issue: 1
Pages: 59-70
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Shugoshin forms a specialized chromatin domain at subtelomeres that regulates transcription and replication timing.2016
Author(s)
Tashiro S, Handa T, Matsuda A, Ban T, Takigawa T, Miyasato K, Ishii K, Kugou K, Ohta K, Hiraoka Y, Masukata H, Kanoh J.
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Journal Title
Nature Commun.
Volume: 7
Issue: 1
Pages: 10393-10393
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Fission yeast telomere-binding protein Taz1 is a functional but not a structural counterpart of human TRF1 and TRF2.2015
Author(s)
Deng, W., Wu, J., Wang, F., Kanoh, J., Dehe, PM., Inoue, H., Chen, J., and Lei, M.
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Journal Title
Cell Research
Volume: 25
Issue: 7
Pages: 881-884
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] サブテロメアの機能解明2017
Author(s)
田代三喜、西原祐輝、久郷和人、太田邦史、加納純子
Organizer
ワークショップ「染色体研究の最前線」
Place of Presentation
大阪大学(大阪, 吹田市)
Related Report
Invited
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[Presentation] サブテロメアの機能を探る2015
Author(s)
加納純子
Organizer
クロマチンデコーディング2015年度第1回研究会
Place of Presentation
国際高等研究所
Year and Date
2015-12-19
Related Report
Invited
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[Presentation] サブテロメアの新規機能の解明2015
Author(s)
Tashiro, S., Handa, T., Matsuda, A., Ban, T., Takigawa, T., Miyasato, K., Ishii, K., Kugou, K., Ohta, K., Hiraoka, Y., Masukata, H., and Kanoh, J.
Organizer
BMB2015
Place of Presentation
神戸ポートアイランド、神戸
Year and Date
2015-12-01
Related Report
Invited
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[Presentation] Shugoshin forms a specialized chromatin domain at subtelomeres that regulates transcription and replication timing.2015
Author(s)
Tashiro, S., Handa, T., Matsuda, A., Ban, T., Takigawa, T., Miyasato, K., Ishii, K., Kugou, K., Ohta, K., Hiraoka, Y., Masukata, H., and Kanoh, J.
Organizer
第8回国際分裂酵母会議
Place of Presentation
生田神社会館、神戸
Year and Date
2015-06-21
Related Report
Int'l Joint Research / Invited
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[Presentation] Spindle assembly checkpoint protein Sgo2 regulates silenced chromatin formation and DNA replication timing at subtelomeres2015
Author(s)
Tashiro, S., Handa, T., Matsuda, A., Ban, T., Takigawa, T., Miyasato, K., Ishii, K., Kugou, K., Ohta, K., Hiraoka, Y., Masukata, H., and Kanoh, J.
Organizer
Telomeres and telomerase, Cold Spring Harbor Laboratory meeting
Place of Presentation
Cold Spring Harbor Laboratory, NY, USA
Year and Date
2015-04-28
Related Report
Int'l Joint Research
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[Presentation] 染色体末端の神秘2014
Author(s)
加納純子
Organizer
第21回酵母合同シンポジウム
Place of Presentation
東京大学、東京
Year and Date
2014-09-03 – 2014-09-04
Related Report
Invited
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