| Project/Area Number |
26290071
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
System genome science
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Furusawa Chikara 国立研究開発法人理化学研究所, 生命システム研究センター, チームリーダー (00372631)
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| Co-Investigator(Kenkyū-buntansha) |
堀之内 貴明 国立研究開発法人理化学研究所, 生命システム研究センター, 特別研究員 (60610988)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Keywords | 進化実験 / 抗生物質耐性 / 大腸菌 / 抗生物質 / 次世代シーケンサ |
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| Outline of Final Research Achievements |
The emergence of multidrug-resistant bacteria is a growing concern for global public health. To suppress the emergence of antibiotic resistant bacteria, it is important to understand its evolutionary dynamics. In this study, we performed laboratory evolution of Escherichia coli under addition of two antibiotics and analyzed its evolutionary dynamics. As a result, we identified drug combinations by which resistance acquisition was suppressed and accelerated. In addition, to screen stress environment which can cause suppression or acceleration of drug-resistant evolution, we performed laboratory evolution of E. coli under various stress conditions, including an addition of acid, alkali, and so forth. As results, we successfully identified some candidate for the suppression of drug resistance evolution. These results provide the basis for controlling antibiotic resistance evolution.
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