Molecular mechanism of lncRNA-mediated architecture of subnuclear structures

• Project/Area Number: 26291001
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Hokkaido University
• Principal Investigator: Hirose Tetsuro 北海道大学, 遺伝子病制御研究所, 教授 (30273220)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000); Fiscal Year 2016: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
• Keywords: 非コードRNA / RNA結合タンパク質 / 核内構造体 / 生体分子 / 核酸 / 発現制御 / 細胞 / ゲノム / ノンコーディングRNA / タンパク質凝集

Outline of Final Research Achievements

The roles of the protein factors that compose lncRNA-dependent nuclear bodies were intensively and pleiotropically studied. First, the SWI/SNF complexes were identified as novel essential factors for nuclear body formation with their novel assembly activity distinct from the characterized chromatin remodeling activity. Second, the prion-like domain (PLD) of RNA binding proteins that are essential for nuclear body formation was shown to be required for nuclear body formation. It was also detected that the PLD can form the hydrogel structure in vitro, suggesting this feature to form hydrogel underlies the mechanism of the structural roles of these proteins in nuclear body assembly. Finally, we discovered unusual semi-extractable feature of the nuclear body forming lncRNAs. It was shown that the PLD of RNA-binding proteins are responsible for the semi-extractable feature of this class of lncRNAs. We also developed the new method to efficiently extract these RNAs from the cells.

Research Products

• [Int'l Joint Research] CNRS(フランス)
• [Int'l Joint Research] 西オーストラリア大(オーストラリア)
• [Int'l Joint Research] KAUST(サウジアラビア)
• [Int'l Joint Research] CNRS(フランス)
• [Int'l Joint Research] 西オーストラリア大学(オーストラリア)
• [Journal Article] RNase sensitivity screening for nuclear bodies with RNA scaffolds in mammalian cells. (2017)
  • Author(s): Mannen T, Hirose T
  • Journal Title: Bio-protocol Volume: -
  • Peer Reviewed
• [Journal Article] Unusual semi‐extractability as a hallmark of nuclear body‐associated architectural noncoding RNAs (2017)
  • Author(s): Chujo T, Yamazaki T, Kawaguchi T, Kurosaka S, Takumi T, Nakagawa S, Hirose T
  • Journal Title: The EMBO Journal Volume: - Issue: 10 Pages: 861-868
  • DOI: 10.15252/embj.201695848
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] p53 induces formation of NEAT1 lncRNA-containing paraspeckles that modulate replication stress response and chemosensitivity. (2016)
  • Author(s): Adriaens C, Standaert L, Barra J, Latil M, Verfaillie A, Kalev P, Boeckx B, Wijnhoven PWG, Radaelli E, Vermi W, Leucci E, Lapouge G, Beck B, ven den Oord J, Nakagawa S, Hirose T, Sablina AA, Lambrechts D, Aerts S, Blanpain C, Marine JC.
  • Journal Title: Nat. Med. Volume: 22 Issue: 8 Pages: 861-868
  • DOI: 10.1038/nm.4135
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The Sam68 nuclear body is composed of two RNase-sensitive substructures joined by the adaptor HNRNPL (2016)
  • Author(s): Mannen T, Yamashita S, Tomita K, Goshima G, Hirose T
  • Journal Title: J Cell Biol. Volume: 214 Issue: 1 Pages: 45-59
  • DOI: 10.1083/jcb.201601024
  • NAID: 120005828468
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Control of the heat stress-induced alternative splicing of a subset of genes by hnRNP K. (2016)
  • Author(s): Yamamoto K, Furukawa M, Fukumura K, Kawamura A, Yamada T, Suzuki H, Hirose T, Sakamoto H, Inoue K.
  • Journal Title: Genes Cells. Volume: 21 Issue: 9 Pages: 1006-1014
  • DOI: 10.1111/gtc.12400
  • Peer Reviewed
• [Journal Article] GAS5 Gene (2016)
  • Author(s): Aly, MK, Hirose T
  • Journal Title: Encyclopedia of Life Sciences Volume: Epub Pages: 1-7
  • DOI: 10.1002/9780470015902.a0005019.pub3
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Regulation of membrane KCNQ1/KCNE1 channel density by sphingomyelin synthase 1. (2016)
  • Author(s): Wu M, Takemoto M, Taniguchi M, Takumi T, Okazaki T, Song WJ
  • Journal Title: Am J Physiol Cell Physiol Volume: 311 Issue: 7 Pages: 817-830
  • DOI: 10.1083/jcb.201601071
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Paraspeckles modulate the intranuclear distribution of paraspeckle-associated Ctn RNA. (2016)
  • Author(s): Anantharaman A, Jadaliha M, Tripathi V, Nakagawa S, Hirose T, Jantsch MF, Prasanth SG, Prasanth KV.
  • Journal Title: Sci Rep Volume: 6 Issue: 1 Pages: 34043-34043
  • DOI: 10.1038/srep34043
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Architectural RNAs (arcRNAs): A class of long noncoding RNAs that functions as the scaffold of nuclear bodies (2016)
  • Author(s): Takeshi Chujo, Tomohiro Yamazaki, Tetsuro Hirose
  • Journal Title: Biochim Biophys Acta Volume: 1859 Issue: 1 Pages: 139-146
  • DOI: 10.1016/j.bbagrm.2015.05.007
  • Peer Reviewed
• [Journal Article] SWI/SNF chromatin-remodeling complexes function in noncoding RNA-dependent assembly of nuclear bodies. (2015)
  • Author(s): Kawaguchi T, Tanigawa A, Naganuma T, Ohkawa Y, Souquere S, Pierron G, Hirose T.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 112 Issue: 14 Pages: 4304-4309
  • DOI: 10.1073/pnas.1423819112
  • NAID: 120005661435
  • Peer Reviewed / Open Access
• [Journal Article] Prion-like domains in RNA binding proteins are essential for building subnuclear paraspeckles. (2015)
  • Author(s): Hennig S, Kong G, Mannen T, Sadowska A, Kobelke S, Blythe A, Knott G, Iyer S, Ho D, Newcombe EA, Hosoki K, Goshima N, Hatters D, Trinkle-Mulcahy L, Hirose T*, Bond CS*, Fox AH* (*co-corresponding)
  • Journal Title: J. Cell Biol. Volume: 210 Issue: 4 Pages: 3529-539
  • DOI: 10.1083/jcb.201504117
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Chromatin remodeling complexes in the assembly of long noncoding RNA-dependent nuclear bodies. (2015)
  • Author(s): Kawaguchi T, Hirose T.
  • Journal Title: Nucleus Volume: 6 Issue: 6 Pages: 462-467
  • DOI: 10.1080/19491034.2015.1119353
  • NAID: 120005905615
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Clues to long noncoding RNA taxonomy (2015)
  • Author(s): Hirose T, Nakagawa S.
  • Journal Title: Biochim Biophys Acta. Volume: 1859 Pages: 1-2
  • Peer Reviewed
• [Journal Article] The building process of the functional paraspeckle with long non-coding RNAs. (2015)
  • Author(s): Yamazaki, T., Hirose, T.
  • Journal Title: Front. Biosci. Volume: 7 Pages: 1-47
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Genome-wide co-localization screening of nuclear body components using a fluorescently tagged FLJ cDNA clone library. (2015)
  • Author(s): Hirose, T., Goshima, N.
  • Journal Title: Methods Mol. Biol. Volume: 1262 Pages: 155-163
  • DOI: 10.1007/978-1-4939-2253-6_9
  • ISBN: 9781493922529, 9781493922536
  • Peer Reviewed
• [Journal Article] Rapid and Efficient Elimination of Specific Nuclear Noncoding RNAs in Mammalian Cells with Antisense Oligonucleotides. (2015)
  • Author(s): Hirose, T., Mannen, T.
  • Journal Title: Methods Mol. Biol. Volume: 1206 Pages: 149-156
  • DOI: 10.1007/978-1-4939-1369-5_13
  • ISBN: 9781493913688, 9781493913695
  • Peer Reviewed
• [Journal Article] The lncRNA Neat1 is required for corpus luteum formation and the establishment of pregnancy in a subpopulation of mice. (2014)
  • Author(s): Nakagawa S, Shimada M, Yanaka K, Mito M, Arai T, Takahashi E, Fujita Y, Fujimori T, Standaert L, Marine JC, Hirose T.
  • Journal Title: Development Volume: 141 Issue: 23 Pages: 4618-27
  • DOI: 10.1242/dev.110544
  • Peer Reviewed / Open Access
• [Journal Article] The long noncoding RNA Neat1 is required for mammary gland development and lactation. (2014)
  • Author(s): Standaert, L., Adriaens, C., Radaelli, E., Van Keymeulen, A., Blanpain, C., Hirose, T., Nakagawa, S. & Marine, J.C.
  • Journal Title: RNA Volume: 20 Issue: 12 Pages: 1844-1849
  • DOI: 10.1261/rna.047332.114
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The Estrogen receptor alpha regulated lncRNA NEAT1 is a critical modulator of prostate cancer. (2014)
  • Author(s): Chakravarty, D., Sboner, A., Nair, S., Giannopoulou, E., Li, R., Hennig, S., Mosquera, J.M., Pauwels, J., Park, K., Kossai, M., MacDonald, T., Fontugne, J., Erho, N., Vergara, I., Ghadessi, M., Davicioni, E., Jenkins, R., Palanisamy, N., Chen, Z., Nakagawa, S., Hirose, T., et al.
  • Journal Title: Nat. Commun. Volume: 5 Issue: 1 Pages: 5383-5383
  • DOI: 10.1038/ncomms6383
  • Peer Reviewed
• [Journal Article] Elements and machinery of non-coding RNAs: toward their taxonomy (2014)
  • Author(s): Tetsuro Hirose, Yuichiro Mishima, Yukihide Tomari
  • Journal Title: EMBO Reports Volume: 印刷中 Issue: 5 Pages: 489-507
  • DOI: 10.1002/embr.201338390
  • Peer Reviewed / Open Access
• [Presentation] NEAT1 lncRNAに潜むパラスペックル構築を司るRNAエレメント (2017)
  • Author(s): 山崎智弘, Sylvie Souquere、木立尚孝、Archa H. Fox, 中川真一, Gerard Pierron, 廣瀬哲郎
  • Organizer: 第２回北大・部局横断シンポジウム「免疫・癌・感染」
  • Place of Presentation: 北海道大学工学部鈴木章記念ホール
  • Year and Date: 2017-03-14
• [Presentation] Novel functional insights into Sat III lncRNA-dependent nuclear stress bodies in post-stress recovery phase. (2017)
  • Author(s): 二宮 賢介、Mahmoud Khamis Aly、廣瀬 哲郎
  • Organizer: 第２回北大・部局横断シンポジウム「免疫・癌・感染」
  • Place of Presentation: 北海道大学工学部鈴木章記念ホール
  • Year and Date: 2017-03-14
• [Presentation] Unusual semi-extractability as the hallmark of nuclear body associated architectural long noncoding RNAs (2017)
  • Author(s): 中條岳志，山崎智弘，川口哲哉，黒坂哲，内匠透，中川真一，廣瀬哲郎
  • Organizer: 第２回北大・部局横断シンポジウム「免疫・癌・感染」
  • Place of Presentation: 北海道大学工学部鈴木章記念ホール
  • Year and Date: 2017-03-14
• [Presentation] Distinct RNA polymerase transcripts competitively function as scaffold of the DBC1 nuclear bodies in specific cancer cell lines. (2017)
  • Author(s): Taro Mannen, Seisuke Yamashita, Kozo Tomita, Naoki Goshima, Tetsuro Hirose.
  • Organizer: 第２回北大・部局横断シンポジウム「免疫・癌・感染」
  • Place of Presentation: 北海道大学工学部鈴木章記念ホール
  • Year and Date: 2017-03-14
• [Presentation] Unravelling The Architectural Organization of The SAT III lncRNA- Dependent Nuclear Stress Bodies (2017)
  • Author(s): Mahmoud Khamis Aly, Kensuke Ninomiya, Shungo Adachi, Tohru Natsume and Tetsuro Hirose
  • Organizer: 第２回北大・部局横断シンポジウム「免疫・癌・感染」
  • Place of Presentation: 北海道大学工学部鈴木章記念ホール
  • Year and Date: 2017-03-14
• [Presentation] Architectural lncRNAの機能解明 (2017)
  • Author(s): 廣瀬哲郎
  • Organizer: バイオシグナル研究会
  • Place of Presentation: 神戸大学（兵庫県神戸市）
  • Year and Date: 2017-03-08
  • Invited
• [Presentation] Liquid-liquid phase separation induced by NEAT1 lncRNA that builds paraspeckle nuclear body. (2017)
  • Author(s): Tomohiro Yamazaki、Sylvie Souquere, Archa H. Fox, Shinichi Nakagawa, Gerard Pierron, Tetsuro Hirose.
  • Organizer: Phase Separation and RNA Processing as Drivers of Cancer and Neurodegenerative Disease.
  • Place of Presentation: Sanford Consortium(San Diego, CA, USA)
  • Year and Date: 2017-02-24
  • Int'l Joint Research
• [Presentation] Dissection of architectural noncoding RNA elements and machinery (2017)
  • Author(s): Tetsuro Hirose
  • Organizer: Simon Fraser Univ MBB seminar
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 2017-02-10
  • Int'l Joint Research / Invited
• [Presentation] Liquid-liquid phase separation and paraspeckle formation induced by the specific region of NEAT1 lncRNA (2017)
  • Author(s): Tetsuro Hirose
  • Organizer: Keystone Symposia
  • Place of Presentation: Banff, Canada
  • Year and Date: 2017-02-07
  • Int'l Joint Research
• [Presentation] Remarkable semi-extractability as the hallmark of nuclear body-associated architectural long ncRNAs (2017)
  • Author(s): Takeshi Chujo, Tomohiro Yamazaki, Tetsuya Kawaguchi, Satoshi Kurosaka,Toru Takumi, Shinichi Nakagawa, and Tetsuro Hirose.
  • Organizer: Keystone Symposia Protein-RNA Interactions: Scale, Mechanisms, Structure and Function of Coding and Noncoding RNPs
  • Place of Presentation: Banff （Canada）
  • Year and Date: 2017-02-07
  • Int'l Joint Research
• [Presentation] Distinct RNA polymerase transcripts competitively function as scaffold of the DBC1 nuclear bodies in specific cancer cell lines (2017)
  • Author(s): Taro Mannen, Seisuke Yamashita, Kozo Tomita, Naoki Goshima, Tetsuro Hirose.
  • Organizer: 第11回研究所ネットワーク国際シンポジウム
  • Place of Presentation: 徳島大学藤井節郎記念ホール（徳島県蔵本町）
  • Year and Date: 2017-01-26
• [Presentation] Characterization of architectural lncRNA elements using genome-editing technology. (2016)
  • Author(s): 廣瀬哲郎
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-12-01
  • Invited
• [Presentation] RNA elements in NEAT1 lncRNA for the architectural role of the paraspeckle (2016)
  • Author(s): 山崎 智弘、Sylvie Souquere、木立尚孝、馬場由貴、麦倉一樹、Archa H. Fox、Charles S. Bond、中川 真一、Gerard Pierron、廣瀬 哲郎
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-11-30
• [Presentation] Sam68核内構造体は、HNRNPLアダプターを介して2つのRNase感受性サブ構造体が融合してつくられる (2016)
  • Author(s): 萬年太郎, 山下征輔, 富田耕造, 五島直樹, 廣瀬哲郎
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-11-30
• [Presentation] Remarkable semi-extractability as a hallmark of architectural long noncoding RNAs associated with nuclear bodies (2016)
  • Author(s): Takeshi Chujo, Tomohiro Yamazaki, Tetsuya Kawaguchi, Satoshi Kurosaka, Toru Takumi, Shinichi Nakagawa, Tetsuro Hirose
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-11-30
• [Presentation] Dissection of architectural noncoding RNA elements and machinery. (2016)
  • Author(s): 廣瀬哲郎
  • Organizer: Cold Spring Harbor Aisa meeting
  • Place of Presentation: World Hotel Grand Dushulake Suzhou（中国 蘇州）
  • Year and Date: 2016-11-17
  • Int'l Joint Research / Invited
• [Presentation] Elements and machinery of architectural long noncoding RNAs. (2016)
  • Author(s): 廣瀬哲郎
  • Organizer: Shanghai Institutes for Biological Sciences Seminar series
  • Place of Presentation: Shanghai Institute for Biological Science（中国 上海）
  • Year and Date: 2016-11-14
  • Int'l Joint Research / Invited
• [Presentation] プリオン様RNA結合タンパク質の機能制御を担うnon-coding RNA作動エレメントの解析 (2016)
  • Author(s): 廣瀬哲郎
  • Organizer: 「筋萎縮性側索硬化症（ALS）新規治療法開発をめざした病態解明」 平成28年度ワークショップ
  • Place of Presentation: 都市センターホテル（東京都千代田区）
  • Year and Date: 2016-09-30
  • Invited
• [Presentation] Architectural noncoding RNAs controlling subnuclear organization and gene expression. (2016)
  • Author(s): 廣瀬哲郎
  • Organizer: Mini-symposium on ncRNA
  • Place of Presentation: Academia Sinica （台湾台北市）
  • Year and Date: 2016-09-29
  • Int'l Joint Research / Invited
• [Presentation] Functional characterization of architectural RNA species toward noncoding RNA taxonomy. (2016)
  • Author(s): 廣瀬哲郎
  • Organizer: RNA2016 Symposium
  • Place of Presentation: 国立成功大学（台湾台南市）
  • Year and Date: 2016-09-27
  • Int'l Joint Research / Invited
• [Presentation] 核内構造体の機能構築に必要なlncRNA機能ドメインの解析 (2016)
  • Author(s): 廣瀬哲郎
  • Organizer: 第89回日本生化学会大会シンポジウム
  • Place of Presentation: 東北大学（宮城県仙台市）
  • Year and Date: 2016-09-25
  • Invited
• [Presentation] Remarkable semi-extractability as a hallmark of architectural long noncoding RNAs associated with nuclear bodies (2016)
  • Author(s): Takeshi Chujo, Tomohiro Yamazaki, Tetsuya Kawaguchi, Satoshi Kurosaka, Toru Takumi, Shinichi Nakagawa, Tetsuro Hirose
  • Organizer: RNAフロンティアミーティング2016
  • Place of Presentation: いこいの湯宿いろは（北海道ニセコ町）
  • Year and Date: 2016-08-31
• [Presentation] 非コードRNAの配列と機能の連関 (2016)
  • Author(s): 廣瀬哲郎
  • Organizer: 日本進化学会第18回大会
  • Place of Presentation: 東京工業大学（東京都目黒区）
  • Year and Date: 2016-08-25
  • Invited
• [Presentation] Decoding “architectural” RNA elements in NEAT1 lncRNA (2016)
  • Author(s): Tomohiro Yamazaki, Sylvie Souquere, Archa H. Fox, Gerard Pierron, Shinichi Nakagawa, Tetsuro Hirose
  • Organizer: RNA 2016
  • Place of Presentation: 国立京都国際会館（京都府京都市）
  • Year and Date: 2016-06-28
  • Int'l Joint Research
• [Presentation] The Sam68 nuclear body is composed of two RNase-sensitive substructures with the adaptor HNRNPL (2016)
  • Author(s): Taro Mannen, Naoki Goshima, and Tetsuro Hirose
  • Organizer: RNA2016
  • Place of Presentation: 国立京都国際会館（京都府京都市）
  • Year and Date: 2016-06-28
  • Int'l Joint Research
• [Presentation] Semi-extractable feature as the hallmark of nuclear body-associated architectural long ncRNAs (2016)
  • Author(s): 廣瀬哲郎
  • Organizer: 国際シンポジウム「Clues to Non-coding RNA Taxonomy」
  • Place of Presentation: 東京大学（東京都文京区）
  • Year and Date: 2016-06-27
  • Invited
• [Presentation] Re-quantification of paraspeckle architectural components and an exploratory search for novel nuclear RNA bodies by using an improved RNA extraction method. (2016)
  • Author(s): Takeshi Chujo, Tomohiro Yamazaki, Shinichi Nakagawa, Tetsuya Kawaguchi, Chikako Fujikawa, Ayaka Kubota, Toru Takumi, Tetsuro Hirose
  • Organizer: Keystone symposia
  • Place of Presentation: Santa Fe, USA
  • Year and Date: 2016-02-21
  • Int'l Joint Research
• [Presentation] CRISPR/Cas9-mediated dissection of NEAT1 lncRNA to identify “architectural” RNA elements. (2016)
  • Author(s): Tomohiro Yamazaki and Tetsuro Hirose
  • Organizer: Keystone symposia
  • Place of Presentation: Santa Fe, USA
  • Year and Date: 2016-02-21
  • Int'l Joint Research
• [Presentation] NEAT1 lncRNAの難溶性を考慮したパラスペックル構造因子の再定量と構造構築原理の探究 (2015)
  • Author(s): 中條 岳志、中川 真一、川口哲哉、内匠 透、廣瀬 哲郎
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] RNase感受性スクリーニングにより同定された核内RNA顆粒の形成機構の解析 (2015)
  • Author(s): 萬年 太郎、五島 直樹、廣瀬 哲郎
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] CRISPR/Cas9-mediated dissection of NEAT1 lncRNA to identify “architectural” RNA elements (2015)
  • Author(s): Tomohiro Yamazaki and Tetsuro Hirose
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] Decoding noncoding RNA code: 細胞内構造構築機能を中心に (2015)
  • Author(s): 廣瀬哲郎
  • Organizer: 国際医療センター転写研究会セミナー
  • Place of Presentation: 国立国際医療研究センター（東京都新宿区）
  • Year and Date: 2015-11-18
  • Invited
• [Presentation] ncRNA研究アップデート2015 (2015)
  • Author(s): 廣瀬哲郎
  • Organizer: バイオファイナンスギルド
  • Place of Presentation: バイオフロンティアパートナーズ特別講堂（東京都中央区）
  • Year and Date: 2015-11-12
  • Invited
• [Presentation] Long noncoding RNA による核内構造の動態制御 (2015)
  • Author(s): 廣瀬哲郎
  • Organizer: 臨床ストレス応答学会大会
  • Place of Presentation: 東京農工大学（東京都小金井市）
  • Year and Date: 2015-11-07
  • Invited
• [Presentation] ノンコーディングRNAによる核内アーキテクチュア (2015)
  • Author(s): 廣瀬哲郎
  • Organizer: Aging, Bone and Cnacer Frontline Forum
  • Place of Presentation: 庭のホテル（東京都千代田区）
  • Year and Date: 2015-05-25
  • Invited
• [Presentation] RNA機能と神経変性 (2015)
  • Author(s): 廣瀬哲郎
  • Organizer: 日本神経学会北海道支部
  • Place of Presentation: 北海道大学（北海道・札幌市）
  • Year and Date: 2015-03-08
  • Invited
• [Presentation] Subnuclear architecture by long noncoding RNAs (2014)
  • Author(s): Tetsuro Hirose
  • Organizer: 理研セミナー
  • Place of Presentation: 理化学研究所横浜キャンパス（神奈川県・横浜市）
  • Year and Date: 2014-12-08
  • Invited
• [Presentation] Search for RNA-dependent subnuclear structures in mammalian cells (2014)
  • Author(s): Tetsuro Hirose
  • Organizer: Tokyo RNA Club
  • Place of Presentation: 東京大学（東京都・文京区）
  • Year and Date: 2014-11-28
  • Invited
• [Presentation] The possible common mechanism underlying assembly of the nuclear bodies on architectural long noncoding RNAs (2014)
  • Author(s): Tetsuro Hirose
  • Organizer: Japan-Australia joint meeting
  • Place of Presentation: シドニー（オーストラリア）
  • Year and Date: 2014-11-02 – 2014-11-05
  • Invited
• [Presentation] The common mechanism underlying assembly of the nuclear granules on the architectural noncoding RNAs (2014)
  • Author(s): Tetsuro Hirose
  • Organizer: EMBO-ESF conference
  • Place of Presentation: プルタスク（ポーランド）
  • Year and Date: 2014-10-14 – 2014-10-17
• [Presentation] ノンコーディングRNAネオタクソノミに向けた課題 (2014)
  • Author(s): 廣瀬哲郎
  • Organizer: RNAバイオインフォマティクス道場
  • Place of Presentation: 産業技術総合研究所北海道センター（北海道・札幌市）
  • Year and Date: 2014-08-26 – 2014-08-28
  • Invited
• [Presentation] PAR-CLIP法を用いた核内RNA顆粒の構造構築RNAの探索 (2014)
  • Author(s): 萬年 太郎、五島 直樹、油谷 浩幸、塩見 春彦、廣瀬 哲郎
  • Organizer: 日本RNA学会年会
  • Place of Presentation: ウインクあいち（愛知県・名古屋市）
  • Year and Date: 2014-07-23 – 2014-07-25
• [Presentation] 難溶性RNAの解析：NEAT1 ncRNAの細胞内再定量と新しいRNA群の探索 (2014)
  • Author(s): 中條 岳志、廣瀬 哲郎
  • Organizer: 日本RNA学会年会
  • Place of Presentation: ウインクあいち（愛知県・名古屋市）
  • Year and Date: 2014-07-23 – 2014-07-25
• [Presentation] クロマチン再構築複合体はncRNAに依存した複数の核内RNA構造体形成に必要である (2014)
  • Author(s): 川口 哲哉、谷川 明恵、長沼 孝雄、木村 宏、大川 恭行、Sylvie Souquere、Gerard Pierron、廣瀬 哲郎
  • Organizer: 日本RNA学会年会
  • Place of Presentation: ウインクあいち（愛知県・名古屋市）
  • Year and Date: 2014-07-23 – 2014-07-25
• [Presentation] Architectural noncoding RNAによる細胞内構造構築 の分子機構とその意義 (2014)
  • Author(s): 廣瀬哲郎
  • Organizer: 日本生化学会北海道支部会
  • Place of Presentation: 北海道大学（北海道・札幌市）
  • Year and Date: 2014-07-18
  • Invited
• [Presentation] Architectural noncoding RNAs controlling subnuclear organization and gene expression (2014)
  • Author(s): Tetsuro Hirose
  • Organizer: 第９回附置研究所ネットワーク国際シンポジウム
  • Place of Presentation: 大阪大学（大阪府・吹田市）
  • Year and Date: 2014-06-19 – 2014-06-21
• [Presentation] ノンコーディングRNAによる核内構造体形成機構 (2014)
  • Author(s): 廣瀬哲郎
  • Organizer: 日本細胞生物学会シンポジウム
  • Place of Presentation: 奈良県新公会堂（奈良県・奈良市）
  • Year and Date: 2014-06-10 – 2014-06-12
  • Invited
• [Book] 核内ボディの骨格として働く構造構築RNA(arcRNA) ノンコーディングRNA～RNA分子の全体像を俯瞰する～ (2016)
  • Author(s): 中條岳志、廣瀬哲郎
  • Total Pages: 372
  • Publisher: 化学同人
• [Book] Long Noncoding RNAs: Structures and Functions (2015)
  • Author(s): Mannen, T., Chujo, T., Hirose, T
  • Total Pages: 22
  • Publisher: Springer