| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2016: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
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| Outline of Final Research Achievements |
Accurate gene expression is the backbone of life phenomenon, and its breakdown and abnormality cause various diseases. In this research project, we aim to elucidate the degradation mechanism (NMPD) of gene products derived from abnormal mRNA, which is the earliest protein quality control mechanism. We analyzed short chain type proteolytic mechanism derived from abnormal mRNA with nonsense mutation, and obtained the following results. Sse1, an exchange factor for ADP/ATP of Hsp70, was required for promoting degradation of short chain type abnormal protein by Upf1. Mutant analysis of Sse1 revealed that interaction with Hsp70 is essential for NMPD. Moreover, it became clear that Sse1 does not promote the ubiquitination itself of short chain type abnormal protein itself. This study has led to an understanding of the molecular mechanism of NMPD, a degradation of truncated protein derived from abnormal mRNA with nonsense mutation.
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