| Project/Area Number |
26291004
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
阿部 義人 九州大学, 薬学研究科(研究院), 准教授 (60315091)
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| Co-Investigator(Renkei-kenkyūsha) |
KAWAKAMI Hironori 九州大学, 大学院薬学研究院, 助教 (50403952)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Keywords | 遺伝学 / ゲノム / 蛋白質 / 細菌 / 分子機械 / DnaA / 複製開始制御 / 高次複合体 / 遺伝子 / DNA複製 |
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| Outline of Final Research Achievements |
This study functionally and structurally analyzed, using various in vitro reconstitution assays, replication initiation complexes in Escherichia coli and complexes which regulate activity of DnaA protein, a major factor in replication initiation complexes. First, important mechanisms supporting complex formation of DnaA protein and DNA unwinding function in replication initiation complexes were revealed. Second, important mechanisms supporting complex formation of DnaA protein with chromosomal non-coding DNA elements, DARS and datA, as well as functional coupling of these complexes with the cell cycle were revealed.
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