| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Outline of Final Research Achievements |
Transcription-coupled nucleotide-excision repair (TC-NER) is one of the versatile DNA repair process that removes major UV-induced DNA lesions from actively transcribed genes. To investigate the initiation process of TC-NER reaction, we focused on a molecular mechanism that involves DNA-damage induced ubiquitination of RNA polymerase IIo (RNA pol IIo). The process is dependent on a recently identified TC-NER factor, UVSSA.
To determine the ubiquitination sites, we performed in vitro ubiquitination assay as well as SILAC-based High-Resolution Accurate Mass (HRAM) spectrometry. From the studies, we identified UVSSA-dependent ubiquitination sites required for the efficient TC-NER activity.
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