Molecular structures of the nuclear pore complex in Tetrahymena
| Project/Area Number |
26291007
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | National Institute of Information and Communications Technology |
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Principal Investigator |
Haraguchi Tokuko 国立研究開発法人情報通信研究機構, 未来ICT研究所・フロンティア創造総合研究室, 主任研究員 (20359079)
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| Research Collaborator |
OBUSE CHIKASHI 北海道大学, 先端生命科学研究科(研究院), 教授 (00273855)
IWAMOTO MASAAKI 国立研究開発法人情報通信研究機構, 未来ICT研究所・フロンティア創造総合研究室, 主任研究員 (80450683)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Keywords | 細胞・組織 / 生体分子 / 蛋白質 / 発生・分化 / 細胞構造 / 核構造 / 核膜孔複合体 / 核膜 / タンパク質 / 核機能 / 蛍光イメージング / 核分化 |
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| Outline of Final Research Achievements |
A ciliated Tetrahymena has two structurally and functionally distinct nuclei, a somatic macronucleus (MAC) and a germline micronucleus (MIC) that develop from the post-zygotic nuclei originated from MIC during the sexual process of conjugation. This study aims to understand molecular and structural difference between MAC and MIC nuclear pore complexes (NPCs), which is believed to be involved in the nuclear differentiation. By combining in silico analysis, mass spectrometry analysis for immuno-isolated proteins, and subcellular localization analysis of GFP fused proteins, we first identified all components of the NPCs in MAC and MIC and indicated structural difference between MAC and MIC NPCs. We next examined dynamic process of the nuclear differentiation using fluorescence live cell imaging and Live CLEM imaging, and found that assembly of the type-specific NPC is one of the most important determinants for the nuclear differentiation.
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Report
(4 results)
Research Products
(73 results)
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[Journal Article] Performance Evaluation of Leader-follower-based Mobile Molecular Communication Networks for Target Detection Applications.2016
Author(s)
Nakano T, Okaie Y, Kobayashi S, Koujin T, Chan C-H, Hsu Y-H, Okaie Y, Obuchi T, Hara T, Hiraoka Y, Haraguchi T
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Journal Title
IEEE Transactions on Communications
Volume: 65-2
Pages: 663-676
Related Report
Peer Reviewed
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[Journal Article] A genetically encoded probe for live-cell imaging of H4K20 monomethylation2016
Author(s)
Sato Y., Kujirai T., Arai R., Asakawa H., Ohtsuki C., Horikoshi N., Yamagata K., Ueda J., Nagase T., Haraguchi T., Hiraoka Y., Kimura A., Kurumizaka H., Kimura, H.
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Journal Title
J. Mol. Biol.
Volume: 428
Issue: 20
Pages: 3885-3902
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Inner nuclear membrane protein Lem2 augments heterochromatin formation in response to nutritional conditions.2016
Author(s)
Tange Y, Chikashige Y, Takahata S, Kawakami K, Higashi M, Mori C, Kojidani T, Hirano Y, Asakawa H, Murakami Y, Haraguchi T, Hiraoka Y.
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Journal Title
Genes to Cells
Volume: 21
Issue: 8
Pages: 812-832
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] BAF is a cytosolic DNA sensor that leads to exogenous DNA avoiding autophagy.2015
Author(s)
Kobayashi,S., Koujin, T., Kojidani, T., Osakada, H., Mori, C., Hiraoka, Y., Haraguchi, T.
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Journal Title
Proceedings of the National Academy of Sciences of the United States of America
Volume: 印刷中
Issue: 22
Pages: 7027-7032
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Deficiency of a lipid droplet protein, Perilipin 5, suppresses myocardial lipid accumulation, thereby preventing diabetes-induced heart malfunction2014
Author(s)
Kenta Kuramoto, Fumie Sakai, Nana Yoshinori, Tomoe Y. Nakamura, Shigeo Wakabayashi, Tomoko Kojidani, Tokuko Haraguchi, Fumiko Hirose,Takashi Osumi
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Journal Title
Molecular and Cellular Biology
Volume: 34(14)
Issue: 14
Pages: 2721-2731
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] 生細胞イメージング2015
Author(s)
原口徳子
Organizer
第25回細胞生物学ワークショップ
Place of Presentation
情報通信研究機構（兵庫県・神戸市）
Year and Date
2015-07-29
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