| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
|
|---|
| Outline of Final Research Achievements |
A ciliated Tetrahymena has two structurally and functionally distinct nuclei, a somatic macronucleus (MAC) and a germline micronucleus (MIC) that develop from the post-zygotic nuclei originated from MIC during the sexual process of conjugation. This study aims to understand molecular and structural difference between MAC and MIC nuclear pore complexes (NPCs), which is believed to be involved in the nuclear differentiation. By combining in silico analysis, mass spectrometry analysis for immuno-isolated proteins, and subcellular localization analysis of GFP fused proteins, we first identified all components of the NPCs in MAC and MIC and indicated structural difference between MAC and MIC NPCs. We next examined dynamic process of the nuclear differentiation using fluorescence live cell imaging and Live CLEM imaging, and found that assembly of the type-specific NPC is one of the most important determinants for the nuclear differentiation.
|
