Structural study of a huge respiratory supermolecule hemocyanin
| Project/Area Number |
26291008
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
Tanaka Yoshikazu 北海道大学, 先端生命科学研究科(研究院), 准教授 (20374225)
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| Co-Investigator(Kenkyū-buntansha) |
姚 閔 北海道大学, 先端生命科学研究科(研究院), 教授 (40311518)
加藤 公児 北海道大学, 先端生命科学研究科(研究院), 助教 (30452428)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Keywords | X線結晶構造解析 / ヘモシアニン / 巨大蛋白質会合体 / 酸素運搬 / 糖鎖 / タンパク質 / 構造解析 / 蛋白質 / X線結晶解析 / 軟体動物 / 巨大分子 |
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| Outline of Final Research Achievements |
In this study, we have determined the X-ray crystal structure of intact hemocyanin from Japanese flying squid at 3.0 angstrom resolution. The structure urevealed the architecture of hemocyanin composed of a cylindrical wall region and five collar regions, in which the dimers of functional units (FUs) hierarchically associated to form the entire decamer. Furthermore, the roles of carbohydrates in assembly, and conserved sulfur-containing residues in copper incorporation, were revealed.
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Report
(4 results)
Research Products
(41 results)
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[Journal Article] Biochemical and structural characterization of oxygen-sensitive 2-thiouridine synthesis catalyzed by the iron-sulfur protein TtuA2017
Author(s)
Minghao Chen, Shin-ichi Asai, Shun Narai, Shusuke Nambu, Naoki Omura, Yuriko Sakaguchi, Tsutomu Suzuki, Masao Ikeda-Saito, Kimitsuna Watanabe, Min Yao, Naoki Shigi and, Yoshikazu Tanaka
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 印刷中
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Crystal Structure of the 3.8-MDa Respiratory Supermolecule Hemocyanin at 3.0 Å Resolution2015
Author(s)
Zuoqi Gai, Asuka Matsuno, Koji Kato, Sanae Kato, Md Rafiqul Islam Khan, Takeshi Shimizu, Takeya Yoshioka, Yuki Kato, Hideki Kishimura, Gaku Kanno, Yoshikatsu Miyabe, Tohru Terada Yoshikazu Tanaka, and Min Yao
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Journal Title
Structure
Volume: 23
Issue: 12
Pages: 2204-2212
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] 巨大タンパク質会合体ヘモシアニンの多孔質性結晶を用いた生体分子の包摂2016
Author(s)
Asuka Matsuno, Ye Yuxin, Yuki Ohnishi, Akira Kitamura, Masataka Kinjo, Satoshi Abe, Takafumi Ueno, Yoshikazu Tanaka, Min Yao
Organizer
第54回日本生物物理学会年会
Place of Presentation
つくば国際会議場(茨城県，つくば市)
Year and Date
2016-11-25
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