Development of the micro-flow ultra-rapid pulse label NMR apparatus and elucidation of the prion self-replication process.
| Project/Area Number |
26291011
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Gifu University |
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Principal Investigator |
Kuwata Kazuo 岐阜大学, 大学院連合創薬医療情報研究科, 教授 (00170142)
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| Co-Investigator(Kenkyū-buntansha) |
鎌足 雄司 岐阜大学, 学内共同利用施設等, 助教 (70342772)
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| Co-Investigator(Renkei-kenkyūsha) |
FUKUOKA Mayuko 岐阜大学, 大学院連合創薬医療情報研究科, 特任助教 (60790988)
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| Research Collaborator |
HONDA Ryo
Ferdausi Ali
Kabir Aurangazeb
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
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| Keywords | NMR / 混合器 / パルスラベル / プリオン / 自己複製 / マイクロ流路 |
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| Outline of Final Research Achievements |
To perform the rapid mixing in the NMR magnet and to observe the folding or aggregation reaction of a protein in the atomic resolution, we devised an apparatus for the mixing of two solutions. Denatured protein solution goes through a first tubing and refolding buffer second tubing, and both tubing are connected to a carefully designed mixer in a NMR tube in the magnet. Waste solution is removed from the third tubing thereby enabling the observation in a continuous flow in the NMR tube. We reformed the apparatus used for in cell NMR using the glass material with homogeneous magnetic susceptibility with the aqueous solution. Thus we could remove the false peaks originated from the inhomogeneous magnetic susceptibility that was observed in the previous version of the apparatus. Also we found the ‘A-state’ which corresponds to the branching point between the cellular form and the aggregate, and that the cellular form itself inhibits the aggregate formation uncompetitively.
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Report
(4 results)
Research Products
(44 results)
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[Journal Article] Instability of C154Y variant of aldo-keto reductase 10.1038/s41598-017-00710-x1C32017
Author(s)
Satoshi Endo, Sayaka Takada, Ryo P. Honda, Kathrin Muller, Jochen H. Weishaupt, Peter M. Andersen, Albert C. Ludolph, Yuji O. Kamatari, Toshiyuki Matsunaga, Kazuo Kuwata, Ossama El-Kabbani, Akira Ikari
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Journal Title
Chemico-Biological Interactions
Volume: S0009-2797(16)
Pages: 194-202
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] A Novel Potent and Highly Specific Inhibitor against Influenza Viral N1-N9 Neuraminidases: Insight into Neuraminidase-inhibitor Interactions2016
Author(s)
Nongluk Sriwilaijaroen, Sadagopan Magesh, Akihiro Imamura, Hiromune Ando, Hideharu Ishida, Miho Sakai, Erika Ishitsubo, Takanori Hori, Setsuko Moriya, Takeshi Ishikawa, Kazuo Kuwata, Takato Odagiri, Masato Tashiro, Hiroaki Hiramatsu, Kenji Tsukamoto, Taeko Miyagi, Hiroaki Tokiwa, Makoto Kiso, and Yasuo Suzuki
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Journal Title
J.Med.Chem.
Volume: 59
Issue: 10
Pages: 4563-4577
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] 神経変性疾患と‘かたち’の制御2015
Author(s)
桑田一夫
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Journal Title
Clinical Neuroscience月刊 臨床神経科学 言語の起源と脳の進化 Vol.33 8 962-963
Volume: Vol.33 8
Pages: 962-963
Related Report
Peer Reviewed
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