In situ Protein NMR Structure Analysis
Project/Area Number |
26291013
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Structural biochemistry
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Research Institution | Yokohama National University (2016-2017) Osaka University (2014-2015) |
Principal Investigator |
Kojima Chojiro 横浜国立大学, 大学院工学研究院, 教授 (50333563)
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Co-Investigator(Kenkyū-buntansha) |
藤原 敏道 大阪大学, たんぱく質研究所, 教授 (20242381)
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Co-Investigator(Renkei-kenkyūsha) |
MATSUKI Yoh 大阪大学, 蛋白質研究所, 准教授 (70551498)
SUGIKI Toshihiko 大阪大学, 蛋白質研究所, 特任助教 (70635698)
FURUITA Kyoko 大阪大学, 蛋白質研究所, 特任助教 (30727665)
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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Keywords | NMR / 蛋白質 / 立体構造 / in situ |
Outline of Final Research Achievements |
For in situ protein NMR structure analysis, we have developed the following NMR techniques. Amino acid selective 13C-labeling and 13C-scrambling profile analysis of protein alpha and side-chain carbons in E. coli utilized for protein NMR. Protein 19F-labeling using transglutaminase for the NMR study of intermolecular interactions. Utilization of paramagnetic relaxation enhancements for high-resolution NMR structure determination of a soluble loop-rich protein with sparse NOE distance restraints.
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Report
(5 results)
Research Products
(36 results)
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[Journal Article] Publication of nuclear magnetic resonance experimental data with semantic web technology and the application thereof to biomedical research of proteins2016
Author(s)
Masashi Yokochi, Naohiro Kobayashi, Eldon L. Ulrich, Akira R. Kinjo, Takeshi Iwata, Yannis E. Ioannidis, Miron Livny, John L. Markley, Haruki Nakamura, Chojiro Kojima, and Toshimichi Fujiwara
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Journal Title
J. Biomed. Semant.
Volume: 7
Pages: 16-16
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] The Structure Determination of AgI-mediated Cytosine-Cytosine Base Pair within DNA Duplex in Solution with 1H/15N/109Ag NMR Spectroscopy2016
Author(s)
Takenori Dairaku, Kyoko Furuita, Hajime Sato, Jakub Sebera, Katsuyuki Nakashima, Jiro Kondo, Daichi Yamanaka, Yoshinori Kondo, Itaru Okamoto, Akira Ono, Vladimir Sychrovsky, Chojiro Kojima, and Yoshiyuki Tanaka
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Journal Title
Chemistry
Volume: 22
Issue: 37
Pages: 13028-13031
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Noncovalent forces tune the electron transfer complex between ferredoxin and sulfite reductase to optimize enzymatic activity2016
Author(s)
Ju Yaen Kim, Misaki Kinoshita, Satoshi Kume, Guy T. Hanke, Toshihiko Sugiki, John E. Ladbury, Chojiro Kojima, Takahisa Ikegami, Genji Kurisu, Yuji Goto, Toshiharu Hase, and Young-Ho Lee
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Journal Title
Biochem. J.
Volume: 473
Pages: 3837-3854
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Direct Detection of the Mercury-Nitrogen Bond in the Thymine-HgII-Thymine Base-pair with 199Hg NMR Spectroscopy2015
Author(s)
Takenori Dairaku, Kyoko Furuita, Hajime Sato, Jakub Sebera, Daichi Yamanaka, Hiroyuki Otaki, Shoko Kikkawa, Yoshinori Kondo, Ritsuko Katahira, F. Matthias Bickelhaupt, Celia Fonseca Guerra, Akira Ono, Vladimir Sychrovsky, Chojiro Kojima and Yoshiyuki Tanaka
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Journal Title
Chemical Communications
Volume: 51
Issue: 40
Pages: 8488-8491
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Physicochemical Nature of Interfaces Controlling Ferredoxin NADP+ Reductase Activity through Its Interprotein Interactions with Ferredoxin2015
Author(s)
Kinoshita Misaki, Kim Ju Yaen, Kume Satoshi, Sakakibara Yukiko, Sugiki Toshihiko, Kojima Chojiro, Kurisu Genji, Ikegami Takahisa, Hase Toshiharu, Kimata-Ariga Yoko, Lee Young-Ho
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Journal Title
Biochimica et Biophysica Acta-Bioenergetics
Volume: 1847
Issue: 10
Pages: 1200-1211
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] The crystal structure of the plant small GTPase OsRacl reveals its mode of binding to NADPH oxidase.2015
Author(s)
Kosami KI, Ohki I, Nagano M, Furuita F, Sugiki T, Kawano Y, Kawasaki T, Fujiwara T, Nakagawa A, Shimamoto K, Kojima C
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Journal Title
The Journal of Biological Chemistry
Volume: 289
Issue: 41
Pages: 28569-78
DOI
Description
(in press)(印刷中)
Related Report
Peer Reviewed
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[Journal Article] Bacterial effector modulation of host E3 ligase activity suppresses PAMP-triggered immunity in rice.2014
Author(s)
Ishikawa, K., Yamaguchi, K., Sakamoto, K., Yoshimura, S., Inoue, K., Tsuge, S., Kojima, C., Kawasaki, T.
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Journal Title
Nature Communications
Volume: 5
Issue: 1
Pages: 5430-5430
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] NMR tools developed for drug discovery2015
Author(s)
Chojiro Kojima
Organizer
International Symposium on Structure and Folding of Disease Related Proteins
Place of Presentation
Seoul National University (Seoul)
Year and Date
2015-12-04
Related Report
Int'l Joint Research / Invited
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