Elucidation of substrate recognition mechanism of I-CLiP in the trans- and juxta-membrane regions
| Project/Area Number |
26291016
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Yokohama City University |
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Principal Investigator |
NOGI Terukazu 横浜市立大学, 生命医科学研究科, 准教授 (40379102)
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| Co-Investigator(Kenkyū-buntansha) |
佐藤 毅 大阪大学, たんぱく質研究所, 講師 (90403013)
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| Co-Investigator(Renkei-kenkyūsha) |
AKIYAMA Yoshinori 京都大学ウイルス, 再生医科学研究所, 教授 (10192460)
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| Research Collaborator |
HIZUKURI Yohei
OI Rika
TAKANUKI Kazunori
TAMURA Risako
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2017: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
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| Keywords | タンパク質 / シグナル伝達 / 酵素 / 膜タンパク質 / 立体構造解析 / 蛋白質 / 膜蛋白質 |
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| Outline of Final Research Achievements |
In this project, we carried out structural and biochemical studies on intramembrane cleaving protease (I-CLiP) that catalyzes the regulated intramembrane proteolysis (RIP) so as to elucidate molecular mechanism by which I-CLiP recognizes its substrate membrane protein in the trans-membrane and juxta-membrane regions. Specifically, we studied on bacterial RsePs belonging to the site-2 protease family. It has been shown by our work that bacterial RsePs possess a characteristc intramembrane β-sheet implicated in the substrate binding. Our results contributed to a further understanding of conformational change of substrate membrane proteins within the transmembrane region.
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Report
(5 results)
Research Products
(23 results)
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[Journal Article] 3D structural analysis of protein O-mannosyl kinase, POMK, a causative gene product of dystroglycanopathy2017
Author(s)
M.Nagae, S.K.Mishra, M.Neyazaki, R.Oi, A.Ikeda, N.Matsugaki, S.Akashi, H.Manya, M.Mizuno, H.Yagi, K.Kato, T.Senda, T.Endo, T.Nogi, and Y.Yamaguchi
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Journal Title
Genes Cells
Volume: -
Issue: 4
Pages: 348-359
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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