Elucidation of substrate recognition mechanism of I-CLiP in the trans- and juxta-membrane regions
| Project/Area Number |
26291016
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Structural biochemistry
|
|---|
| Research Institution | Yokohama City University |
|---|
Principal Investigator |
NOGI Terukazu 横浜市立大学, 生命医科学研究科, 准教授 (40379102)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
佐藤 毅 大阪大学, たんぱく質研究所, 講師 (90403013)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
AKIYAMA Yoshinori 京都大学ウイルス, 再生医科学研究所, 教授 (10192460)
|
|---|
| Research Collaborator |
HIZUKURI Yohei
OI Rika
TAKANUKI Kazunori
TAMURA Risako
|
|---|
| Project Period (FY) |
2014-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2017: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
|
|---|
| Keywords | タンパク質 / シグナル伝達 / 酵素 / 膜タンパク質 / 立体構造解析 / 蛋白質 / 膜蛋白質 |
|---|
| Outline of Final Research Achievements |
In this project, we carried out structural and biochemical studies on intramembrane cleaving protease (I-CLiP) that catalyzes the regulated intramembrane proteolysis (RIP) so as to elucidate molecular mechanism by which I-CLiP recognizes its substrate membrane protein in the trans-membrane and juxta-membrane regions. Specifically, we studied on bacterial RsePs belonging to the site-2 protease family. It has been shown by our work that bacterial RsePs possess a characteristc intramembrane β-sheet implicated in the substrate binding. Our results contributed to a further understanding of conformational change of substrate membrane proteins within the transmembrane region.
|
Report
(5 results)
Research Products
(23 results)
-
-
-
[Journal Article] 3D structural analysis of proteinO-mannosyl kinase, POMK, a causative gene product of dystroglycanopathy2017
Author(s)
Nagae M, Mishra SK, Neyazaki M, Oi R, Ikeda A, Matsugaki N, Akashi S, Manya H, Mizuno M, Yagi H, Kato K, Senda T, Endo T, Nogi T, Yamaguchi Y
-
Journal Title
Genes Cells
Volume: 22
Pages: 348-359
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
