Function and dynamics of stress-induced lncRNA
| Project/Area Number |
26291018
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Ohta Kunihiro 東京大学, 大学院総合文化研究科, 教授 (90211789)
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| Co-Investigator(Kenkyū-buntansha) |
坪井 貴司 東京大学, 大学院総合文化研究科, 教授 (80415231)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Keywords | クロマチン / lncRNA / ヒストン修飾 / アンチセンスRNA / ノンコーディングRNA / 転写制御 / グルコース / 膵臓 / β細胞 / ストレス応答 / 発現制御 / ゲノム / 遺伝子発現 / マイクロアレイ |
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| Outline of Final Research Achievements |
We analyzed the role and dynamics of glucose-starved stress responsive long noncoding RNA (lncRNA) and antisense RNA (asRNA). We found the reciprocal expression control between lncRNA and asRNA synthesized in pairs and the histone H3K4 methylation-dependent control of asRNA expression. We observed transportation of asRNA to the cytoplasm and degradation of asRNA by nonsense-mediated degradation system. We also demonstrated that gene regulation is locally controlled by interaction between nascent lncRNA and Tup-type transcriptional repressor. In addition, we identified some lncRNAs associated with the downstream gene activation in mouse pancreatic β-cells.
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Report
(5 results)
Research Products
(98 results)
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[Journal Article] Replication stress induces accumulation of FANCD2 at central region of large fragile genes.2018
Author(s)
Okamoto Y, Iwasaki WM, Kugou K, Takahashi KK, Oda A, Sato K, Kobayashi W, Kawai H, Sakasai R, Takaori-Kondo A, Yamamoto T, Kanemaki MT, Taoka M, Isobe T, Kurumizaka H, Innan H, Ohta K, Ishiai M, Takata M.
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Journal Title
Nucleic Acids Res.
Volume: 46(6)
Issue: 6
Pages: 2932-2944
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Transcriptomes and Raman spectra are linked linearly through a shared low-dimensional subspace2017
Author(s)
Kobayashi-Kirschvink, K. J., Nakaoka, H., Oda, A., Kamei, K. F., Nosho, K., Fukushima, H., Kanesaki, Y., Yajima, S., Masaki, H., Ohta, K., Wakamoto, Y.
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Journal Title
bioRxiv
Volume: NA
Pages: 235580-235580
DOI
Related Report
Open Access
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[Journal Article] Shugoshin forms a specialized chromatin domain at subtelomeres that regulates transcription and replication timing.2016
Author(s)
Tashiro S, Handa T, Matsuda A, Ban T, Takigawa T, Miyasato K, Ishii K, Kugou K, Ohta K, Hiraoka Y, Masukata H, Kanoh J.
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Journal Title
Nature Commun.
Volume: 7
Issue: 1
Pages: 10393-10393
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Glycerophospholipid regulation of modality-specific sensory axon guidance in the spinal cord.2015
Author(s)
Guy AT, Nagatsuka Y, Ooashi N, Inoue M, Nakata A, Greimel P, Inoue A, Nabetani T, Murayama A, Ohta K, Ito Y, Aoki J, Hirabayashi Y, Kamiguchi H
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Journal Title
Science
Volume: 349
Issue: 6251
Pages: 974-977
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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