Study on the mitochondria-sarcoplasmic reticulum 3D Ca crosstalk in cardiomyocytes
Project/Area Number |
26291019
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Functional biochemistry
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Research Institution | University of Fukui |
Principal Investigator |
Takeuchi Ayako 福井大学, 学術研究院医学系部門, 特命助教 (00378704)
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Co-Investigator(Kenkyū-buntansha) |
天野 晃 立命館大学, 生命科学部, 教授 (60252491)
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Research Collaborator |
IINO Satoshi 福井大学, 学術研究院医学系部門, 教授
HORIGUCHI Kazuhide 福井大学, 学術研究院医学系部門, 准教授
FUKAZAWA Yugo 福井大学, 学術研究院医学系部門, 教授
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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Keywords | ミトコンドリア / 筋小胞体 / 心室筋細胞 / 心房筋細胞 |
Outline of Final Research Achievements |
The purpose of the study was to clarify physiological roles of mitochondria-sarcoplasmic reticulum 3D Ca crosstalk in cardiomyocytes. From combination study of experiments and mathematical simulations, we obtained following results. 1. Parameters explaining mitochondria-sarcoplasmic reticulum localization were obtained by analyzing electron microscopy data on mouse cardiomyocytes. 2. In vitro localization analyses were performed using HEK293 cells and mouse cardiomyocytes and it was suggested that mitochondrial Na/Ca exchanger NCLX and sarcoplasmic reticulum Ca pump SERCA were localized in close proximity to each other. 3. A mathematical model of a detailed mitochondrial oxidative phosphorylation was newly constructed. The model predicted the contribution of Ca-dependent regulations of mitochondrial energy metabolism with various compositions of energy substrates. In addition, the roles of mitochondria-sarcoplasmic reticulum crosstalk were also analyzed.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Patient-Specific Human Induced Pluripotent Stem Cell Model Assessed with Electrical Pacing Validates S107 as a Potential Therapeutic Agent for Catecholaminergic Polymorphic Ventricular Tachycardia2016
Author(s)
Sasaki K, Makiyama T, Yoshida Y, Wuriyanghai Y, Kamakura T, Nishiuchi S, Hayano M, Harita T, Yamamoto Y, Kohjitani H, Hirose S, Chen J, Kawamura M, Ohno S, Itoh H, Takeuchi A, Matsuoka S, Miura M, Sumitomo N, Horie M, Yamanaka S, Kimura T
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Journal Title
PLoS One
Volume: 11
Issue: 10
Pages: e0164795-e0164795
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Dysregulation of a potassium channel, THIK-1, targeted by caspase-8 accelerates cell shrinkage2016
Author(s)
Sakamaki K, Ishii TM, Sakata T, Takemoto K, Takagi C, Takeuchi A, Morishita R, Takahashi H, Nozawa A, Shinoda H, Chiba K, Sugimoto H, Saito A, Tamate S, Satou Y, Jung SK, Matsuoka S, Koyamada K, Sawasaki T, Nagai T, Ueno N
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Journal Title
Biochim Biophys Acta
Volume: 1863
Issue: 11
Pages: 2766-2783
DOI
Related Report
Peer Reviewed
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[Presentation] ミトコンドリアモデル2015
Author(s)
竹内綾子,松岡達
Organizer
e-Heartシンポジウム
Place of Presentation
立命館大学 びわこ・くさつキャンパス(南草津)
Year and Date
2015-02-28
Related Report
Invited
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