| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2017: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2016: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2015: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
|
|---|
| Outline of Final Research Achievements |
The CRL4-Cdt2 ubiquitin ligase plays an important role to prevent re-replication by targeting Cdt1 for degradation. In this project, we demonstrated that C-terminus of Cdt2 contributes to its association with PCNA upon initiation of S phase for rapid Cdt1 degradation. Purified proteins confirmed that the interaction is direct. On the other hand, Cdt2 is phosphorylated by cyclin-CDKs, which appeared to be important to inhibit its interaction with PCNA. Thus, CRL4-Cdt2 activity is regulated through the cell cycle. In addition, we showed that in response to UV damage, not only nucleotide repair but also mismatch repair participate in Cdt1 degradation for efficient repair of UV damages.
|
