| Project/Area Number |
26291027
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Kyoto University |
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Principal Investigator |
Imamura Hiromi 京都大学, 生命科学研究科, 准教授 (20422545)
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| Co-Investigator(Renkei-kenkyūsha) |
FUJIWARA Takahiro 京都大学, 物質-細胞統合システム拠点, 講師 (80423060)
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| Research Collaborator |
YOSHIDA Tomoki 京都大学, 大学院生命科学研究科, 研究員
IWAKIRI Ryuta 京都大学, 大学院生命科学研究科, 大学院生
NISHIYAMA Sui 京都大学, 大学院生命科学研究科, 大学院生
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Keywords | ATP / バイオセンサー / 蛍光タンパク質 / ブリン受容体 / インスリン / プリン受容体 / ライブイメージング / 生物発光 / BRET / ATPシグナル / イメージング |
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| Outline of Final Research Achievements |
The aim of this work was to establish methods to visualize extracellular ATP, in order to elucidate extracellular ATP signaling inside a live tissue, including a pancreatic islet.
First, we succeeded in imaging extracellular ATP by using a FRET-based biosensor. We also succeeded in developing two new ATP biosensors; single FP-based "QUEEN" and BRET-based "BTeam". Although we could use these new biosensors to monitor intracellular ATP, several problems remained to be solved before applying them to extracellular ATP imaging.
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