Spatio-temporal analysis of extracellular ATP signaling of pancreatic islets
| Project/Area Number |
26291027
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Kyoto University |
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Principal Investigator |
Imamura Hiromi 京都大学, 生命科学研究科, 准教授 (20422545)
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| Co-Investigator(Renkei-kenkyūsha) |
FUJIWARA Takahiro 京都大学, 物質-細胞統合システム拠点, 講師 (80423060)
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| Research Collaborator |
YOSHIDA Tomoki 京都大学, 大学院生命科学研究科, 研究員
IWAKIRI Ryuta 京都大学, 大学院生命科学研究科, 大学院生
NISHIYAMA Sui 京都大学, 大学院生命科学研究科, 大学院生
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Keywords | ATP / バイオセンサー / 蛍光タンパク質 / ブリン受容体 / インスリン / プリン受容体 / ライブイメージング / 生物発光 / BRET / ATPシグナル / イメージング |
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| Outline of Final Research Achievements |
The aim of this work was to establish methods to visualize extracellular ATP, in order to elucidate extracellular ATP signaling inside a live tissue, including a pancreatic islet.
First, we succeeded in imaging extracellular ATP by using a FRET-based biosensor. We also succeeded in developing two new ATP biosensors; single FP-based "QUEEN" and BRET-based "BTeam". Although we could use these new biosensors to monitor intracellular ATP, several problems remained to be solved before applying them to extracellular ATP imaging.
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Report
(4 results)
Research Products
(38 results)
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[Journal Article] The plasma membrane calcium pump in pancreatic cancer cells exhibiting the Warburg effect relies on glycolytic ATP2015
Author(s)
James AD, Patel W, Butt Z, Adiamah M, Dakhel R, Latif A, Uggenti C, Swanton E, Imamura H, Siriwardena AK, Bruce JI
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Journal Title
J. Bio. Chem.
Volume: 290
Issue: 41
Pages: 24760-24771
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Mitochondrial dysfunction in primary human fibroblasts triggers an adaptive cell survival program that requires AMPK-α2015
Author(s)
Distelmaier F, Valsecchi F, Liemburg-Apers DC, Lebiedzinska M, Rodenburg RJ, Heil S, Keijer J, Fransen J, Imamura H, Danhauser K, Seibt A, Viollet B, Gellerich FN, Smeitink JA, Wieckowski MR, Willems PH, Koopman WJ
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Journal Title
Biochim Biophys Acta
Volume: 1852
Issue: 3
Pages: 529-540
DOI
Related Report
Peer Reviewed
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[Journal Article] Toll-like receptor 9 protects non-immune cells from stress by modulating mitochondrial ATP synthesis through the inhibition of SERCA2.2014
Author(s)
Shintani Y, Drexler HC, Kioka H, Terracciano CM, Coppen SR, Imamura H, Akao M, Nakai J, Wheeler AP, Higo S, Nakayama H, Takashima S, Yashiro K, Suzuki K.
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Journal Title
EMBO Reports
Volume: 15
Issue: 4
Pages: 438-445
DOI
Related Report
Peer Reviewed / Open Access
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