Analysis of spaciotemporal regulation mechanisms for intracellular membrane remodeling in embryos
| Project/Area Number |
26291036
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Cell biology
|
|---|
| Research Institution | Gunma University |
|---|
Principal Investigator |
Sato Ken 群馬大学, 生体調節研究所, 教授 (30311343)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
原 太一 群馬大学, 生体調節研究所, 准教授 (00392374)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
|
|---|
| Keywords | 受精 / エンドサイトーシス / 分解 / オルガネラ形成・動態 / メンブレントラフィック / 線虫 / マウス / 細胞内分解 / 膜トラフィック |
|---|
| Outline of Final Research Achievements |
Fertilization triggers cell remodeling from each gamete to a totipotent zygote. In this study, we used the nematode C. elegans to understand the mechanisms underlying the cell remodeling during early development. We isolated several C. elegans mutants, which are defective in the cell remodeling during embryogenesis and found that one of these mutants has a mutation in the farnesyl pyrophosphate synthase gene. We also identified REI-1 as a novel regulator for a small GTPase Rab11 and showed that REI-1 functions as a guanine nucleotide exchange factor to recruit Rab11 to the Golgi apparatus for promoting the cytokinesis of embryos. In addition, we established a live-cell imaging system for mouse embryos and revealed that the cell remodeling such as the selective degradation of maternal membrane proteins also takes place in mammalian embryos.
|
Report
(4 results)
Research Products
(36 results)
-
-
-
-
-
-
[Journal Article] Guidelines for monitoring autophagy in Caenorhabditis elegans.2015
Author(s)
Zhang H, Chang JT, Guo B, Hansen M, Jia K, Kovács AL, Kumsta C, Lapierre LR, Legouis R, Lin L, Lu Q, Meléndez A, O'Rourke EJ, Sato K, Sato M, Wang X, Wu F.
-
Journal Title
Autophagy
Volume: 11
Pages: 9-27
Related Report
Peer Reviewed / Acknowledgement Compliant
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Book] WormBook2014
Author(s)
Sato K, Norris A, Sato M, Grant BD.
Publisher
The C. elegans Research Community
Related Report
-
-
