| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Outline of Final Research Achievements |
There are a variety of plasma membrane structures with various shapes. However, it is unclear how these structures are generated downstream of actin cytoskeleton. We found the BAR domain superfamily proteins that regulate the actin cytoskeleton through a variety of their protein surfaces that bind to the corresponding membrane-curvatures. CIP4 and GAS7 are unique F-BAR domain protein with their involvements in the protrusions, whereas the other F-BAR domains are involved in membrane invaginations such as clathrin-coated pits and caveolae. In this study, we found the membrane deformation of CIP4 for invagination is inhibited by the hetero-protein complex formation, thereby promoting the formation of membrane protrusions. In contrast, GAS7 F-BAR domain exhibited unique oligomeric assembly, forming the protein sheet for phagocytosis cup formation.
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