The role of RNA-binding protein for long-distance transportation of mRNA within embryonic neural progenitor cells

• Project/Area Number: 26291046
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Tohoku University
• Principal Investigator: OSUMI Noriko 東北大学, 医学(系)研究科(研究院), 教授 (00220343)
• Co-Investigator(Kenkyū-buntansha): 稲田 仁 東北大学, 医学(系)研究科(研究院), 講師 (60419893) ; 吉川 貴子 東北大学, 医学(系)研究科(研究院), 助教 (90727851)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000); Fiscal Year 2016: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2014: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: 神経発生 / RNA結合タンパク質 / FMRP / 放射状グリア / mRNA輸送 / 大脳皮質形成 / 放射状グリア細胞 / 大脳新皮質 / Pax6 / 大脳皮質 / Cyclin D2 / 哺乳類大脳皮質

Outline of Final Research Achievements

The mammalian cortex has expanded during evolution and its primordium consists with radial glial (RG) cells as neural progenitors. RG cells have long processes stretching between apical and basal surface of the cortical primordium. We would like to understand molecular mechanisms for long-distance transportation within the RG cell, and focused on an RNA-binding protein, i.e., fragile X mental retardation protein (FMRP) , coded by Fmr1 gene. We performed RIP-ChIP analyses and found candidate mRNAs as targets of FMRP, and examined expression profiles of the candidate genes. Immunohistochemical analyses showed decreased expression of some of the candidate genes at the protein level, suggesting a possibility that these transcripts can be transported by FMRP.

Research Products

• [Int'l Joint Research] British Columbia University(カナダ)
• [Int'l Joint Research] Harvard University(米国)
• [Journal Article] Electroporation in the Rodent Embryonic Brain Using Whole Embryo Culture System. (2017)
  • Author(s): Kikkawa T, Takahashi M, Osumi N
  • Journal Title: Curr Protoc Neurosci. Volume: 78 Issue: 1 Pages: 1-16
  • DOI: 10.1002/cpns.21
  • Peer Reviewed
• [Journal Article] Regional volume decreases in the brain of Pax6 heterozygous mutant rats: MRI deformation-based morphometry (2016)
  • Author(s): Hiraoka, K., Sumiyoshi, A., Nonaka, H., Kikkawa, T., Kawashima, R. and Osumi, N.
  • Journal Title: PLoS One Volume: 11 Issue: 6 Pages: e0158153-e0158153
  • DOI: 10.1371/journal.pone.0158153
  • Peer Reviewed / Open Access
• [Journal Article] Maternal dietary imbalance between omega-6 and omega-3 polyunsaturated fatty acids impairs neocortical development via epoxy metabolites. (2016)
  • Author(s): Sakayori N, Kikkawa T, Tokuda H, Kiryu E, Yoshizaki K, Kawashima H, Yamada T, Arai H, Kang JX, Katagiri H, Shibata H, Innis SM, Arita M, Osumi N.
  • Journal Title: Stem Cells. Volume: 34(2) Issue: 2 Pages: 470-482
  • DOI: 10.1002/stem.2246
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Horizontal basal cell-specific deletion of Pax6 impedes recovery of the olfactory neuroepithelium following severe injury. (2015)
  • Author(s): Suzuki J, Sakurai K, Yamazaki M, Abe M, Inada H, Sakimura K, Katori Y, Osumi N.
  • Journal Title: Stem Cells Dev. Volume: in press
  • Peer Reviewed / Open Access
• [Journal Article] Neurogenesis and sensorimotor gating: bridging a microphenotype and an endophenotype. (2015)
  • Author(s): Osumi N, Guo N, Matsumata M, Yoshizaki K.
  • Journal Title: Curr Mol Med. Volume: in press
  • Peer Reviewed
• [Journal Article] Preparation of rat serum suitable for mammalian whole embryo culture. (2014)
  • Author(s): Takahashi M, Makino S, Kikkawa T, Osumi N.
  • Journal Title: J Vis Exp. Volume: 90 Issue: 90
  • DOI: 10.3791/51969
  • Peer Reviewed
• [Journal Article] Asymmetric inheritance of Cyclin D2 maintains proliferative neural stem/progenitor cells: a critical event in brain development and evolution. (2014)
  • Author(s): Tsunekawa Y, Kikkawa T, Osumi N.
  • Journal Title: Dev Growth Differ. Volume: 522 Issue: 15 Pages: 3520-3528
  • DOI: 10.1002/cne.23621
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Dmrt genes regulate Cajal-Retzius cell development of the cerebral cortex. (2016)
  • Author(s): Kikkawa, T., Sakayori, N., Yuuki, H., Osumi, N.
  • Organizer: 第9回 神経発生討論会
  • Place of Presentation: 東京、東京医科歯科大学
  • Year and Date: 2016-03-19
• [Presentation] Pax6 is involved in the development of cortical Sp8 positive inhibitory cells (2016)
  • Author(s): Nakamura, R., Kikkawa, T., Sato, T., Osumi, N.
  • Organizer: 第9回 神経発生討論会
  • Place of Presentation: 東京、東京医科歯科大学
  • Year and Date: 2016-03-19
• [Presentation] Dmrt genes differentially participate in Cajal-Retzius cell development of the cerebral cortex. (2016)
  • Author(s): Kikkawa, T., Sakayori, N., Yuuki, H., Katsuyama, Y., Osumi, N.
  • Organizer: Neocortical Organization III
  • Place of Presentation: 東京、東京大学
  • Year and Date: 2016-02-11
  • Int'l Joint Research
• [Presentation] Pax6 is involved in inhibitory neuron production in the murine cerebral cortex (2015)
  • Author(s): Nakamura, R., Kikkawa, T., Sato, T., Osumi, N.
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸、神戸ポートアイランド
  • Year and Date: 2015-12-02
• [Presentation] Dmrt genes differentially participate in Cajal-Retzius cell development of the cerebral cortex. (2015)
  • Author(s): Kikkawa, T., Sakayori, N., Yuuki, H., Katsuyama, Y., Osumi, N.
  • Organizer: Society for Neuroscience 45th Annual Meeting
  • Place of Presentation: アメリカ・シカゴ
  • Year and Date: 2015-10-18
  • Int'l Joint Research
• [Presentation] Dmrt genes differentially participate in Cajal-Retzius cell development of the cerebral cortex. (2015)
  • Author(s): Kikkawa, T., Sakayori, N., Yuuki, H., Kastuyama, Y., Osumi, N.
  • Organizer: Tohoku Forum for Creativity, Development and Disease
  • Place of Presentation: 仙台、東北大学
  • Year and Date: 2015-08-25
  • Int'l Joint Research
• [Presentation] Molecular and cellular mechanisms of cortical development: Its significance in brain evolution and psychiatric diseases. (2014)
  • Author(s): Osumi N
  • Organizer: The 12th Meeting of the Asian-Pacific Society for Neurochemistry
  • Place of Presentation: 台湾
  • Year and Date: 2014-08-24
  • Invited
• [Book] 脳からみた自閉症 「障害」と「個性」のあいだ (2016)
  • Author(s): 大隅 典子
  • Total Pages: 256
  • Publisher: 講談社
• [Book] Electroporation Methods in Neuroscience (2015)
  • Author(s): Takahashi M, Kikkawa T, Osumi N
  • Total Pages: 199
  • Publisher: Humana Press
• [Remarks] 妊娠中の脂質摂取が仔マウスの脳形成と情動に与える影響
  • URL: http://www.tohoku.ac.jp/japanese/2015/11/press20151113-02.html
• [Remarks] 妊娠中の脂質摂取が仔マウスの脳形成と情動に与える影響（英語版）
  • URL: http://www.tohoku.ac.jp/en/press/fish_intake_by_pregnant_women.html
• [Remarks] 胎生期脳の幹細胞から神経細胞が生まれる仕組みの解明
  • URL: http://www.tohoku.ac.jp/japanese/2012/03/press20120302-05.html
• [Remarks] 東北大学脳科学センター市民講座「脳・神経の病気の解明はどこまで進んでいるか」（9/21開催）
  • URL: http://www.tohoku.ac.jp/japanese/2014/09/event20140829-02.html